Polymorphism of HLA‐A, ‐B, ‐DRB1, ‐DQA1 and ‐DQB1 haplotypes in a Croatian population

Abstract

We describe for the first time extended haplotypes in a Croatian population. The present study gives the HLA-A, -B, -DRB1, -DQA1 and -DQB1 allele and haplotype frequencies in 105 families with at least two offspring. All individuals were studied by conventional serology for HLA class I antigens (A and B), while class II alleles (DRB1, DQA1, DQB1) were typed using the PCR-SSOP method. HLA genotyping was performed by segregation in all 105 families. For extended haplotype analysis, 420 independent parental haplotypes were included. Fourteen HLA-A, 18 HLA-B, 28 DRB1, 9 DQA1 and 11 DQB1 alleles were found in the studied population. Most of the DRB1 alleles in our population had an exclusive association with one specific DQA1-DQB1 combination. This strong linkage disequilibrium within the HLA class II region is often extended to the HLA-B locus. A total of 10 HLA-A, -B, -DRB1, -DQA1, -DQB1 haplotypes were observed with a frequency </= 1.0%. The three most frequent haplotypes were HLA-A1, B8, DRB1*0301, DQA1*0501, DQB1*0201; HLA-A3, B7, DRB1*1501, DQA1*0102, DQB1*0602 and HLA-A24, B44, DRB1*0701, DQA1*0201, DQB1*02. These results should provide a useful reference for further anthropological studies, transplantation studies, and studies of associations between HLA and diseases.

Association Between Human Leukocyte Antigen ( HLA ) With Chronic Renal Disease

“…[19] Also, DRB11 rate was found 29% in Macedonians [20] and 15% in Croatians. [21] Temiz et al [14] reported that HLA Cw04 is the most and HLA cw 07 IS THE SECOND FREQUENT AL-LELE IN their population but we found that Cw07 is the most (28.7%) and Cw 04 is the second one (21.8%). Also, DQ allele results showed some dşffereneces with pur study.…”

Precise Identification of a Human Immunodeficiency Virus Type 1 Antigen Processing Mutant

“…Alleles HLA-A*03 and HLA-B*0702 are in strong linkage disequilibrium (8,17). The original study by Moore et al (31) did not detect an association between HLA-A*0301 and E169D independent of HLA-B*0702.…”

“…Position 169 also maps within an overlapping HLA-A*0301-restricted epitope, MR9 (aa 164 to 172). Despite the strong linkage disequilibrium between HLA-A*0301 and B*0702 and the high frequency of HLA A*03 in the population (8,17), statistical association between E169D and HLA-A*0301, independent of HLA-B*0702, has not been found.…”

Enumeration of Haemagglutinin‐specific CD8+ T Cells after Influenza Vaccination Using MHC Class I Peptide Tetramers

“…In total, 94 individuals (average age 32, range 22-59) were screened to obtain HLA-A*0201 + group of adult healthy volunteers. HLA-A*02 + status was tested using the microlymphocytotoxicity (MLCT) serological technique [13] and ⁄ or fluorescein isothiocyanate anti-HLA-A*02 antibody (Proimmune, Oxford, UK). HLA-A*0201 typing was performed using polymerase chain reaction with sequence-specific primers (PCR-SSP) as described previously [14].…”