Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat

Polley, Shamik; De, Sachinandan; Batabyal, Subhasis; Kaushik, Ravinder; Yadav, Paras; Arora, Jaspreet Singh; Chattopadhyay, S.; Pan, Subhransu; Brahma, Biswajit; Datta, Tirtha Kumar; Goswami, S. L.

doi:10.1016/j.smallrumres.2009.08.004

Cited by 55 publications

(65 citation statements)

References 28 publications

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“…The annealing sites of primer and restriction sites are presented in Figure 3. This result is in line with some researches, those were Boer goat, Boer Huanghuai, Haimen, Huanghai, Nubi dan Matou (Hua et al, 2008); Black Bengal goat (Polley et al, 2009); Iran local goat (Tajangookeh et al, 2009); Marwari goat (Godara et al, 2012); Indian local goat (Ahlawat et al, 2013); Markhoz goat (Shokrollahi, 2015). In contrast, the polymorphisms were found in Batang goat (Hidayat et al, 2015), White goat (Ran et al, 2009), White Funiu and Black Taihang goat (Wang et al, 2011) and AngloNubian goat (Rahman et al, 2013).…”

Section: Resultssupporting

confidence: 87%

Identification of BMP15 Exon 2 for fecundity traits by PCR-RFLP and nucleotide sequencies in Kejobong goat

Febriana

Sutopo

Kurnianto

2017

J. Indonesian Trop. Anim. Agric.

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ABSTRAKKambing Kejobong dikenal sebagai bangsa kambing yang mempunyai sifat prolifik dan produktivitas yang tinggi di Indonesia. PCR-RFLP dan metode sekuensing digunakan pada penelitian ini untuk mengetahui keragaman gen BMP15 ekson 2 pada kambing Kejobong. Sampel darah diperoleh dari 48 induk kambing Kejobong yang diseleksi berdasarkan litter sizenya. Ukuran fragmen hasil amplifikasi PCR gen BMP15 ekson 2 adalah 837 pb. Amplikon PCR-RFLP direstriksi menggunakan enzim HinfI menghasilkan sampel yang monomorfik. Verifikasi hasil menggunakan metode sekuensing menghasilkan 4 substitusi (A391G, C464G, T828C and C830G), 1 alignment gap (situs ke-817) and 1 insersi nukelotida (situs ke-822). Mutasi ini menyebabkan terbentuknya 6 haplotipe. Mutasi gen BMP15 ekson 2 pada kambing Kejobong mengindikasikan bahwa bangsa ini mempunyai pola mutasi berbeda yang mengontrol sifat prolifik. Pohon filogeni yang disusun berdasarkan sekuen gen BMP15 ekson 2 pada kambing Kejobong membentuk 3 klaster. Alignment gap memberikan penciri khusus bagi induk kambing Kejobong yang memiliki litter size 2 (duplet). Insersi diduga menjadi situs pengenalan kambing Kejobong berdasarkan gen BMP15 ekson 2.Kata Kunci: sifat fekunditas, polimorfisme, gen BMP15, prolifik, kambing Kejobong ABSTRACTKejobong goat is known as prolific and high productivity goat breed in Indonesia. PCR-RFLP and sequencing technique was established in the present study to accomplish the polymorphisms of Bone Morphogenetic Protein 15 (BMP15) gene exon 2 on Kejobong goat does. The blood samples was collected from 48 Kejobong does which were selected based on their litter size. The size of PCR amplification of BMP15 gene exon 2 was 837 bp. The product of PCR-RFLP technique digested by HinfI enzyme showed that the samples were monomorphic. Authentication result using nucleotide sequencing found 4 substitution (A391G, C464G, T828C and C830G), 1 alignment gap (site 817) and 1 insertion nucleotide (site 822). This mutations caused 6 haplotypes formatted. The mutants of BMP15 exon 2 on Kejobong goats indicated that this breed had their own mutation controling the prolific trait. The phylogenetic tree build on the sequences of BMP15 gene exon 2 of Kejobong goats was grouped into 3 clusters. The alignment gap indicated to be the specific marker for the prolific trait (duplet) in Kejobong goat. The particular insertion site could be the recognition site of Kejobong goat based on BMP15 exon 2.

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Section: Resultssupporting

confidence: 87%

Identification of BMP15 Exon 2 for fecundity traits by PCR-RFLP and nucleotide sequencies in Kejobong goat

Febriana

Sutopo

Kurnianto

2017

J. Indonesian Trop. Anim. Agric.

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“…In the Black Bengal goat, only wild type alleles were identified as having G, A, G, G and C nucleotides at the G1, G4, G6, G7 and G8 locations, which correspond to the Arginine, Glutamic acid, Valine and Serine amino acids, respectively. All animals in this study were wildtype homozygous for GDF9 (Polley et al, 2009). Results from the GDF9 analysis in the Blackbelly breed are congruent with the results of mutation analysis in G5, G6, G7 and G8 from different North African sheep breeds (Barbarine, Queue Fine de L'Ouest, Noire de Thibar, Sicilo-Sarde and D'man).…”

supporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Analysis of the 3´ End Regions of the Gdf9 and Bmpr1b Genes in Blackbelly Sheep From Yucatán, Mexico

López-Ramírez¹,

Magaña-Sevilla²,

Zamora-Bustillos³

et al. 2014

Cienc. Inv. Agr.

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The FecB mutation in the BMPR1B gene and the G6, G7 and G8 mutations in the GDF9 gene have been documented in Booroola Merino sheep breeds. These mutations have all been identified in several ovine breeds, such as Belclare, Cambridge. Blackbelly hairy sheep breed, which is well adapted to tropical climates due to its African origin, have 2 to 3 lambs per litter, corresponding to a high prolificacy for a tropical breed. The objective of this investigation was to identify mutations in the cDNA of the BMPR1B and GDF9 genes of 20 Blackbelly ewes selected for their prolificacy (two or more lambs by birth). The results of this study indicate the absence of mutations in these genes from the Blackbelly breed. However, it is important to analyze the 3' end region of the cDNA of these genes to rule out the presence or absence of other mutations related to prolificacy. (Mulsant et al., 2001). The FecB mutation in BM-PR1B consists of an A→G transition at nucleotide 746 in the open reading frame and is associated with the hyper-prolific phenotype of the Booroola sheep (Wilson et al., 2001;Souza et al., 2001). This mutation was subsequently reported in other sheep breeds around the world, including the Garole sheep (Polley et al., 2010), Japanese sheep (Davis et al., 2002), the Small Tailed Han sheep (Chu et al., 2007), Hu sheep (Wang et al., 2003) and others (Davis et al., 2006;Hua and Yang 2009). The GDF9 (Growth Differentiation Factor 9) gene maps to chromosome 5 and contains 2 exons (Bodensteiner et al., 1999). The gene extends through a genomic region of approximately 2.5 kb and contains two exons coding for one pro-peptide of 453 amino acids separated by a single intron of 1126 base pairs. In 2004, eight single base mutations (SNP) in the GDF9 gene (G1 to G8) were reported in Cambridge and Belclare ewes (Hanrahan et al., 2004). One mutation is located in exon 1, one is located in an intron and six mutations are in exon 2. Three SNPs do not alter the amino acid sequence; these SNPs are located at position 471 (G2), position 477 (G3) and position 978 (G5). Five of these SNPs result in a change of amino acid sequences (G1, G4, G6, G7 and G8).The objective of this investigation was to identify mutations in the BMPR1B and GDF9 cDNA sequences obtained from 20 Blackbelly ewes selected for their prolificacy. Materials and methods Isolation of total RNAFull blood Blackbelly ewes were selected from the breeding nucleus of Tecnológico de Conkal based on the number of lambs per birth. Twenty of those with a record of two or more lambs in each farrowing were used. Estrus was induced in these ewes over a period of 12 days by applying vaginal sponges impregnated with Chronogest® CR 7 (Flugestone acetate-acetosi-α-fluoro-11-β-hidroxi-pregn-4-ene-3, 20 diona). Two days later, these females were sedated with Ketamin (1.1 mL 50 kg -1 ) for quirurgical intervention, and ovarian tissue was biopsied. These biopsies were preserved in RNAlater and stored at -20 °C before being placed in 0.5 mL of TRIzol reagent (Invitrogen, Carlsbad CA) to be...

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“…Genetic information, particularly for those loci that affect performance traits, is an important tool in breeding programs. Extensive research has been carried out on different prolific goat breeds to identify the genes involved in the control of litter size (Polley et al, 2009). Several candidate genes and their relationship with litter size in goats have been studied, such as the G protein-coupled receptor 54 (GPR54) gene (Cao et al, 2011), cocaine-amphetamine-regulated transcript (CART) gene , kit ligand (KITLG) gene , bone morphogenetic protein receptor IB (BMPR-IB) gene and so on.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of KISS1 promoter and effect of KISS1 gene mutations on litter size in the goat

Han²,

Hou³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Kisspeptins, the product of the KISS1 gene, play an essential role in the regulation of reproductive functions, acting primarily at the hypothalamic level of the gonadotropic axis. We detected polymorphisms of the goat KISS1 gene in 723 individuals from three goat breeds (Xinong Saanen, Guanzhong, and Boer) by DNA pooling, PCR-RFLP, and DNA sequencing methods. We cloned the promoter sequence of this gene and found it to share high similarity with that of the bovine KISS1 promoter. Six TATA boxes were found in the goat KISS1 promoter region. Two novel SNPs (g.2124T>A and g.2270C>T) were identified in the intron 1 of the KISS1 gene of all three goat breeds. The three goat breeds were in Hardy-Weinberg disequilibrium at g.2124T>A and g.2270C>T loci. The g.2124T>A and g.2270C>T loci were closely linked in the three goat breeds (r 2 > 0.33). The g.2124T>A and g.2270C>T SNPs were significantly associated with litter size, and the C1 female goats had a larger litter size than did those with the other genotypes. These results extend the spectrum ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 4308-4316 (2013) Effect of KISS1 gene mutations on goat litter size 4309 of genetic variation of the goat KISS1 gene, which contributes to our knowledge of goat genetic resources for breeding programs.

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Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat

Cited by 55 publications

References 28 publications

Identification of BMP15 Exon 2 for fecundity traits by PCR-RFLP and nucleotide sequencies in Kejobong goat

Identification of BMP15 Exon 2 for fecundity traits by PCR-RFLP and nucleotide sequencies in Kejobong goat

Analysis of the 3´ End Regions of the Gdf9 and Bmpr1b Genes in Blackbelly Sheep From Yucatán, Mexico

Molecular cloning and characterization of KISS1 promoter and effect of KISS1 gene mutations on litter size in the goat

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