Polymorphism of FecB Gene in Nine Sheep Breeds or Strains and Its Effects on Litter Size, Lamb Growth and Development

Gao, Feng; Shou-ren, Liu; Guo-qing, Shi; Ai, Juntao; Mao, Dagan; Yang, Liguo

doi:10.1016/s0379-4172(06)60030-9

Cited by 38 publications

(34 citation statements)

References 14 publications

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“…The FecX G mutation was found to be absent in Hu sheep, although FecX G mutation (C718T) has been reported in Small-tailed Han, Belclare and Cambridge ewes (Chu et al 2005b). As the most famous high prolific sheep in China, Hu sheep has BB genotype in FecB, and no BMP15 mutation has been reported so far (Wang et al 2003;Davis et al 2006;Guan et al 2007). FecX I mutation was also not found in Chinese Merino (54), Xinjiang Fine Wool (35) and Romolly Hills (40; Dai 2004 Although there are many reports on mutations in a single gene of fecundity gene family, mutations at the multiple loci of fecundity genes have been reported in three sheep breeds only.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep

Polley

Brahma

et al. 2009

Trop Anim Health Prod

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Mutation studies in different prolific sheep breeds have shown that the transforming growth factor beta super family ligands viz. the growth differentiation factor 9 (GDF9/FecG), bone morphogenetic protein 15 (BMP15/FecX) and associated type I receptors, bone morphogenetic protein receptor (BMPR1B/FecB), are major determinant of ovulation rate and consequent increase in litter size. The Garole sheep is a highly prolific sheep breed of India. Characterization of fecundity genes in these animals could substantially improvise the breeding programme in these animals as well as other sheep breeds of the region. The present study was therefore designed with the objective of polymorphism study of fecundity genes in these prolific microsheep. A total of 11 point mutations were detected by polymerase chain reaction (PCR)-based method. A competitive technique called tetra-primer amplification refractory mutation system-PCR was adapted to type a total of ten points of two ovine fecundity genes (GDF9 and BMP15). The FecB locus of the BMPR1B gene and G1 locus of GDF9 gene were found to be polymorphic. In FecB locus, two genotypes, wild type (FecB(+)) and mutant (FecBB), were detected with allele frequencies of 0.39 and 0.61, respectively. At G1 locus, two genotypes, mutant (A) and wild types (G) were detected with allele frequencies of 0.18 and 0.82, respectively. This study reports Garole sheep as the fourth sheep breed after Belclare/Cambridge, Lacaune and Small-tailed Han sheep, where coexisting polymorphism has been found in two different fecundity genes (BMPRIB and GDF9 genes).

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Section: Discussionmentioning

confidence: 99%

Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep

Polley

Brahma

et al. 2009

Trop Anim Health Prod

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“…In some reports, the FecB gene has been implicated with lower birth weight and slower postweaning growth rate in lambs as well as a lighter mature body weight in ewes (Gootwine et al 2006). Contrary to this, FecB gene had been shown to have a positive effect on early postnatal body growth in Chinese Merino prolific meat strain (Guan et al 2007).…”

Section: Introductionmentioning

confidence: 72%

Polymorphism analysis of BMPR1B gene by forced RFLP and PCR-SSCP techniques and expression of the mutation in introgressed sheep

Ganai

Misra

Shabir

2011

Trop Anim Health Prod

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The present study was conducted to screen Kashmir valley sheep with history of prolificacy for the presence of FecB mutation. Forced polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single strand conformation polymorphism (SSCP) techniques were employed to detect any polymorphism present in bone morphogenetic protein receptor type 1B (BMPR1B) gene. Further, it was aimed at introgressing the FecB mutation into nonprolific noncarrier sheep. A 140-bp fragment of BMPR1B gene was amplified from isolated genomic DNA and subjected to forced RFLP with restriction enzyme AvaII. Three different RFLP patterns were identified. SSCP analysis showed one-to-one correspondence with RFLP patterns. Sequencing of the samples showing different patterns revealed that the wild (+) and mutant (B) alleles were different by a single nucleotide substitution in the form of A109G from wild to mutant allele. It led to change in amino acid from Glutamine (Q) to Arginine (R) from wild to mutant allele. The mutation was only detected in NARI-Suwarna and their crosses; all Kashmir valley sheep with prolific history lacked it. The + allele was abundant in the studied population. The FecB mutation was introgressed in nonprolific noncarrier sheep by crossing ewes with NARI-Suwarna rams possessing the mutation. First generation crossing produced heterozygous (B+) progeny. Some of the F(1) heterozygous ewes gave birth to twins when mated to unrelated NARI-Suwarna rams. It showed that FecB mutation was successfully expressing in those crosses.

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“…Once a forced restriction site is introduced into one of a pair of primers, the PCR product will contain a certain restriction enzyme site. The F-RFLP has become a rapid, simple, and definitive technique for SNP detection, and was used previously to ge-62 R-L. Han et al notype prolific sheep (Davis et al 2002;Guan et al 2006;Hua et al 2008) and swine (Kim et al 2003). The results showed that F-RFLP is a robust and accurate method for genotyping SNPs.…”

Section: Discussionmentioning

confidence: 99%

A novel single-nucleotide polymorphism of thevisfatin gene and its associations with performance traits in the chicken

et al. 2010

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Visfatin is a peptide that is predominantly expressed in visceral adipose tissue and is hypothesized to be related to obesity and insulin resistance. In this study, a novel silent single-nucleotide polymorphism (SNP) was found in exon 7 of the chicken visfatin gene (also known as PBEF1) by single-stranded conformation polymorphism (SSCP) and DNA sequencing. In total, 836 chickens forming an F2 resource population of Gushi chicken crossed with Anka broiler were genotyped by XbaI forced RFLP, and the associations of this polymorphism with chicken growth, carcass characteristics, and meat quality were analyzed. Significant associations were found between the polymorphism and 4-week body weight (BW4), 6-week body weight (BW6), 4-week body slanting length (BSL4), fat bandwidth (FBW), breast muscle water loss rate (BWLR) and breast muscle fiber density (BFD) (P < 0.05), as well as 4-week breastbone length (BBL4) (P < 0.01). These observations suggested that the polymorphism in exon7 of the visfatin gene had significant effects on the early growth traits of chicken.

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Polymorphism of FecB Gene in Nine Sheep Breeds or Strains and Its Effects on Litter Size, Lamb Growth and Development

Cited by 38 publications

References 14 publications

Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep

Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep

Polymorphism analysis of BMPR1B gene by forced RFLP and PCR-SSCP techniques and expression of the mutation in introgressed sheep

A novel single-nucleotide polymorphism of thevisfatin gene and its associations with performance traits in the chicken

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