Polymorphism of DNA double helices

Leslie, Andrew G. W.; Arnott, Struther; Chandrasekaran, R.; Ratliff, Robert L.

doi:10.1016/0022-2836(80)90124-2

Cited by 418 publications

(219 citation statements)

References 26 publications

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“…However, the A form was trapped in fibers of the Na+ salt up to 92% relative humidity [2,3], and it was monitored by CD spectroscopy if trifluoroethanol was added to an aqueous solution of poly d(G-C) -poly d(G-C) [7]. Under these latter conditions, slow transitions A-Z and B-Z were observed and a fast B-A transition which occurred in about 10 s. An A&Z transition has been described in the ribo series for poly r(G-C) -poly r(G-C)…”

Section: The Unique Cpg Step In A-dnamentioning

confidence: 99%

“…poly d(G-C). The A form was trapped in the fiber state [2,3] where it is probably stabilized by crystal packing forces. X-ray fiber diffraction clearly indicates that poly d(G-C) .…”

Section: Pimentioning

confidence: 99%

“…The Z-DNA form is also found in fibers under high-salt conditions [2,3]. NMR data suggest that the B-Z transition does not involve opening or breaking of the G-C Watson-Crick base pairs [6].…”

Section: Introduction Poly D(g-c) -Poly D(g-c) Adopts the Canonical Rmentioning

confidence: 98%

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Raison d'être and structural model for the B‐Z transition of poly d(G‐C)^∗poly d(G‐C)

Heinemann

1989

FEBS Letters

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In DNA oligonucleotides crystallized in the A form, the nucleotides adopt standard conformation except for steps 5'‐CpG‐3' where reduced base‐pair twist and a sliding motion of the base pairs along their long axes causes pronounced interstrand guanine‐guanine overlap. As a consequence, torsion angles α,β and γ are consistently trans, trans, trans instead of the common ‐gauche, trans, + gauche. This conformation significantly increases the intraresidue distance between the guanine base and the 5'‐phosphate group. A molecular model of poly d(G‐C)·poly d(G‐C) built with these structural characteristics in the A form, which we call A2‐DNA, shows that rotation of the guanosine sugar into the syn orientation is easily achieved and pushes the base pair across the helix axis. If successive guanosines are changed this way, a smooth transformation occurs to the left‐handed Z‐DNA. We suggest that A‐ and A2‐DNA forms of poly d(G‐C)·poly d(G‐C) are metastable and that the actual transition is B ⇌ (A ⇌ A2) ⇌ Z‐DNA.

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Section: The Unique Cpg Step In A-dnamentioning

confidence: 99%

“…poly d(G-C). The A form was trapped in the fiber state [2,3] where it is probably stabilized by crystal packing forces. X-ray fiber diffraction clearly indicates that poly d(G-C) .…”

Section: Pimentioning

confidence: 99%

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Raison d'être and structural model for the B‐Z transition of poly d(G‐C)^∗poly d(G‐C)

Heinemann

1989

FEBS Letters

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“…DNA is most commonly found in the B form under normal cellular conditions (1). The presence of DNA sequences that adopt conformation (s) other than the B form might enhance/suppress transcription when they are located near/in regulatory regions.…”

mentioning

confidence: 99%

Effects of non-B DNA sequences on transgene expression

Kamiya

Goto

Harashima

2009

Journal of Bioscience and Bioengineering

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Running title: Effects of non-B DNA sequences on transgene expression DNA conformation may be an important factor affecting gene transcription.In this study, we examined how DNA sequences with unusual conformations affect transgene expression. A 30 and (CG) 15 sequences that can adopt the B' and Z conformations, respectively, were introduced into a β-actin promoter. Luciferase plasmids containing the manipulated promoter were transfected into NIH3T3 cells by electroporation and were delivered into mouse livers with a hydrodynamics-based injection. Expression from plasmid with the (CG) 15 sequence was multiple times higher than expression from control plasmid DNA. The A 30 sequence also tended to enhance expression. These results suggest that non-B DNA sequences could improve transgene expression in cells.

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“…MUKHERJEE, S. N. SARKAR, P. RAYCHAUDHURI AND S. K. MAZUMDAR conformations [5,6]. This constantly exchanges energy and motion with solvent molecules; thus, internal motions within the structure are set up.…”

mentioning

confidence: 99%

Propagation of elastic waves in DNA

Mukherjee

Sarkar

Raychaudhuri

et al. 1983

International Journal of Mathematics and Mathematical Sciences

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Polymorphism of DNA double helices

Cited by 418 publications

References 26 publications

Raison d'être and structural model for the B‐Z transition of poly d(G‐C)^∗poly d(G‐C)

Raison d'être and structural model for the B‐Z transition of poly d(G‐C)^∗poly d(G‐C)

Effects of non-B DNA sequences on transgene expression

Propagation of elastic waves in DNA

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Polymorphism of DNA double helices

Cited by 418 publications

References 26 publications

Raison d'être and structural model for the B‐Z transition of poly d(G‐C)∗poly d(G‐C)

Raison d'être and structural model for the B‐Z transition of poly d(G‐C)∗poly d(G‐C)

Effects of non-B DNA sequences on transgene expression

Propagation of elastic waves in DNA

Contact Info

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Resources

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Raison d'être and structural model for the B‐Z transition of poly d(G‐C)^∗poly d(G‐C)

Raison d'être and structural model for the B‐Z transition of poly d(G‐C)^∗poly d(G‐C)