Polymorphism of COI gene in honey bees from different regions of Ukraine

Cherevatov, O. V.; Melnik, E. O.; Волков, Р. А.

doi:10.7124/visnyk.utgis.18.1-2.1351

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…Some of them pertained to studying the genomes as a whole or their parts in subspecies A. m. carnica, A. m. mellifera, which is significant as these bees inhabit the territory of Ukraine (Meixner et al, 2007), where such research is limited due to insufficient funding, lack of modern equipment, and shortage of scientific personnel. Despite these challenges, the Department of Molecular Genetics and Biotechnology at the Chernivtsi National University has been actively conducting research on mitochondrial DNA of bees (Cherevatov et al, 2019, Cherevatov et al, 2020. There are also several sporadic studies that, unfortunately, had not been continued (Metlytska et al, 2010;Metlytska et al, 2012;Hryhorchuk et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Features of determining the subspecies status of honey bees (Apis mellifera) based on morphometric wing indicators of drones

Yarovets,

Cherevatov,

Galatiuk

et al. 2024

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As of today, in Ukraine, there are no opportunities to conduct genetic research on a sufficient scale to identify the composition of the genomes of honey bees. Wing morphometrics of drones is a readily available alternative for obtaining information about the approximate composition of DNA. The goal of the work was to formulate the principles of the main stages of research and illustrate the procedure for obtaining morphometric data, methods of their processing, and possibilities for interpreting the obtained results. In the package of statistical data processing application programs Statistica, the classification of 2,147 drone wings obtained from 6 apiaries of Zhytomyr, Mykolaiv, and Kyiv regions was carried out according to eight indices: Ci, Dbi, Disc.sh, Pci, Ri, Ci.3, Ci.2.1, and Ci.2.2. We propose a method for obtaining morphometric wing standards of bees based on studying a significant number of bee colonies from various apiaries in specific regions of Ukraine. Using a two-stage methodology, three datasets were formed and recommended for use as standards: one for the local population of Ukrainian bees and two for populations of the A.m.mellifera subspecies distributed in the Polissia region. We analyzed the reliability of results in determining the taxonomic affiliation of bees was analyzed. Using the example of a single bee colony, a detailed procedure is demonstrated to identify taxonomic affiliation through utilization of the obtained standards. It has been demonstrated that in case of reliable identification of standards belonging to specific subspecies, it is possible to predict the likely composition of chromosomal sets in the genomes of drones, queens, and worker bees partially corresponding to the wing phenotype.

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Section: Introductionmentioning

confidence: 99%

Features of determining the subspecies status of honey bees (Apis mellifera) based on morphometric wing indicators of drones

Yarovets,

Cherevatov,

Galatiuk

et al. 2024

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“…Ukrainian steppe bee breed (Apis mellifera sossimai) is most widespread in Ukraine and occupies more than 70% of its territory. However, as a result of decades of hybridisation with other local breeds (carpathian (Apis mellifera carpatica) and polissian subspecies Apis mellifera mellifera), as well as with previously popular central russian (Apis mellifera mellifera), grey mountain caucasian (Apis mellifera caucasica) and popular now Italian (Apis mellifera carnica and Apis mellifera ligustica), as a result of the unconscious use by beekeepers of bee breeds that are not registered for Ukraine, the purity of the drone background of beekeeping farms is blurred, as a result of which the economically useful features have changed (Cherevatov et al, 2020). In addition, bees are susceptible to reduced genetic diversity because they are haplodiploid (Grozinger & Zayed, 2020).…”

Section: Introductionmentioning

confidence: 99%

Morphometric characteristics of Ukrainian steppe bees depending on the method of controlled queen mating

Saluyk¹

2023

Animal Science and Food Technology

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Bee breed is an important factor in determining bee family development and productivity, as it affects honey production, disease resistance, climate adaptation, and bee behaviour characteristics. The purpose of the study was to establish the relationship between the methods of mating queens and the morphological characteristics of bees of the Ukrainian steppe breed and the productive indicators of bee colonies. To conduct the research, 43 bee colonies with queen sisters of the Ukrainian steppe breed mated by different methods of natural mating were established in the Vinnytsia oblast. According to the results of mating, there were 22 queen bees in the control group, and 21 queen bees in the experimental group. The queens of the first control group mated in a normal natural way without any restrictions. The queen bees of the experimental group mated under time and space control. Based on the results of the research, the relationship between the integrity of the colony and its morphological features and productive indicators was established. Thus, experimental families have different degrees of belonging to the Ukrainian steppe breed, control – 59% and experimental – 87.2% (p < 0.001). According to the cubital index, the advantage was 8.2% (p < 0.001) in favour of the experimental group. The best family integrity on the hantel index was observed in families in which queens were mated in a controlled time and space, with a difference of 3.32% (p < 0.001). In the control group, where uncontrolled mating of queens was performed, more bees were found for discoid displacement with an excess of the standard indicator for the breed. Families in the experimental group were stronger by 14.5% (p < 0.001) compared to the control group. They provided 41.9% more honey, built 22.1% more honeycombs (p < 0.01), and the need for Kandy feeding was 31.8% less (p < 0.001). In addition, the bees of the experimental group had pronounced hygienic behaviour and required less labour costs. The practical significance of the study lies in the possibility of establishing patterns of action of factors on a particular breed and confirms the need to implement breeding programmes in beekeeping

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“…This region represents a steep gradient between mountainous remote areas with traditional village livelihoods, and lowlands with intensive farming, mainly rapeseed (Brassica napus), soybean (Glycine max), sunflower (Helianthus annuus) crops and apple orchards (Fedoriak et al, 2021). Honey bees, which are currently widespread on the territory of the Chernivtsi region, are mainly hybrids between two subspecies, Apis mellifera carnica and A. m. macedonica (Cherevatov et al, 2019;Cherevatov et al, 2020). Being the smallest region (8,100 km 2 ), it represents 1.3% of the total area of Ukraine.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical quality indicators of honey: An evaluation in a Ukrainian socioecological gradient

Zhuk¹,

Sytnikova²,

Fylypchuk³

et al. 2022

Regul. Mech. Biosyst.

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Physical and chemical quality indicators of 65 polyfloral honey samples from three administrative districts of Chernivtsi region in South Western Ukraine were studied. The chosen administrative districts were Putyla (representing the ‘Traditional villages’ stratum), Storozhynets (the ‘Intermediate’ stratum) and Khotyn (the ‘Intensive agriculture’ stratum), which reflect a steep gradient of social and ecological conditions such as land cover and land use, level of economic development, culture and demography. The quality of honey was determined in accordance with the requirements of the Ukrainian national standard and the EU Directive relating to honey (or Codex Alimentarius Honey Standard) by using the following indicators: reducing sugars and moisture content, diastase activity, free acidity, pH, electrical conductivity, hydroxymethylfurfural (HMF) and proline content. The profile of carbohydrates was analyzed, in particular glucose, fructose, sucrose, maltose, trehalose, melezitose, raffinose. The ratio of fructose to glucose (F/G) was determined. All tested samples complied with the international standards for the mass fraction of reducing sugars, diastase activity and hydroxymethylfurfural content. For physical and chemical parameters, the studied honey samples were of high quality. The content of the reducing sugars in the honey samples varied within the range of 66.0–97.6%. The fructose content ranged from 342 to 549 mg/g, and the glucose content variation ranged within 283–517 mg/g. The average fructose/glucose ratio was 1.2 for honey samples from the three studied districts. Besides fructose and glucose, some oligosaccharides, such as maltose, trehalose and melezitose, were found in the examined honey. Melezitose was detected in the honey samples from ‘Traditional village’ (21 samples) and ‘Intermediate’ (5 samples) districts. The total variability of HMF content in the studied 65 honey samples from apiaries in the three districts of Chernivtsi region ranged from 0.19 to 30.8 mg/kg. The minimum moisture content was found to be 16.2% (in the ‘Traditional village’ and ‘Intermediate’ strata), and 22.2% was the maximum (in the ‘Intermediate’ stratum). Our studies have shown that free acidity of the samples varied within the range 13.5 to 58.0 meq/kg. Proline content variability for the three studied geographical areas ranged from 82.3 to 1201.2 mg/kg. The studied samples of honey had a low pH level (~ 3.7), high content of proline (~ 513 mg/kg) and reducing sugars (~ 80%), which indicates its nutritional value and naturalness. Deviations from the honey standards in moisture content, acidity and electrical conductivity was revealed in 8% to 10% of all samples.

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Polymorphism of COI gene in honey bees from different regions of Ukraine

Cited by 5 publications

References 18 publications

Features of determining the subspecies status of honey bees (Apis mellifera) based on morphometric wing indicators of drones

Features of determining the subspecies status of honey bees (Apis mellifera) based on morphometric wing indicators of drones

Morphometric characteristics of Ukrainian steppe bees depending on the method of controlled queen mating

Physicochemical quality indicators of honey: An evaluation in a Ukrainian socioecological gradient

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