Polymorphism of acid and neutral .ALPHA.-glucosidases in banana pulp:Changes in apparent pIs and affinity to Con A of the enzymes during ripening.

Partial Purification and Characterization of Acid and Neutralα-Glucosidases from Preclimacteric Banana Pulp Tissues

“…29 ) Furthermore, AAG, but not NAG, in preclimacteric bananas was found to be mostly a glycoprotein that bound to a Con ASepharose column and the amount of the glycoprotein decreased upon ripening. 29 ) We also observed the presence of an ionically bound form of AAG in both ripe and unripe bananas, of which the apparent pI was different * To whom correspondence should be addressed. from that of the soluble form.…”

“…The temperature giving 50% residual activities of the AAG and NAGs were 72°C and 52°e, respectively, which were consistent with the previous results. 29 ) Table III shows the effects of various metal ions (1 mM) on the AAG and NAG activities. For the AAG, no or less than 5% inhibition was observed by Fe 2 +, Cu 2 +, Zn 2 +, Ca 2 +, Mg2+, and Cd 1 +, and the 10-15 % inhibition was by Se 2 +, Sb 2 +, Co 2 +, NF +, Li +, Pb 2 +, and Sr2 +.…”

Extraction of Soluble and Bound α-Glucosidases from Banana Pulp

“…The activities of AAG and NAG in these fractions were measured at pH 4.5 and pH 6.5, respectively, using 4-methylumbelliferyl-a-D-glucoside as a substrate, as reported previously. 3) Distribution of the soluble and bound a-glucosidases in green and overripe bananas is shown in Fig. I.…”