Polymorphism in ftsI gene and  -lactam susceptibility in Portuguese Haemophilus influenzae strains: clonal dissemination of  -lactamase-positive isolates with decreased susceptibility to amoxicillin/clavulanic acid

Barbosa, Andréa; Giufrè, Maria; Cerquetti, Marina; Bajanca-Lavado, Paula

doi:10.1093/jac/dkq533

Cited by 52 publications

(44 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In agreement with previous studies [11,12,13], gBLNAR is increasing at a constant rate in respiratory isolates of H. influenzae . The production of β-lactamase was not the main mechanism of β-lactam antibiotic resistance in this study.…”

Section: Discussionsupporting

confidence: 93%

Antimicrobial Resistance Genotype Trend and Its Association with Host Clinical Characteristics in Respiratory Isolates of Haemophilus influenzae

Hagiwara¹,

Baba²,

Shinohara³

et al. 2012

Chemotherapy

View full text Add to dashboard Cite

Background: β-Lactam resistance genotype trends in clinical isolates of Haemophilus influenzae and their correlation with the clinical background were analyzed. Methods: Five hundred and ten respiratory isolates of H. influenzae collected during the period 2002–2009 were classified by PCR into gBLNAS (genotype for β-lactamase-negative ampicillin-susceptible), gBLNAR (genotype for β-lactamase-negative ampicillin-resistant) and 3 other genotypes. The associations with host clinical data and antimicrobial susceptibility were analyzed in all 144 isolates between 2008 and 2009. Results: The 8-year trend analysis detected an increase in gBLNAR with a decrease in gBLNAS. The probability of being a causative pathogen did not differ between genotypes. Host clinical characteristics such as age and gender did not differ with gBLNAR or gBLNAS, but the underlying respiratory diseases did differ. gBLNAR was found at the highest rate in 83% of isolates from patients with nontuberculous mycobacteriosis. In contrast, gBLNAR accounted for as little as 33% of isolates from chronic obstructive pulmonary disease. Conclusions: There were no differences in the pathogenicity of gBLNAR and gBLNAS. The underlying respiratory diseases may be related to the resistance genotype.

show abstract

Section: Discussionsupporting

confidence: 93%

Antimicrobial Resistance Genotype Trend and Its Association with Host Clinical Characteristics in Respiratory Isolates of Haemophilus influenzae

Hagiwara¹,

Baba²,

Shinohara³

et al. 2012

Chemotherapy

View full text Add to dashboard Cite

show abstract

“…However, the Arg517 substitution was not found in either our BLNAR isolates or in previously described H. parainfluenzae isolates (García-Cobos et al, 2013;Tristram et al, 2008). The Val511Ala, Ala530Ser (both near the conserved KTG motif) and Thr574Ala changes have been reported previously in H. parainfluenzae linked to resistance or reduced susceptibility to b-lactams, although often in combination with Asn526Ser, and rarely in H. influenzae (Barbosa et al, 2011;García-Cobos et al, 2013;Kishii et al, 2010;Tinguely et al, 2013). Obviously, in our H. parainfluenzae isolates the possible presence of additional resistance mechanisms affecting susceptibility to b-lactams, such as overexpression of the AcrAB efflux pump or alterations in outer membrane permeability, cannot be ruled out (Kaczmarek et al, 2004).…”

Section: Discussionsupporting

confidence: 59%

Genital carriage of the genus Haemophilus in pregnancy: species distribution and antibiotic susceptibility

Cardines

Daprai

Giufrè

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

Recent reports have hypothesized that colonization of the maternal genital tract with noncapsulated Haemophilus influenzae could result in neonatal invasive disease. In this study, genital carriage of the genus Haemophilus was investigated in 510 pregnant women attending an Italian hospital for routine controls. Overall, vaginal carriage of the genus Haemophilus was 9.0 % (46/510). A high colonization rate with Haemophilus parainfluenzae (37/510, 7.3 %) was found; other species, such as Haemophilus pittmaniae (7/510, 1.4 %) and Haemophilus haemolyticus (2/510, 0.4 %), were detected for the first time in the genital flora by 16S rRNA gene sequencing. Notably, no H. influenzae was identified, in agreement with previous investigations indicating that this species is rarely isolated from the genito-urinary tract of pregnant women. No antibiotic resistance was detected in H. pittmaniae and H. haemolyticus, but quite a high degree of ampicillin (10/37, 27 %) and ciprofloxacin (3/37, 8.1 %) resistance was observed in H. parainfluenzae. Five ampicillin-resistant isolates were b-lactamase producers, whereas five isolates exhibited a b-lactamase-negative ampicillin-resistant (BLNAR) phenotype. Sequencing of penicillin-binding protein 3 revealed that Val511Ala, Asn526Ser, Ala530Ser and Thr574Ala changes were associated with BLNAR phenotypes. Two ciprofloxacin-resistant isolates carried substitutions in both GyrA (Ser84Phe and Asp88Tyr) and ParC (Ser84Tyr and Met198Leu); the other ciprofloxacin-resistant isolate had substitutions in ParC, only (Ser138Thr and Met198Leu). In conclusion, ,10 % of pregnant women carried a species of Haemophilus in their genital tract. The emergence of non-b-lactamase-mediated resistance in genital H. parainfluenzae is a matter of concern because of the risk of mother-tobaby transmission.

show abstract

“…12,20 b-Lactamase-producing strains (BLPAR) are prevalent globally 13 ; however, the proportion of BLNAR strains has increased since the first report in the early 1980s. 2,9,12,15,22,25,28 Additionally, the incidence of b-lactamase-producing strains with PBP3 mutations (BLPACR strains) that exhibit decreased susceptibility to amoxicillin/clavulanic acid is increasing. 2,6 In South Korea, NTHi strains consisted of 38.9% BLPAR, 29.3% BLNAR, and 8.3% BLPACR from 2000 to 2005.…”

Section: Introductionmentioning

confidence: 99%

“…2,9,12,15,22,25,28 Additionally, the incidence of b-lactamase-producing strains with PBP3 mutations (BLPACR strains) that exhibit decreased susceptibility to amoxicillin/clavulanic acid is increasing. 2,6 In South Korea, NTHi strains consisted of 38.9% BLPAR, 29.3% BLNAR, and 8.3% BLPACR from 2000 to 2005. 18 Another study of nationwide acute respiratory infection during 2005 and 2006 reported 47.2% BLPAR, 6.1% BLNAR, and 5.2% BLPACR strains among NTHi.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of theftsIGene in β-Lactamase-Nonproducing Ampicillin-Resistant and β-Lactamase-Producing Amoxicillin-/Clavulanic Acid-Resistant NasopharyngealHaemophilus influenzaeStrains Isolated from Children in South Korea

Park

Kim²,

Shin³

et al. 2013

Microbial Drug Resistance

View full text Add to dashboard Cite

Haemophilus influenzae frequently colonizes the nasopharynx of children and adults, which can lead to a variety of infections. We investigated H. influenzae carriage in the nasopharynx of 360 children, in terms of (1) the prevalence of strains with decreased susceptibility, and (2) the presence of amino acid substitutions in PBP3. One hundred twenty-three strains were isolated (34.2%, 123/360), 122 of which were classified as nontypable H. influenzae (NTHi). Of these, β-lactamase-nonproducing ampicillin-susceptible strains accounted for 26.2%, β-lactamase-producing-ampicillin-resistant strains for 9.0%, β-lactamase-nonproducing ampicillin-resistant (BLNAR) strains for 40.2%, and β-lactamase-producing amoxicillin-/clavulanic acid-resistant (BLPACR) for 24.6%, respectively. Pulsed field gel electrophoresis (PFGE) patterns were so diverse that they were clustered into 41 groups. The amino acid substitutions in the transpeptidase domain (292 amino acids) of ftsI in BLNAR isolates showed that group IIb accounted for 30.6%, IIc for 8.2%, IId for 16.3%, III for 32.7%, and the others for 12.2%. Moreover, groups IIb (56.7%; 17/30) and III (23.3%; 7/30) were prevalent among BLPACR strains. They were subclassified into more diverse sequence subtypes by analysis of the entire PBP3 (610 amino acids). Groups IIb, IIc, IId, and III exhibited 13, four, six, and four sequence subtypes, respectively. Such a genetic diversity is likely indicative of significant potential for decreased antimicrobial susceptibility in nasopharyngeal-colonizing NTHi strains.

show abstract

Polymorphism in ftsI gene and -lactam susceptibility in Portuguese Haemophilus influenzae strains: clonal dissemination of -lactamase-positive isolates with decreased susceptibility to amoxicillin/clavulanic acid

Abstract: This study shows that the non-enzymatic mechanism of resistance to β-lactams is widespread among H. influenzae isolates in Portugal. Clonal dissemination of BLPACR strains showing high resistance to ampicillin and reduced susceptibility to amoxicillin/clavulanic acid was documented.

Cited by 52 publications

References 33 publications

Antimicrobial Resistance Genotype Trend and Its Association with Host Clinical Characteristics in Respiratory Isolates of <b><i>Haemophilus influenzae</i></b>

Antimicrobial Resistance Genotype Trend and Its Association with Host Clinical Characteristics in Respiratory Isolates of <b><i>Haemophilus influenzae</i></b>

Genital carriage of the genus Haemophilus in pregnancy: species distribution and antibiotic susceptibility

Genetic Diversity of theftsIGene in β-Lactamase-Nonproducing Ampicillin-Resistant and β-Lactamase-Producing Amoxicillin-/Clavulanic Acid-Resistant NasopharyngealHaemophilus influenzaeStrains Isolated from Children in South Korea

Contact Info

Product

Resources

About