Polymorphism identification in the goat MSTN gene and association analysis with growth traits

An, Xiaopeng; Wang, J. G.; Hou, Jinxing; Zhao, Haibo; Bai, Liang; Li, G.; Wang, L. X.; Liu, X. Q.; Xiao, Wan-xia; Song, Yuxuan; Cao, Bin

doi:10.17221/4415-cjas

Cited by 11 publications

(6 citation statements)

References 29 publications

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“…The SSCP analysis of PCR products of primer pair 2 refers to An et al (2011) [17]. In addition, PCR products (5μl) of primer pair 2 were mixed with 1 μl 10 × buffer, 3 U Eco81Ⅰ(TaKaRa, Dalian, China) and 3.5 μl sterilized ddH2O, and then incubated for 1.5 h at 37°C.…”

Section: Snp Genotyping and Sequencingmentioning

confidence: 99%

Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

An¹,

Hou²,

Zhao³

et al. 2012

ENG

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In this study, polymorphisms of the DGAT1 and STAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic variants were identified: DQ380250: g.407_408insC, AJ237937: g.6798C>T and g.6852C>T in both breeds. At g.407_408insC locus, the frequencies of C 1 allele were 0.79-0.85, and frequencies of C 2 allele were 0.21-0.15. At g.6852C>T locus, frequencies of C 3 allele were 0.70-0.72, and frequencies of T 3 allele were 0.30-0.28. Compared with goats with C 1 C 1 and C 3 C 3 , those with C 1 C 2 and C 3 T 3 genotypes had significant effects on milk yield and fat percentage (P<0.05), respectively. The result showed that does with C 1 C 1 C 3 T 3 and C 1 C 2 C 3 T 3 had higher milk yield than those with C 1 C 2 C 3 C 3 (P < 0.05). In addition, the combined effect of C 1 C 2 C 3 T 3 on milk fat percentage was the highest in comparison with other combination genotypes (P<0.05).

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Section: Snp Genotyping and Sequencingmentioning

confidence: 99%

Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

An¹,

Hou²,

Zhao³

et al. 2012

ENG

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“…The present study in Marwari goat revealed low genetic diversity in exon 3 of MSTN gene which is in agreement with the similar study conducted in Saanen and Boer × Guanzhong goats by An et al . [ 27 ], who also observed one homozygote and a heterozygote genotypic pattern revealed by SSCP in exon 3 of caprine MSTN gene. However, a similar type of study in Sanjabi and Zel sheep by Soufy et al .…”

Section: Resultsmentioning

confidence: 97%

Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat

et al. 2016

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Aim:To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.Materials and Methods:A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer’s protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern.Results:The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively.Conclusion:Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan.

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“…Each 25 µL reaction contained 50 ng of sample DNA, 0.4 µM of each primer, 1X PCR buffer (10 mMTris-HCl, pH 8.0, 50 mM KCl), 2.0 mM MgCl2, 0.2 mM of each dNTP and 1 U of Taq DNA polymerase (Invitrogen). Amplification reactions were carried out in a thermocycler (Applied BioSystem), with 5 3 F-TCTTTAATAATGACTCCCTGCG R-GAACACCCACAGCGATCTACT [12] min denaturation at 95˚C, 35 cycles of 94˚C for 1 min, 59˚C for 1 min and a 72˚C extension for 1 min, and a final extension at 72˚C for 4 min. Sequencing was performed at Macrogen Inc. Korea, with an automated sequencer (Applied Biosystems) (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

Sequence Characterization of Coding Regions of the Myostatin Gene (GDF8) from Bakerwal Goats (<i>Capra hircus</i>) and Comparison with the Sheep (<i>Ovis aries</i>) Sequence

Ahad¹,

Beigh²,

Mehraj³

et al. 2016

OJAS

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The Bakerwal breed of goat in Kashmir valley is a good meat purpose breed of goat. That attains an appreciable body weight under a low input production system. As these goats are mainly reared by Gujjar and Bakarwal tribes of the J & K state, so they usually are fed with the weeds, herbs, shrubs and grasses of pastures that will otherwise go waste. These goats constitute the main livestock wealth. With the good productive and reproductive potential, it makes these animals an important animal protein source for developing countries like India. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although several alterations in the intron and exon regions have been identified. The objective of our work is to characterize the myostatin coding regions using gene sequencing and polymerase chain reaction methods of Capra hircus (Bakerwal breed) and to compare them with the Ovis aries and other livestock species of animal, looking for variations in nucleotide and protein sequences. As mutations in the myostatin gene can inactivate its expression and result in a non-functional protein, which leads to increase in muscle growth in many species. In this way, we * Corresponding author. W. A. Ahad et al. 158are able to identify 3 alterations on the presumed myostatin protein sequence as compared to non double-muscled ovine sequences.

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Polymorphism identification in the goat MSTN gene and association analysis with growth traits

Cited by 11 publications

References 29 publications

Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat

Sequence Characterization of Coding Regions of the Myostatin Gene (GDF8) from Bakerwal Goats (<i>Capra hircus</i>) and Comparison with the Sheep (<i>Ovis aries</i>) Sequence

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Polymorphism identification in the goat MSTN gene and association analysis with growth traits

Cited by 11 publications

References 29 publications

Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits

Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat

Sequence Characterization of Coding Regions of the Myostatin Gene (GDF8) from Bakerwal Goats (&lt;i&gt;Capra hircus&lt;/i&gt;) and Comparison with the Sheep (&lt;i&gt;Ovis aries&lt;/i&gt;) Sequence

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Sequence Characterization of Coding Regions of the Myostatin Gene (GDF8) from Bakerwal Goats (<i>Capra hircus</i>) and Comparison with the Sheep (<i>Ovis aries</i>) Sequence