Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits

Abstract

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C>T and g.6852C>T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C -allele were 0.79-0.85, and frequencies of C + allele were 0.21-0.15. At STAT5A g.6852C>T locus, frequencies of C allele were 0.70-0.72, and frequencies of T allele were 0.30-0.28. Compared with goats with DGAT1 C -C -, those with C -C + genotype had greater milk fat (P < 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype (P < 0.05). The results showed that does with C -C -CT and C -C + CT yielded more milk than those with C -C -CC (P < 0.05). In addition, does with C -C + CT had the highest milk fat in comparison with other combination genotypes (P < 0.05).

Analysis of A Sequence Nucleotide Polymorphism of STAT5A Gene in Garganica Goat Breed

“…The distribution of genotypes was in Hardy-Weinberg equilibrium (χ 2 =1.017, df=1, P=0.313). The lack of TT genotype was also reported by An et al [16] in Xinong Saanen and Guanzhong breeds. A very low frequency of TT individuals was subsequently reported by Wu et al [17] in Xinong Saanen and Hainan black populations.…”

“…The allelic frequencies found in Garganica goat breed were similar to those observed in Hainan black breed and, interestingly, the frequency of the C allele is higher if compared with the frequency of allele T in all the investigated samples. An et al [16] studied the association among genotypes at STAT5A/Eco81I locus and milk performance traits of animals: the does with CT genotype have greater milk yield than those carrying CC genotype (P<0.05). Moreover, Wu et al [17] found a positive relationship among STAT5A/Eco81I polymorphism and some phenotypic traits.…”

“…Nowadays, the Garganica goat breed is included in the list of Italian endangered breeds drawn up by the Italian Department for Environment, Food, and Rural Affairs (MiPAF, 2007). To date, only few polymorphisms of STAT5A gene were detected in goat species and few studies have been carried out on this concern [16], [17]. The aim of the present study was to investigate the STAT5A/Eco81I polymorphism in a sample of Garganica goat.…”

“…Genomic DNA was isolated from whole blood using ZR Genomic DNA II KitTM (Zymo Research). After genomic DNA isolation, all the samples were genotyped for STAT5A/Eco81I polymorphism by PCR-RFLP technique [16].…”

“…The aim of the present study was to investigate the STAT5A/Eco81I polymorphism in a sample of Garganica goat. This polymorphism is a substitution C→T at position 6852 within exon 7 [16]. Moreover, some genetic population indices were also reported.…”