Polymorphism, expression and structure analysis of key genes in the ovarian steroidogenesis pathway in sheep (<i>Ovis aries</i>)

Hu, Wenping; Liu, Mingqiu; Tian, Zhixi; Liu, Qiuyue; Zhang, Zhuangbiao; Tang, Jiao; He, Xiaoyun; Zhu, Yanyan; Wang, Yuanyuan

doi:10.1002/vms3.485

Cited by 9 publications

(5 citation statements)

References 49 publications

(63 reference statements)

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“…It is worth noting that the ovarian steroidogenesis pathway is closely related to ovarian function and development (Gareis et al., 2018), CYP11A1 is a protein‐coding gene, and cholesterol side‐chain cleavage enzyme involved in the biosynthesis of pregnenolone, a unique precursor for all important steroids, such as oestradiol‐17β (E2) (Chung et al., 1997). Movement of cholesterol and further conversion to pregnenolone are the presumed rate‐limiting steps of the steroidogenic pathway (Stocco, 2000), and CYP11A1 occupies a key regulatory part in the biosynthesis of steroids (Hu et al., 2021). STAR is expressed in the ovaries of a variety of animals, encoding steroidogenic acute regulatory protein, mainly overcoming the rate‐limiting transfer of cholesterol from the outer mitochondrial membrane to P450scc (encoded by CYP11A1) located in the cell membrane (Vallee, 2016; Yivgi‐Ohana et al., 2009).…”

Section: Discussionmentioning

confidence: 99%

“…are the presumed rate-limiting steps of the steroidogenic pathway (Stocco, 2000), and CYP11A1 occupies a key regulatory part in the biosynthesis of steroids (Hu et al, 2021). STAR is expressed in the ovaries of a variety of animals, encoding steroidogenic acute regulatory protein, mainly overcoming the rate-limiting transfer of distinct membrane protein that mediates cholesterol uptake from LDL by endocytosis (Jimenez et al, 2010;Parinaud et al, 1987) and plays central roles in lipoprotein metabolism, and Ldlr −/− female mice are subfertile (Azhar et al, 1998).…”

Section: Movement Of Cholesterol and Further Conversion To Pregnenolonementioning

confidence: 99%

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Transcriptome profiling of equine ovarian cortex during foetal and adult stages

Shen,

Davshilt,

Ren

et al. 2024

Reprod Domestic Animals

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The equine foetal ovary has some unique characteristics that differ from other animals, such as ovary hyperplasia and high oestrogen secretion, and inverted location of cortex and medulla present in adult mare. The ovarian cortex supports the process of folliculogenesis and development, the synthetics and expression of oestrogen; however, the character‐related molecular mechanisms and specific biological functions in equine foetal ovarian cortex are still poorly understood. To explore the associated regulatory networks and molecular events of equine foetal ovary during the hyperplasia stage, we analysed the transcriptomic differences between the equine second‐trimester foetal ovarian cortex and adult ovarian cortex by RNA‐seq. There were 6334 differentially expressed genes (DEGs) present in foetal vs. adult, of which 3234 and 3100 DEGs were upregulated and downregulated, respectively. GO and KEGG were used for functional and pathway enrichment analysis of the DEGs. In particular, we found that some DEGs with high fold changes were related to steroid production and metabolism, such as CYP11A1, CYP17A1, LDLR, STAR and SCARB1, which were in line with the efficient oestrogen production in the equine foetal ovary. The transcriptome profile revealed the functional and developmental characteristics and gene expression patten of the ovarian cortex in the foetal and adult stages of the mare. Our study provides new insights into the molecular mechanism of equine second‐trimester foetal ovarian development through transcriptome analysis and provides insights for improving care or treatment for the pregnant mare and the foetus.

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Section: Discussionmentioning

confidence: 99%

Section: Movement Of Cholesterol and Further Conversion To Pregnenolonementioning

confidence: 99%

Transcriptome profiling of equine ovarian cortex during foetal and adult stages

Shen,

Davshilt,

Ren

et al. 2024

Reprod Domestic Animals

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“…This process is promoted by increased expression of CYP11A1 (Chien et al, 2013). It is reported that the increased level of CYP11A1 promotes the development of antral and Graafian follicles (Gershon & Dekel, 2020), and the expression of CYP11A1 in ovarian tissue is far higher in multiparous Small Tail Han sheep than in uniparous Small Tail Han sheep (Hu et al, 2021). This observation is consistent with the results in the current study where we found that CYP11A1 was highly expressed in corpora lutea of polytocous sheep.…”

Section: Discussionmentioning

confidence: 99%

Differential expression and functional prediction of mRNA in the ovaries of Hanper sheep of high and low fecundity

Gao

et al. 2022

Reprod Domestic Animals

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Hanper ewes that were either monotocous or polytocous provided ovarian follicles of diameter >3 mm in the follicular phase and, in the luteal phase, samples of corpora lutea that had developed from follicles of diameter >3 mm. Differentially expressed mRNAs (monotocous versus polytocous) were then identified, and their functions were predicted. Results showed that 1508 mRNAs were differentially expressed in the follicular phase, with 885 being in the luteal tissues. Those which were differentially expressed in the follicular phase were mainly involved in the regulation of the ferroptosis and lysosome signalling pathways, whereas, for the luteal tissue, the differentially expressed mRNAs were mainly involved in the regulation of steroid biosynthesis. Based on the results, it was inferred that these pathways could explain variations in the fecundity of sheep.

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“…The prediction of the secondary structure of each protein and its variants was carried out using PredictProtein “ (accessed on 25 October 2022)”. SWISS-MODEL “ (accessed on 02 November 2022)” was used to predict the protein tertiary structure [ 29 ], and a phylogenetic tree was constructed using the neighbor-joining method in MEGA11 software [ 30 , 31 , 32 ].…”

Section: Methodsmentioning

confidence: 99%

Polymorphism, Expression, and Structure Analysis of a Key Gene ARNT in Sheep (Ovis aries)

Wang

Bao

et al. 2022

Biology

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Growth traits are influential factors that significantly affects the development of the sheep industry. A previous TMT proteomic analysis found that a key protein in the HIF signaling pathway, ARNT, may influence embryonic skeletal muscle growth and development in sheep. The purpose of this study was to better understand the association between the polymorphisms of ARNT and growth traits of sheep, and the potential function of ARNT. Real-time qPCR (qRT-PCR) of ARNT was carried out to compare its expression in different developmental stages of the muscle tissues and primary myoblasts in the Hu, Chinese merino, and Gangba sheep. The genetic variance of ARNT was detected using the Illumina Ovine SNP 50 K and 600 K BeadChip in the Hu and Ujimqin sheep populations, respectively. The CDS sequence of the ARNT gene was cloned in the Hu sheep using PCR technology. Finally, bioinformatic analytical methods were applied to characterize the genes and their hypothetical protein products. The qRT-PCR results showed that the ARNT gene was expressed significantly in the Chinese merino embryo after 85 gestation days (D85) (p < 0.05). Additionally, after the sheep were born, the expression of ARNT was significant at the weaning stage of the Hu sheep (p < 0.01). However, there was no difference in the Gangba sheep.In addition, six SNP loci were screened using 50 K and 600 K BeadChip. We found a significant association between rs413597480 A > G and the Hu sheep weight at weaning and backfat thickness in the 5-month-old sheep (p < 0.05), and four SNP loci (rs162298018 G > C, rs159644025 G > A, rs421351865 G > A, and rs401758103 A > G) were also associated with growth traits in the Ujimqin sheep (p < 0.05). Interestingly, we found that a G > C mutation at 1948 bp in the cloned ARNT CDS sequence of the Hu sheep was the same locus mutation as rs162298018 G > C identified using the 600 K BeadChip, which resulted in a nonconservative missense point mutation, leading to a change from proline to alanine and altering the number of DNA, protein-binding sites, and the α-helix of the ARNT protein. There was a strong linkage disequilibrium between rs162298018 G > C and rs159644025 G > A, and the ARNT protein was conserved among the goat, Hu sheep, and Texel sheep. And, we propose that a putative molecular marker for growth and development in sheep may be the G > C mutation at 1,948 bp in the CDS region of the ARNT gene. Our study systematically analyzed the expression, structure, and function of the ARNT gene and its encoded proteins in sheep. This provides a basis for future studies of the regulatory mechanisms of the ARNT gene.

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Polymorphism, expression and structure analysis of key genes in the ovarian steroidogenesis pathway in sheep (Ovis aries)

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 9 publications

References 49 publications

Transcriptome profiling of equine ovarian cortex during foetal and adult stages

Transcriptome profiling of equine ovarian cortex during foetal and adult stages

Differential expression and functional prediction of mRNA in the ovaries of Hanper sheep of high and low fecundity

Polymorphism, Expression, and Structure Analysis of a Key Gene ARNT in Sheep (Ovis aries)

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