Polymorphism and partial characterization of digestive enzymes in the spiny lobster Panulirus argus

Perera, Erick; Moyano, Francisco Javier; Díaz, Manuel; Perdomo-Morales, Rolando; Montero-Alejo, Vivian; Alonso, E.; Carrillo, Olimpia; Galich, Germán S.

doi:10.1016/j.cbpb.2008.03.009

Cited by 52 publications

(99 citation statements)

References 42 publications

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“…Our results differ from those shown in the hepatopancreas of the euryhaline crab Carcinus maenas, in which 2 amylase forms of about 30 kDa were reported by zymogram analysis (Van Wormhoudt et al, 1995). The differences in the number of amylase forms present in the hepatopancreas of crustaceans have been associated with variations in environmental and/or physiological factors (Borowsky, 1984;Le Moullac et al, 1997;Perera et al, 2008aPerera et al, , 2008bCastro et al, 2012). N. granulata behaves as a hyper/hyporegulator since it exhibits hemolymph osmolarity values higher and lower than those of the corresponding external medium upon acclimation to 6-10 psu and 37-60 psu, respectively, while it osmoconforms at 35 psu (Asaro et al, 2011;Pinoni et al, 2013;Michiels et al, 2015a;Pinoni et al, 2015; this work, Table).…”

Section: Discussioncontrasting

confidence: 98%

“…Several amylase forms have been found in the hepatopancreas of various decapod crustaceans, although with a high degree of interspecific variability. Moreover, the number of amylase forms present in the hepatopancreas can be influenced by internal and external factors in some species (Van Wormhoudt and Sellos, 2003;Perera et al, 2008aPerera et al, , 2008bCoccia et al, 2011;Le Moullec et al, 2011;Aragón-Axomulco et al, 2012;Rodríguez-Viera et al, 2016). To our knowledge, the work by Van Wormhoudt et al (1995) in Carcinus maenas represents the only available study on the occurrence of amylase forms in the hepatopancreas of a euryhaline crab.…”

Section: Introductionmentioning

confidence: 99%

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Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation of amylase in euryhaline crabs are lacking. We investigated the occurrence of amylase forms and the effect of acclimation to different salinities on their number and made a partial purification and characterization of the major form present in the hepatopancreas of Neohelice granulata. Zymogram analysis revealed 5 amylase forms in crabs acclimated to 35 psu (seawater) and 37 psu, and an additional band at 10 psu, but with a major form (29 kDa) in all cases, which was partially purified and characterized. Amylolytic activity was maximal between 30 and 40 °C; maintained at high NaCl concentrations (up to 4 M); increased by 5 mM K + , Li + , Co 2+ , and Mg 2+ (36%-45%); inhibited by Cu 2+ , Zn 2+ , Cd 2+ , Fe 2+ , and Mn 2+ (92.4% and 23.7%); not affected by Ni 2+ or Ba 2+ ; and enhanced almost 100% by Ca 2+ . Amylase exhibited Michaelis-Menten kinetics (starch: K m = 1.24 mg mL -1 ; glycogen: K m = 16.19 mg mL -1 ). The potential physiological significance and relationship to habitat conditions of the extra form in low salinity and the biochemical characteristics of the partially purified amylolytic activity (halotolerant, differential sensitivity to ions, capability to hydrolyze starch and glycogen) are discussed.

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Section: Discussioncontrasting

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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“…As in most crustacea, trypsin enzymes play a central role in protein digestion in spiny lobsters (Johnston, 2003;Celis-Gerrero et al, 2004;Perera et al, 2008a). Some information is available on the biochemical characterization of spiny lobster trypsins (Galgani and Nagayama, 1987;Celis-Gerrero et al, 2004;Perera et al, 2008a) and variation in trypsin activity throughout development and molt stages has also been reported (Johnston, 2003;Perera et al, 2008b).…”

Section: Introductionmentioning

confidence: 99%

“…Some information is available on the biochemical characterization of spiny lobster trypsins (Galgani and Nagayama, 1987;Celis-Gerrero et al, 2004;Perera et al, 2008a) and variation in trypsin activity throughout development and molt stages has also been reported (Johnston, 2003;Perera et al, 2008b). However, the effect of diet on these enzymes has been poorly studied and the time course of trypsin activity after ingestion of different diets has only recently been reported for one spiny lobster species (Jasus edwardsii) (Simon, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…In Panulirus argus, trypsin is a polymorphic enzyme (Perera et al, 2008a) and trypsin cDNA showing different expression rates has been cloned (Perera et al, 2010a). Additionally, trypsin isoforms in lobster appear to differ in catalytic properties or specificity as differences in digestion efficiency have been found among three distinct trypsin phenotypes (Perera et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

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Dietary protein quality differentially regulates trypsin enzymes at the secretion and transcription level in Panulirus argus by distinct signaling pathways

Perera

Rodríguez-Viera

Rodríguez-Casariego

et al. 2012

Journal of Experimental Biology

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with the results of previous digestibility studies (Ward et al., 2003; Irving and Williams, 2007;Perera et al., 2010b). Poor protein use could be related to some impairment in digestive progression when lobsters are fed on pelleted diets (Simon and Jeffs, 2008;Simon, 2009).The aim of the present work was to assess the effect of feeding P. argus with different protein sources on trypsin expression and secretion. Our results demonstrate that trypsin enzymes are differentially regulated at the transcription and secretion level by ingested proteins. Additionally, we provide evidence that intact proteins, rather than small peptides or free amino acids, are the major signal eliciting the prandial secretory response in lobster, whereas SUMMARYThe effects of pelleted diets with different protein composition (fish, squid or soybean meals as main protein sources) on trypsin secretion and expression were studied in the lobster Panulirus argus. Trypsin secretion was shown to be maximal 4h after ingestion. At this time, fish-and squid-based diets induced trypsin secretion, as well as up-regulation of the major trypsin isoform at the transcription level. While fish-and squid-based diets elicited a prandial response, soybean-based diet failed to stimulate the digestive gland to secrete trypsin into the gastric fluid or induce trypsin expression above the levels observed in fasting lobsters. In vitro assays showed that intact proteins rather than protein hydrolysates stimulate trypsin secretion in the lobster. However, the signal for trypsin transcription appears to be different to that for secretion and is probably mediated by the appearance of free amino acids in the digestive gland, suggesting a stepwise regulation of trypsin enzymes during digestion. We conclude that trypsin enzymes in P. argus are regulated at the transcription and secretion level by the quality of dietary proteins through two distinct signaling pathways. Our results indicate that protein digestion efficiency in spiny lobsters can be improved by selecting appropriated protein sources. However, other factors like the poor solubility of dietary proteins in dry diets could hamper further enhancement of digestion efficiency.

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Byproducts from Shellfish Harvesting and Processing

Klomklao

Poonsin

Benjakul

et al. 2019

Byproducts From Agriculture and Fisheries

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Polymorphism and partial characterization of digestive enzymes in the spiny lobster Panulirus argus

Cited by 52 publications

References 42 publications

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Dietary protein quality differentially regulates trypsin enzymes at the secretion and transcription level in Panulirus argus by distinct signaling pathways

Byproducts from Shellfish Harvesting and Processing

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