Polymorphism among RFL-PPR homologs in sunflower (Helianthus annuus L.) lines with varying ability for the suppression of the cytoplasmic male sterility phenotype

Anisimova, I. N.; Alpatieva, N. V.; Rozhkova, V. T.; Кузнецова, Е. Б.; Пинаев, А. Г.; Гаврилова, В. А.

doi:10.1134/s1022795414070023

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…The secondary structure of the SFA8 intron variants in the annual wild species H. agrophyllus was similar to the cultivated sunflower, but differed from that of the annual H. petiolaris and perennial H. giganteus L. Recently a possible role of the intron in the regulation of translation of SFA8 protein has been demonstrated (Sun et al, 2015). Probably the differences in the intron secondary structure may be important for understanding SFA8 expression pattern, and polymorphism of coding sequences can be useful for elucidating the molecular mechanisms of interspecific and intraspecific differences in the biochemical and functional properties of this valuable protein (Anisimova et al, 2018).…”

Section: Petiolarismentioning

confidence: 99%

The ideas of N.I. Vavilov and current problems of crop genetics

Anisimova

Radchenko

2020

BioComm

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Some results of the studies of plant genetic resources held in the VIR Department of Genetics are discussed in light of N. I. Vavilov’s theories of natural plant immunity, homologous series in hereditary variability, and interspecific hybridization. The long-term investigations resulted in identifying bread wheat, cultivated barley, oat, rye and sorghum accessions with new effective genes for resistance to diseases and pests. The large-scale immunological screening has revealed a narrow genetic diversity in wheat and barley for resistance to biotic and abiotic stressors. On the example of sunflowers, the significance of seed storage proteins for solving problems of domestication and introgressive hybridizations is demonstrated.

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Section: Petiolarismentioning

confidence: 99%

The ideas of N.I. Vavilov and current problems of crop genetics

Anisimova

Radchenko

2020

BioComm

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“…The absence of such markers in sunflower is explained by the lack of information about the nature of the Rf gene (genes). To identify the Rf1 gene, positional cloning method was used [19], and molecular markers based on polymorphic fragments of PPR genes have also been developed [20,21]. However, to this day, the sequence of the Rf1 gene remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Association Mapping of Fertility Restorer Gene for CMS PET1 in Sunflower

et al. 2019

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The phenomenon of cytoplasmic male sterility (CMS), consisting in the inability to produce functional pollen due to mutations in mitochondrial genome, has been described in more than 150 plant species. With the discovery of nuclear fertility restorer (Rf) genes capable of suppressing the CMS phenotype, it became possible to use the CMS-Rf genetic systems as the basis for practical utilization of heterosis effect in various crops. Seed production of sunflower hybrids all over the world is based on the extensive use of the PET1 CMS combined with the Rf1 gene. At the same time, data on Rf1 localization, sequence, and molecular basis for the CMS PET1 type restoration of fertility remain unknown. Searching for candidate genes of the Rf1 gene has great fundamental and practical value. Therefore, in this study, association mapping of fertility restorer gene for CMS PET1 in sunflower was performed. The genome-wide association study (GWAS) results made it possible to isolate a segment 7.72 Mb in length on chromosome 13, in which 21 candidates for Rf1 fertility restorer gene were identified, including 20 pentatricopeptide repeat (PPR)family genes and one Probable aldehyde dehydrogenase gene. The results will serve as a basis for further study of the genetic nature and molecular mechanisms of pollen fertility restoration in sunflower, as well as for further intensification of sunflower breeding.

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