Polymorphic variations in the ori sequences from the mitochondrial genomes of different wild-type yeast strains

Faugeron-Fonty, Godeleine; Goyon, Christophe

doi:10.1007/bf00365623

Cited by 10 publications

(3 citation statements)

References 37 publications

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“…Investigation of ori activity in wild-type strain. Most laboratory strains of S. cerevisiae have eight ori elements, with ori2, ori3, and oriS being active and oril, ori4, ori6, ori7, and ori8 being inactive (18,19). However, results from the crosses performed in this study could be interpreted to mean that oril is active in our wild-type strain.…”

Section: Resultsmentioning

confidence: 79%

Conversion at Large Intergenic Regions of Mitochondrial DNA in Saccharomyces cerevisiae

Skelly

Clark-Walker

1990

Molecular and Cellular Biology

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Saccharomyces cerevisiae mitochondrial DNA deletion mutants have been used to examine whether base-biased intergenic regions of the genome influence mitochondrial biogenesis. One strain (delta 5.0) lacks a 5-kilobase (kb) segment extending from the proline tRNA gene to the small rRNA gene that includes ori1, while a second strain (delta 3.7) is missing a 3.7-kb region between the genes for ATPase subunit 6 and glutamic acid tRNA that encompasses ori7 plus ori2. Growth of these strains on both fermentable and nonfermentable substrates does not differ from growth of the wild-type strain, indicating that the deletable regions of the genome do not play a direct role in the expression of mitochondrial genes. Examination of whether the 5- or 3.7-kb regions influence mitochondrial DNA transmission was undertaken by crossing strains and examining mitochondrial genotypes in zygotic colonies. In a cross between strain delta 5.0, harboring three active ori elements (ori2, ori3, and ori5), and strain delta 3.7, containing only two active ori elements (ori3 and ori5), there is a preferential recovery of the genome containing two active ori elements (37% of progeny) over that containing three active elements (20%). This unexpected result, suggesting that active ori elements do not influence transmission of respiratory-competent genomes, is interpreted to reflect a preferential conversion of the delta 5.0 genome to the wild type (41% of progeny). Supporting evidence for conversion over biased transmission is shown by preferential recovery of a nonparental genome in the progeny of a heterozygous cross in which both parental molecules can be identified by size polymorphisms.

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Section: Resultsmentioning

confidence: 79%

Conversion at Large Intergenic Regions of Mitochondrial DNA in Saccharomyces cerevisiae

Skelly

Clark-Walker

1990

Molecular and Cellular Biology

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“…Most laboratory strains of S. cerevisiae have eight ori elements, with ori2, ori3, and oriS being active and oril, ori4, ori6, ori7, and ori8 being inactive (18,19). However, results from the crosses performed in this study could be interpreted to mean that oril is active in our wild-type strain.…”

mentioning

confidence: 79%

Conversion at large intergenic regions of mitochondrial DNA in Saccharomyces cerevisiae.

Skelly

Clark-Walker

1990

Mol. Cell. Biol.

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Saccharomyces cerevisiae mitochondrial DNA deletion mutants have been used to examine whether base-biased intergenic regions of the genome influence mitochondrial biogenesis. One strain (A5.0) lacks a 5-kilobase (kb) segment extending from the proline tRNA gene to the small rRNA gene that includes oril, while a second strain (A3.7) is missing a 3.7-kb region between the genes for ATPase subunit 6 and glutamic acid tRNA that encompasses ori7 plus ori2. Growth of these strains on both fermentable and nonfermentable substrates does not differ from growth of the wild-type strain, indicating that the deletable regions of the genome do not play a direct role in the expression of mitochondrial genes. Examination of whether the 5-or 3.7-kb regions influence mitochondrial DNA transmission was undertaken by crossing strains and examining mitochondrial genotypes in zygotic colonies. In a cross between strain A5.0, harboring three active ori elements (ori2, ori3, and oriS), and strain A3.7, containing only two active oni elements (ori3 and oriS), there is a preferential recovery of the genome containing two active oni elements (37% of progeny) over that containing three active elements (20%). This unexpected result, suggesting that active oni elements do not influence transmission of respiratory-competent genomes, is interpreted to reflect a preferential conversion of the A5.0 genome to the wild type (41 % of progeny). Supporting evidence for conversion over biased transmission is shown by preferential recovery of a nonparental genome in the progeny of a heterozygous cross in which both parental molecules can be identified by size polymorphisms.

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“…Eight highly conserved ori sequences are found in the intergenic regions. The ori sequences consist of three GC rich clusters and four AT rich spacers and can be found with slight variations in all wild type (wt) strains [2,3]. One such ori sequence, oril is located between the pro tRNA and 15 S rRNA genes [4].…”

Section: Introductionmentioning

confidence: 99%

A 5 kb intergenic region containing oril in the mitochondrial DNA of Saccharomyces cerevisiae is dispensable for expression of the respiratory phenotype

Piškur

1988

FEBS Letters

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A mutant mitochondrial DNA of Saccharomyces cerevisiae strain R 0.54 has been shown to have a 5 kb deletion which removes the oril sequence and its surrounding intergenic sequences between the pro tRNA and 15 S rRNA genes. The deleted DNA has been sequenced and is found to be flanked by two 11 bp direct repeats. The deletion mutant displays a normal phenotype in non-fermentable media. This implies that the intergenic sequence is not required for expression of the respiratory phenotype.

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Polymorphic variations in the ori sequences from the mitochondrial genomes of different wild-type yeast strains

Cited by 10 publications

References 37 publications

Conversion at Large Intergenic Regions of Mitochondrial DNA in Saccharomyces cerevisiae

Conversion at Large Intergenic Regions of Mitochondrial DNA in Saccharomyces cerevisiae

Conversion at large intergenic regions of mitochondrial DNA in Saccharomyces cerevisiae.

A 5 kb intergenic region containing oril in the mitochondrial DNA of Saccharomyces cerevisiae is dispensable for expression of the respiratory phenotype

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