Polymorphic Modifications of Chitosan

Baklagina, Y. G.; Klechkovskaya, V. V.; Кононова, С. В.; Петрова, В. А.; Poshina, Daria N.; Orekhov, Anton S.; Skorik, Yury А.

doi:10.1134/s1063774518030033

Cited by 58 publications

(34 citation statements)

References 51 publications

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“…XRD analysis was performed to investigate the effect of RGEO introduction in the CS film crystallinity (Figure 4). CS film showed peaks at 2θ = 9.4, 19.8, and 20.9, which corresponded to the reflections 020, 200, and 220, respectively, of hydrated chitosan [77,[80][81][82]. The CS film with glycerol and without RGEO exhibited the highest crystallinity as compared to the other films.…”

Section: X-ray Diffractometry (Xrd) Analysis Of Cs+rgeo Filmsmentioning

confidence: 99%

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

et al. 2020

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The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world. Tissue engineering offers multiple options from biocompatible materials with easy resorption. Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications. In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions. An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films. Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO. In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation. Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v). The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials. On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation. However, mechanical properties demonstrated an understandable loss of tensile strength and Young’s modulus for F3 and F4. However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films. The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed. All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.

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Section: X-ray Diffractometry (Xrd) Analysis Of Cs+rgeo Filmsmentioning

confidence: 99%

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

et al. 2020

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“…The visible crystallinity (the presence of well-defined XRD patterns) is the effect of the stabilization of the structure and stiffening of the crystalline domains by hydrogen bonds and electrostatic interactions between the N-acetyl groups. Typically, two characteristic crystalline peaks at 10 • and 20 • of 2θ, with comparable crystallinity related to crystal I and crystal II, respectively, in the chitosan structure [54,55], are distinguished for chitosan, along with minor other reflections at higher 2θ values [56].…”

Section: Microstructural Characterization Of Ag/cs/silica Nanofibersmentioning

confidence: 99%

Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity

Zienkiewicz-Strzałka

Deryło–Marczewska

Skorik

et al. 2019

IJMS

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A simple, low-cost, and reproducible method for creating materials with even silver nanoparticles (AgNP) dispersion was established. Chitosan nanofibers with silica phase (CS/silica) were synthesized by an electrospinning technique to obtain highly porous 3D nanofiber scaffolds. Silver nanoparticles in the form of a well-dispersed metallic phase were synthesized in an external preparation step and embedded in the CS/silica nanofibers by deposition for obtaining chitosan nanofibers with silica phase decorated by silver nanoparticles (Ag/CS/silica). The antibacterial activity of investigated materials was tested using Gram-positive and Gram-negative bacteria. The results were compared with the properties of the nanocomposite without silver nanoparticles and a colloidal solution of AgNP. The minimum inhibitory concentration (MIC) of obtained AgNP against Staphylococcus aureus (S. aureus) ATCC25923 and Escherichia coli (E. coli) ATCC25922 was determined. The physicochemical characterization of Ag/CS/silica nanofibers using various analytical techniques, as well as the applicability of these techniques in the characterization of this type of nanocomposite, is presented. The resulting Ag/CS/silica nanocomposites (Ag/CS/silica nanofibers) were characterized by small angle X-ray scattering (SAXS), X-ray diffraction (XRD), and atomic force microscopy (AFM). The morphology of the AgNP in solution, both initial and extracted from composite, the properties of composites, the size, and crystallinity of the nanoparticles, and the characteristics of the chitosan fibers were determined by electron microscopy (SEM and TEM).

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“…The WAXD pattern of CS/DES-0 film prepared without ChCl demonstrated reflections at 2θ = 9.2 • (9.65 Å), 14.4 • (6.19 Å) and 19.6 • (4.59 Å). These peaks are typical for CS crystal structure in an anhydrous polymorph [42][43][44][45][46]. It has been considered that in the case of monocarboxylic acids the spontaneous water-removing from their chitosan salt occurs [47].…”

Section: Waxd Datamentioning

confidence: 95%

“…This is also in agreement with water sorption measurements, presented in Section 3.5., which demonstrate that the CS/DES-0 film has a lower water sorption ability than any sample containing NADES. The incorporation of 50 wt% NADES resulted in the change of peaks positions to 2θ = 8.42° (10.52 Å), 11.33° (7.84 Å) and 18.85° (4.77 Å) revealing that the introduction of 50 wt% NADES caused an emergence of hydrated polymorph of CS [44,46,48,49]. A further increase in the NADES content up to 67 wt% leads to the separation of CS chains due to the formation of NADES inside a film and to amorphization of the samples.…”

Section: Waxd Datamentioning

confidence: 97%

Ionic Conductivity and Structure of Chitosan Films Modified with Lactic Acid-Choline Chloride NADES

et al. 2020

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The natural deep eutectic solvent (NADES) based on choline chloride (ChCl) and lactic acid (LA) was used for the preparation of chitosan (CS) films by the solution casting method. The content of NADES in films was from 0 to 82 wt%. The impact of NADES on the morphology and crystalline structure of films was investigated using scanning electron microscopy as well as wide-angle and small-angle X-ray scattering. The experimental results allow to propose CS chains swelling in NADES. FTIR spectroscopy confirms the interactions between CS and NADES components via the formation of hydrogen and ion bonds. The thermal properties of the composite films were studied by simultaneous thermogravimetric and differential thermal analysis. Thermomechanical analysis demonstrated appearance of two transitions at temperatures between −23 and −5 °C and 54–102 °C depending on NADES content. It was found that electrical conductivity of film with 82 wt% of NADES reaches 1.7 mS/cm. The influence of the composition and structure of films on the charge carriers concentration and their mobility is discussed.

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Polymorphic Modifications of Chitosan

Cited by 58 publications

References 51 publications

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

Silver Nanoparticles on Chitosan/Silica Nanofibers: Characterization and Antibacterial Activity

Ionic Conductivity and Structure of Chitosan Films Modified with Lactic Acid-Choline Chloride NADES

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