Polymorphic microsatellite DNA markers for the Florida manatee (<i>Trichechus manatus latirostris</i>)

Pause, Kimberly C.; Nourisson, Coralie; Clark, Ann-Marie; Kellogg, Margaret E.; Bonde, Robert K.; McGuire, Patricia

doi:10.1111/j.1471-8286.2007.01781.x

Cited by 16 publications

(20 citation statements)

References 5 publications

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“…Amplifications were performed in a total volume of 15 lL, with 10 ng target DNA, 19 Sigma PCR Buffer (10 mM TrisHCl, pH 8. Pause et al 2007). For fragment analysis, the forward primers were labeled with the fluorescent dyes HEX or 6-FAM for processing and visualization on an ABI 3730xl Automated DNA Analyzer.…”

Section: Microsatellite Dna Amplification and Fragment Analysismentioning

confidence: 93%

Evidence of two genetic clusters of manatees with low genetic diversity in Mexico and implications for their conservation

et al. 2011

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The Antillean manatee (Trichechus manatus manatus) occupies the tropical coastal waters of the Greater Antilles and Caribbean, extending from Mexico along Central and South America to Brazil. Historically, manatees were abundant in Mexico, but hunting during the pre-Columbian period, the Spanish colonization and throughout the history of Mexico, has resulted in the significantly reduced population occupying Mexico today. The genetic structure, using microsatellites, shows the presence of two populations in Mexico: the Gulf of Mexico (GMx) and Chetumal Bay (ChB) on the Caribbean coast, with a zone of admixture in between. Both populations show low genetic diversity (GMx: N(A) = 2.69; H(E) = 0.41 and ChB: N(A) = 3.0; H(E) = 0.46). The lower genetic diversity found in the GMx, the largest manatee population in Mexico, is probably due to a combination of a founder effect, as this is the northern range of the sub-species of T. m. manatus, and a bottleneck event. The greater genetic diversity observed along the Caribbean coast, which also has the smallest estimated number of individuals, is possibly due to manatees that come from the GMx and Belize. There is evidence to support limited or unidirectional gene flow between these two important areas. The analyses presented here also suggest minimal evidence of a handful of individual migrants possibly between Florida and Mexico. To address management issues we suggest considering two distinct genetic populations in Mexico, one along the Caribbean coast and one in the riverine systems connected to the GMx.

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Section: Microsatellite Dna Amplification and Fragment Analysismentioning

confidence: 93%

Evidence of two genetic clusters of manatees with low genetic diversity in Mexico and implications for their conservation

et al. 2011

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“…Fragment analysis of 15 polymorphic microsatellite loci ( TmaKB60, TmaA02, TmaE01, TmaE02, TmaE07, TmaE08, TmaE11, TmaE26, TmaH13, TmaJ02, TmaK01, TmaF14, TmaM79, TmaSC5, TmaSC13 ) [16], [17], was achieved following Kellogg [28] PCR conditions, and visualized on an Applied Biosystems ABI 3100 Genetic Analyzer (Universidad de los Andes). All individuals were genotyped for at least 12 loci.…”

Section: Methodsmentioning

confidence: 99%

“…Using multiple unlinked loci for populations with smaller sampling sizes improves accuracy in population genetics estimates [13], [14], [15], and provides a solution to study population dynamics in manatee populations. Primers for amplification of highly polymorphic bi-parentally inherited nuclear microsatellite loci have been developed for the Florida manatee [16], [17], [18], and effectively used to study manatee population structure in Florida, Puerto Rico, Belize and Mexico [19], [20], [21].…”

Section: Introductionmentioning

confidence: 99%

Phylogeography and Sex-Biased Dispersal across Riverine Manatee Populations (Trichechus inunguis and Trichechus manatus) in South America

et al. 2012

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Phylogeographic patterns and sex-biased dispersal were studied in riverine populations of West Indian (Trichechus manatus) and Amazonian manatees (T. inunguis) in South America, using 410bp D-loop (Control Region, Mitochondrial DNA) sequences and 15 nuclear microsatellite loci. This multi-locus approach was key to disentangle complex patterns of gene flow among populations. D-loop analyses revealed population structuring among all Colombian rivers for T. manatus, while microsatellite data suggested no structure. Two main populations of T. inunguis separating the Colombian and Peruvian Amazon were supported by analysis of the D-loop and microsatellite data. Overall, we provide molecular evidence for differences in dispersal patterns between sexes, demonstrating male-biased gene flow dispersal in riverine manatees. These results are in contrast with previously reported levels of population structure shown by microsatellite data in marine manatee populations, revealing low habitat restrictions to gene flow in riverine habitats, and more significant dispersal limitations for males in marine environments.

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“…A panel of 16 polymorphic microsatellite primers (Garc´ıa-Rodr´ıguez et al, 2000;Pause et al, 2007) was PCR amplified using 118 individuals. The study included 88 individuals from the SLS system (12 Northern Lagoon and 76 Southern Lagoon), 21 from BCC, two from PL and seven amplifiable carcass samples.…”

Section: Microsatellite Dna Analysismentioning

confidence: 99%

Low genetic variation and evidence of limited dispersal in the regionally important Belize manatee

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Auil-Gomez

Tucker

et al. 2010

Animal Conservation

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The Antillean subspecies of the West Indian manatee Trichechus manatus is found throughout Central and South America and the Caribbean. Because of severe hunting pressure during the 17th through 19th centuries, only small populations of the once widespread aquatic mammal remain. Fortunately, protections in Belize reduced hunting in the 1930s and allowed the country's manatee population to become the largest breeding population in the Wider Caribbean. However, increasing and emerging anthropogenic threats such as coastal development, pollution, watercraft collision and net entanglement represent challenges to this ecologically important population. To inform conservation and management decisions, a comprehensive molecular investigation of the genetic diversity, relatedness and population structure of the Belize manatee population was conducted using mitochondrial and microsatellite DNA. Compared with other mammal populations, a low degree of genetic diversity was detected (H E = 0.455; N A = 3.4), corresponding to the small population size and long-term exploitation. Manatees from the Belize City Cayes and Southern Lagoon system were genetically different, with microsatellite and mitochondrial F ST values of 0.029 and 0.078, respectively (P 0.05). This, along with the distinct habitats and threats, indicates that separate protection of these two groups would best preserve the region's diversity. The Belize population and Florida subspecies appear to be unrelated with microsatellite and mitochondrial F ST values of 0.141 and 0.63, respectively (P 0.001), supporting the subspecies designations and suggesting low vagility throughout the northern Caribbean habitat. Further monitoring and protection may allow an increase in the Belize manatee genetic diversity and population size. A large and expanding Belize population could potentially assist in the recovery of other threatened or functionally extinct Central American Antillean manatee populations.

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Polymorphic microsatellite DNA markers for the Florida manatee (Trichechus manatus latirostris)

Cited by 16 publications

References 5 publications

Evidence of two genetic clusters of manatees with low genetic diversity in Mexico and implications for their conservation

Evidence of two genetic clusters of manatees with low genetic diversity in Mexico and implications for their conservation

Phylogeography and Sex-Biased Dispersal across Riverine Manatee Populations (Trichechus inunguis and Trichechus manatus) in South America

Low genetic variation and evidence of limited dispersal in the regionally important Belize manatee

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