Polymicrobic infection of hybrid striped bass (<i>Morone chrysops × Morone saxatilis</i>) with three bacterial pathogens: a case report

Pr, Bowser; Ga, Wooster; Cy, Chen; Rs, Mo

doi:10.1111/j.1365-2761.2003.00517.x

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…These changes and damage to the hepatic cells could have occurred due to the poisons released by the pathogenic bacteria during infection and the long‐term action of poisonous compounds and their metabolites on the liver cells. This suggestion concurs with the observations of Bowser, Wooster, Chen and Mo (2004), who reported that hybrid striped bass had necrotic foci in the liver when it was infected with Aeromonas salmonicida, Mycobacterium sp . and Vibrio anguillarum .…”

Section: Discussionsupporting

confidence: 93%

“…2b). Kidney cell damage due to pathogenic bacterial infection such as multiple necrotic foci in hybrid striped bass, Morone chrysops Morone saxatilis (Bowser et al 2004), congested kidneys in silver pomfret Pampus argenteus , Euphrasen (Duremdez, Al‐marzouk, Qasem, Al‐harbi & Gharabally 2004), haemorrhaging and necrosis in turbot Scophthalmus maximus (Björnsdóttir, Gudmundsdóttir, Bambir & Gudmundsdóttir 2005; Padr, Zarza, Dopazo, Cuadrado & Crespo 2006), lesions, enlarged kidneys and necrosis in rainbow trout (Hirvelä‐Koski, Pohjanvirta, Koski & Sukura 2006; Altinok, Balta, Capkin & Kayis 2007) and embolism in capillary and lymphatic vessels in Seriola dumerili (Hagiwara, Takano, Noguchi & Narita 2009) have also been reported previously.…”

Section: Discussionmentioning

confidence: 58%

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Evaluating the use of Lactobacillus acidophilus as a biocontrol agent against common pathogenic bacteria and the effects on the haematology parameters and histopathology in African catfish Clarias gariepinus juveniles

Al-Dohail

Hashim

Aliyu‐Paiko

2010

Aquaculture Research

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This study was carried out to evaluate the use of Lactobacillus acidophilus as a biocontrol agent against some common fish pathogenic bacteria (Staphylococcus xylosus, Aeromonas hydrophila gr.2 and Streptococcus agalactiae) in African catfish, Clarias gariepinus. Eight treatments were designed inclusive of 10 C. gariepinus juveniles (mean weight 190 g) per tank, each in triplicate. Four groups of fish were fed a diet supplemented with L. acidophilus, comprising about 3.01 × 107 colony‐forming units per gram of diet (the probiotics diet), while the other four groups were fed a diet not supplemented with probiotics (the non‐probiotics diet). In the first group, fish were injected with 1 mL physiological saline and fed the non‐probiotic diet (non‐probiotic control); in the second, third and fourth groups, fish were injected with 1 mL each of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the non‐probiotic diet (designated as non‐probiotic treatments; NPsx, NPah and NPsa respectively). In the fifth group, fish were injected with 1 mL physiological saline but fed the probiotic diet (probiotic control), while fish in the sixth, seventh and eighth groups were each injected with 1 mL of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the probiotic diet (and designated as probiotic treatments; Psx, Pah and Psa respectively). Blood samples were collected for haematology analysis, while samples of the liver and kidney were examined for pathohistology after 7 and 21 days of infection. The results showed that the haematology parameters, packed cell volume, haemoglobin, erythrocyte sedimentation rate, red blood cell, white blood cell, total serum protein, Mg2+, Ca2+, Cl−, glucose, cholesterol and total immunoglobulin concentrations and the pathohistology of the liver and kidney were better in the challenged fish (infected) maintained on the probiotic diet than those in the groups fed the non‐probiotic diet. It is concluded, based on these results, that L. acidophilus is useful as a probiotic agent in C. gariepinus against these pathogenic bacteria (S. xylosus, A. hydrophila gr.2 and S. agalactiae).

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 58%

Evaluating the use of Lactobacillus acidophilus as a biocontrol agent against common pathogenic bacteria and the effects on the haematology parameters and histopathology in African catfish Clarias gariepinus juveniles

Al-Dohail

Hashim

Aliyu‐Paiko

2010

Aquaculture Research

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“…Furthermore, HSB reared in recirculating aquaculture systems seem to have increased susceptibility to Gram-positive bacterial infections (Stoffregen et al 1996) including Mycobacterium sp. (Bowser et al 2004). Systemic infection by M. marinum can produce severe disseminated disease in commercial HSB operations that, in the absence of approved products for the treatment or prevention of this disease, can cause widespread mortality and economic loss.…”

Section: Introductionmentioning

confidence: 99%

Biochemical, molecular, and virulence characteristics of select Mycobacterium marinum isolates in hybrid striped bass Morone chrysops × M. saxatilis and zebrafish Danio rerio

Ostland

Watral²,

Whipps³

et al. 2008

Dis. Aquat. Org.

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A panel of 15 Mycobacterium marinum isolates was characterized by biochemical tests, sequencing the ribosomal DNA intergenic spacer (ITS) region and the heat shock protein 65 gene (hsp65) and pulsed-field gel electrophoresis (PFGE). The biochemical characteristics of all isolates were similar, except for Tween 80 hydrolysis. DNA sequence of hsp65 for a subset of isolates were identical; however, at position 5 of the ITS rDNA, a single nucleotide polymorphism was identified.Isolates possessing a guanine residue at this position (G strains) were unable to hydrolyze Tween 80, while isolates that contained an adenine residue at this position (A strains) were positive for Tween 80 hydrolysis. PFGE successfully discriminated between the G and A strains; all G strains had identical AseI restriction enzyme-cutting patterns while the A strains exhibited a variety of cutting patterns. Eight isolates (4 G and 4 A strains) were further characterized for virulence by experimental infection of hybrid striped bass (HSB) Morone chrysops × M. saxatilis and zebrafish Danio rerio. Seven of the 8 strains produced cumulative mortality ranging from 13.3 to 83.3% in the HSB virulence trial. The M. marinum reference strain ATCC 927 T did not produce mortality in HSB. HSB exposed to the G strains had significantly higher cumulative mortality than those exposed to the A strains. When these same isolates were tested in zebrafish, 6 of the 8 strains caused 100% cumulative mortality, with 2 of the A strains being the most pathogenic. In zebrafish, however, ATCC 927 T was virulent and produced 28.5% mortality. Collectively, we conclude that the M. marinum G strains are unique and may represent a distinct virulence phenotype in HSB, but this trend was not consistent in zebrafish. KEY WORDS: Mycobacterium marinum · Hybrid striped bass · Zebrafish · Mycobacteriosis · VirulenceResale or republication not permitted without written consent of the publisher

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Polymicrobic infection of hybrid striped bass (Morone chrysops × Morone saxatilis) with three bacterial pathogens: a case report

Cited by 2 publications

References 9 publications

Evaluating the use of Lactobacillus acidophilus as a biocontrol agent against common pathogenic bacteria and the effects on the haematology parameters and histopathology in African catfish Clarias gariepinus juveniles

Evaluating the use of Lactobacillus acidophilus as a biocontrol agent against common pathogenic bacteria and the effects on the haematology parameters and histopathology in African catfish Clarias gariepinus juveniles

Biochemical, molecular, and virulence characteristics of select Mycobacterium marinum isolates in hybrid striped bass Morone chrysops × M. saxatilis and zebrafish Danio rerio

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