Polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique

Mukherjee, A. S.; Pramanik, Suman; Das, Dipankar; Roy, Rupak; Therese, K Lily

doi:10.4103/0255-0857.136593

Cited by 8 publications

(2 citation statements)

References 5 publications

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“…Surgical treatments were observed in 50% of this case series, which agrees with other studies that reported it in approximately 30% to 73% of cases (12,15,16,27,41). A de nitive initial surgical procedure should be considered in any patient with strong clinical evidence of severe or refractory CPE, or when the clinical features are suggestive or microbiologically proven aggressive microorganisms, such as fungal infection, Onchobactrum Anthropi, P.Acnes, or polymicrobial infection (15,22,26,34,45,52,(72)(73)(74)77).…”

Section: Discussionmentioning

confidence: 99%

Chronic Pseudophakic Endophthalmitis After Uneventful Phakoemulsification: A Case Series and Review of Litterature

Mili¹,

Braham

Kaouel³

et al. 2021

Preprint

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Purpose: To report a retrospective case series of patients with chronic post operative endophthalmitis (CPE) after uneventful phacoemulsification (PKE) with intraocular lens (IOL) implantation. Methods: this study was conducted between January 2011 and June 2020, including patients with delayed-onset endopthalmitis occurring at least 2 weeks after uneventful PKE+IOL. Diagnosis was based on typical clinical features associated or not to proven sample culture. Treatment was based on step-by-step management, depending on results of microbiology and response of treatment. It consisted in first line medical treatment with a two-approach intraocular and systemic antiobiotics, followed, if necessary, by a second line conservative-IOL surgical treatment with pars plana vitrectomy (PPV), and a third line non-conservative-IOL surgical treatment with re-PPV associated to IOL explantation. Results: Seven patients were included with a mean age of 62,5 years. Mean duration interval between cataract surgery and diagnosis of CPE was 31,3 weeks. All patients presented a decrease of visual acuity with white intracapsular plaques. Only one patient had positive IOL culture. Medical treatment was sufficient in three cases. In the four other cases, PPV with IOL explantation and total capsular bag removal was conducted. Mean final BCVA was 20/160 with a gain of 4 lines and was ≥ 20/40 in 4 cases. Conclusion: The diagnosis of CPE is still challenging especially for difficulties in isolating microorganisms. It should always be considered in cases of recurrent ocular inflammation resistant to conventional treatment in operated eyes. Non-conservative IOL surgical treatment may be directly necessary in severe cases.

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Section: Discussionmentioning

confidence: 99%

Chronic Pseudophakic Endophthalmitis After Uneventful Phakoemulsification: A Case Series and Review of Litterature

Mili¹,

Braham

Kaouel³

et al. 2021

Preprint

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“…Although Kluyveromyces marxianus is not usually a pathogen for human disease, it has been recovered from catheters, associated with canditdemia, and found in biofilms on many indwelling deovices such as prosthetic heart valves and pacemakers. K. marxianus has been seen in about 1% to 3% of cases with female genital infections, surgical wounds, and infections related to upper respiratory tracts [2,3].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Properties of Silver Nanoparticles Synthe‌sized From Capparis Spinosa Fruit

et al. 2019

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Background: Nanoparticles (NPs) are colloidal systems with particles ranging from 10 to 100 nm in diameter. Because of their large surface-volume ratio, NPs are biologically active materials that could interact with biomolecules and microorganisms, enter into the cells, and affect the metabolic functions. The study aimed to biosynthesize silver nanoparticles (Ag-NPs) from Capparis spinosa fruit aqueous extract, and evaluate their Ag nanostructure characterization and antifungal activity.Materials and Methods: Capparis spinosa fruit aqueous was prepared with the percolation method. Then, silver NPs were synthesized using 0.01 M silver nitrate solution, and their formation was validated by color changing of the solution from green to dark brown . The NPs were purified using centrifugation and then dried in an oven for further analyses. Ag-NPs nanostructure characterization was determined by various techniques such as Fourier Transforms Infrared (FTIR) spectroscopy, Scanning Electron Microscope (SEM), and Ultraviolet-visible (UV-Vis) spectroscopy. Antifungal activity of Ag-NPs against three pathogenic fungi of Candida albicans, Candida glabrata, and Kluyveromyces marxianus was also evaluated using the microdilution method.Results: Synthesis of Ag-NPs from aqueous extract of C. spinosa fruit was done successfully. UV-Vis spectrum of Ag-NPs showed an absorbance peak around 420 nm, revealing Ag-NPs surface plasmon resonance (K max ). FTIR analysis showed that functional groups correspond to plant bioactive components, promoting the formation of Ag-NPs. Furthermore, spherical uniformity of the synthesized Ag-NPs from plant extract was confirmed by SEM analysis within the 50-80 nm size range. Our results showed that the produced Ag-NPs were spherical and in a suitable form and size (50-80 nm). The biosynthesized Ag-NPs had an inhibitory effect against all tested fungi with the minimum inhibitory concentration of 2500, 5000, and 625 µg/mL and minimum bactericidal concentration of 10000, 10000, and 156.25 µg/mL for C. albicans, C. glabrata, and K. marxianus, respectively. Conclusion:According to the UV-Vis spectrum, FTIR, and SEM results, we succeeded in synthesizing Ag-NPs from C. spinosa fruit aqueous extract. This research was the first report of Ag-NPs synthesized from aqueous extract of C. spinosa fruit. Our simple, quick, and inexpensive method for biosynthesis of a nanoparticle, which showed antifungal activity, provides a new potential antifungal agent for therapeutic applications.

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Chronic Endophthalmitis Masquerading as Uveitis

et al. 2016

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Polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique

Cited by 8 publications

References 5 publications

Chronic Pseudophakic Endophthalmitis After Uneventful Phakoemulsification: A Case Series and Review of Litterature

Chronic Pseudophakic Endophthalmitis After Uneventful Phakoemulsification: A Case Series and Review of Litterature

Antifungal Properties of Silver Nanoparticles Synthe‌sized From Capparis Spinosa Fruit

Chronic Endophthalmitis Masquerading as Uveitis

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