Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies

Jungbauer, Alois; Hahn, Rainer

doi:10.1016/j.chroma.2007.12.087

Cited by 203 publications

(165 citation statements)

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“…Another main advantage of monoliths is their flow-independent DBC allowing straight-forward scalability [3,[26][27][28]. A direct comparison between monoliths and particle-based resins for the purification of virus-like particles from yeast homogenate has been conducted by Burden et al The authors concluded that the monoliths are three-fold superior in terms of DBC with equivalent recovery in yield (90%) compared to particle-based resins [29].…”

Section: Adsorption Area and Dynamic Binding Capacitymentioning

confidence: 99%

“…Monolithic columns have a low absolute surface area, but a high adsorption area due to their porous structure [3], allowing a high dynamic binding capacity (DBC). Another main advantage of monoliths is their flow-independent DBC allowing straight-forward scalability [3,[26][27][28].…”

Section: Adsorption Area and Dynamic Binding Capacitymentioning

confidence: 99%

“…This can be critical for unstable products, which is why faster purification methods are needed. In the past twenty years, monolithic columns have become popular as an efficient tool for the purification of large biomolecules due to their high mass transfer properties and short purification times [3]. Monoliths are a special type of chromatographic column having a single block of a homogenous stationary phase with many interconnected channels [4].…”

Section: Introductionmentioning

confidence: 99%

“…The stationary phase of the monolith can be of various chemistries, allowing the purification of different kinds of biomolecules with different characteristics. Monoliths are the fourth generation chromatography material [3], succeeding beads, porous particle-based columns and membrane adsorbers. In the last two decades, numerous articles describing preparation, properties and applications of monolithic columns were published [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Monoliths in Bioprocess Technology

2015

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Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a 'yellow pages' for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.

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Section: Adsorption Area and Dynamic Binding Capacitymentioning

confidence: 99%

Section: Adsorption Area and Dynamic Binding Capacitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monoliths in Bioprocess Technology

2015

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“…These PolyHIPE have been found a variety of applications including catalytic supports [2], chromatographic separation [3,4], scaffolds for tissue engineering [5], etc. Pickering emulsion is stabilized by nanometer-or micrometer-sized particles 6 .…”

Section: Introductionmentioning

confidence: 99%

Interconnected Porous Material Prepared Via High Internal Phase Emulsion Stabilized by Mixture of Fe₃O₄and Tween85

Yang

Zhang

et al. 2016

MATEC Web Conf.

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Abstract.PolyHIPE monoliths with open-cell structure were prepared using an oil-in-water Pickering high internal phase emulsion (HIPEs) template. Fe 3 O 4 nanoparticles and Tween 85 were used to stabilize the HIPE. The effects of surfactant concentration, nanoparticle amount and internal phase fraction on the average void and interconnecting pore diameter and interconnectivity degree were investigated. Efficiency of these PolyHIPEs as catalyst for Fenton reaction to decompose methyl orange was tested. The results showed that the PolyHIPE was an excellent and reusable supporter for Fenton reaction.

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Membrane Chromatography

Pieracci

Thömmes

2010

Encyclopedia of Industrial Biotechnology

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It has been nearly 20 years since membrane chromatography was first introduced as a technology capable of purifying proteins. In that time, a large body of work has been generated which has described the fundamental transport mechanisms behind membrane adsorption, the advantages that membrane adsorbers possess as compared to column chromatography, the various membrane surface chemistries that can be created, and the application of membrane chromatography in the purification of a wide variety of proteins. In this review, the basic concept of membrane chromatography is explained, which includes a discussion of the factors that control membrane performance and how that performance can be optimized. The diverse ligand chemistries that have been used to create the three main families of membrane adsorbers that is affinity, hydrophobic interaction, and ion exchange, are described along with available membrane adsorber formats. Finally, applications of membrane adsorbers throughout a standard protein purification train, from the direct capture of proteins from unclarified feedstocks to the clearance of trace impurities in final polishing steps, will be demonstrated using small‐ and pilot‐scale examples.

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Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies

Cited by 203 publications

References 161 publications

Monoliths in Bioprocess Technology

Monoliths in Bioprocess Technology

Interconnected Porous Material Prepared Via High Internal Phase Emulsion Stabilized by Mixture of Fe₃O₄and Tween85

Membrane Chromatography

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Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies

Cited by 203 publications

References 161 publications

Monoliths in Bioprocess Technology

Monoliths in Bioprocess Technology

Interconnected Porous Material Prepared Via High Internal Phase Emulsion Stabilized by Mixture of Fe3O4and Tween85

Membrane Chromatography

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Product

Resources

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Interconnected Porous Material Prepared Via High Internal Phase Emulsion Stabilized by Mixture of Fe₃O₄and Tween85