Polymersome‐Loaded Capsules for Controlled Release of DNA

Abstract

The formation of a novel drug-delivery carrier for the controlled release of plasmid DNA that comprises layer-by-layer polymer capsules subcompartmentalized with pH-sensitive nanometer-sized polymersomes is reported. The amphiphilic diblock copolymer poly(oligoethylene glycol methacrylate)-block-poly(2-(diisopropylamino)ethyl methacrylate) forms polymersomes at physiological pH, but transitions to unimeric polymer chains upon acidification to cellular endocytic pH. These polymersomes can thus release an encapsulated payload in response to a change in pH from physiological to endocytic conditions. Multicomponent layer-by-layer capsules are formed by exploiting the ability of tannic acid to act as an efficient hydrogen-bond donor for both the polymersomes and poly(N-vinyl pyrrolidone) at physiological pH. These capsules show release of a plasmid DNA payload encapsulated within the polymersome subcompartments in response to changes in pH between physiological and endocytic conditions.

Biodegradable capsules as non-viral vectors for in vitro delivery of PEI/siRNA polyplexes for efficient gene silencing

“…to create a multifunctional delivery system [15][16][17], and they are steadily internalized by mammalian cells via phagocytosis and lipid-raft-mediated macropinocytosis [18,19]. Polymeric capsules have already been used for several sensing and drug delivery applications [20][21][22] including DNA delivery [23,24]. However, there are only few reports on siRNA delivery [25,26].…”

Nanotechnological Approaches for Genetic Immunization

“…Amphiphilic diblock copolymer of poly (oligoethylene glycol methacrylate)-block-poly(2-(diisopropylamino)ethyl methacrylate in association with tannic acid forms DNA-loaded polymersomes. Developed systems have demonstrated better cytosolic release of encapsulated nucleic acid materials (Lomas et al 2011). Further, calcein-loaded polymersomes have also observed for their cytosolic delivery within dendritic cell (Scott et al 2012).…”

Phenolic film engineering for template-mediated microcapsule preparation

“…Lomas et al 52 used TA to construct polymersome-loaded multicompartment capsules (Figure 7). Polymersomes formed from poly(oligo(ethylene glycol) methacrylate)-block-poly(2-(diisopropylamino)-ethyl methacrylate) were incorporated into multilayers of TA and poly(N-vinylpyrrolidone).…”