Polymerization of Ion-Pair Comonomers of Related Structures

Salamone, J. C.; Quach, Loc; Watterson, Arthur C.; Krauser, Scott F.; Mahmud, M. U.

doi:10.1080/00222338508056628

Cited by 41 publications

(35 citation statements)

References 11 publications

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“…2-Methacryloyloxyethyl tri-methyl ammonium chloride (METAC) was purified with charcoal before use, according to the literature. 24 …”

Section: Monomer Purificationmentioning

confidence: 97%

“…The monomer purity was confirmed by gas chromatography, using a Hewlett-Packard instrument, Model 5971 A, equipped with a HP-5 column and an UV detector. 2-Acrylamido-2-methylpropane-sulfonic acid (AMPS) was purified according to the literature 24 by recrystallizing the commercial monomer in anhydrous methanol. AMPS purity was evaluated with differential scanning calorimetry (melting point) using a Dupont Model 2910.…”

Section: Monomer Purificationmentioning

confidence: 99%

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Quantitative evaluation of bacteria adherent to polyelectrolyte HEMA‐based hydrogels

Berlutti

Rosso

Bosso

et al. 2003

J Biomedical Materials Res

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The use of adhesive poly(HEMA)-based hydrogels is standard practice in dental restorative procedures. Microorganisms, which potentially can cause oral pathologies, may colonize these polymers. In the present work, bacterial adhesion to polymers prepared with 2-hydroxyethyl methacrylate (HEMA) and to different molar ratios of 2-acrylamido-2-methylpropane-sulfonic acid (AMPS) and/or to 2-methacryloyloxyethyl-tri-methyl-ammonium chloride (METAC) co-monomers were tested. A colorimetric assay system that utilizes the Microbo revelation medium (Microbo srl, Rome, Italy) for microbial counts is shown to be capable of counting the number of adherent bacterial cells without removing them from polymer surfaces. In conditions that mimic those present in the oral cavity, similar bacterial adhesion percentages on the same polymer were observed with the different bacteria belonging to both gram-positive and gram-negative genera, such as Streptococcus sobrinus and Streptococcus oralis (resident microorganisms in the oral cavity) and Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa (transient microorganisms in the oral cavity). It is determined that the physico-chemical characteristics of poly(HEMA)-based hydrogels are the major factors promoting bacterial adhesion, which increased with increasing water content in the swollen polymers, reaching maximal values on the cationic polymers.

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“…2-Methacryloyloxyethyl tri-methyl ammonium chloride (METAC) was purified with charcoal before use, according to the literature. 24 …”

Section: Monomer Purificationmentioning

confidence: 97%

Section: Monomer Purificationmentioning

confidence: 99%

Quantitative evaluation of bacteria adherent to polyelectrolyte HEMA‐based hydrogels

Berlutti

Rosso

Bosso

et al. 2003

J Biomedical Materials Res

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“…The synthesis of pHEMA and pHEMA-co-METAC copolymers was performed according to the literature (Salamone et al, 1985).…”

Section: Polymer Synthesis and Characterizationmentioning

confidence: 99%

“…METAC was purified with activated charcoal, according to a previously described method (Salamone et al, 1985).…”

Section: Polymers Synthesis and Characterizationmentioning

confidence: 99%

Cationic polyelectrolyte hydrogel fosters fibroblast spreading, proliferation, and extracellular matrix production: Implications for tissue engineering

Rosa

Cartenı̀

Petillo

et al. 2003

Journal Cellular Physiology

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Fibrous encapsulation is known to occur to many prosthetic implants and is thought to be due to the cells not adhering adequately to the surface. For developing new materials able to enhance cellular adhesion by mimicking extracellular matrix components, polyelectrolyte polymers, characterized by tunable surface charges, have been proposed. Here we demonstrate that panoply of cell functions over a two-dimensional substratum is influenced by surface charge. We have at first generated structurally related polyelectrolyte substrata varying in their positive surface charge amount and subsequently evaluated a variety of behaviors of human primary fibroblasts seeded on these polymers. The proportion of adherent, spreading, and proliferating cells was increased significantly on cationic hydrophilic surfaces when compared with the neutral base surface. The extent of cell spreading correlated with cytoskeleton organization as assessed using immunofluorescence techniques. In the key experiment, the presence of cationic charges on cell adhesion-resistant neutral surface increased the synthesis of collagen I and III, the release of their metabolites, and the expression of their mRNA by fibroblasts. Interestingly, the scarce collagen deposits on neutral polymer consisted, for the most part, of collagen I while collagen III was present only in traces probably due to the secretion of metalloproteinase-2 by non-adherent fibroblasts. Taken together, these results show that polyelectrolyte films may promote the attachment of fibroblast cells as well as their normal secretory phenotype. Both effects could be potentially useful in integrating soft connective tissue to the implant, decreasing the chance of its fibrous encapsulation.

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“…[14,15] For this study, four different polymerizable ion pairs were synthesized from inexpensive precursors (Fig. 2) 2-acrylamido-2-methyl-1-propane sulfonate (APTA/AMPS), and a mixed methylmethacrylate-styrene pair, (4-vinylbenzyl)dimethylhexan-1-ammonium 2-(methacryloyloxy)ethane sulfonate [16] (VBDHA/MES).…”

mentioning

confidence: 99%