Polymerization of Actin from Maize Pollen

Yen, Lung-Fei; Xiong, Liu; Cai, Sanjun

doi:10.1104/pp.107.1.73

Cited by 12 publications

(6 citation statements)

References 17 publications

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“…The actin accumulates in substantial amounts in mature pollen grains and possesses properties that are closely similar to muscle actin (172). Thus, pollen-derived actin can activate myosin ATPase activity and bind muscle heavy meromyosin, yielding the characteristic arrow head decoration (172).…”

Section: Actin Microfilamentsmentioning

confidence: 99%

Pollen Germination and Tube Growth

Taylor

Hepler

1997

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

724

575

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Many aspects of Angiosperm pollen germination and tube growth are discussed including mechanisms of dehydration and rehydration, in vitro germination, pollen coat compounds, the dynamic involvement of cytoskeletal elements (actin, microtubules), calcium ion fluxes, extracellular matrix elements (stylar arabinogalactan proteins), and control mechanisms of gene expression in dehydrating and germinating pollen. We focus on the recent developments in pollen biology that help us understand how the male gamete survives and accomplishes its successful delivery to the ovule of the sperm to effect sexual reproduction.

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Section: Actin Microfilamentsmentioning

confidence: 99%

Pollen Germination and Tube Growth

Taylor

Hepler

1997

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

724

575

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“…Actin proteins show about 80±85% similarity in animals and plants. Liu and co-workers found maize pollen actin had similar characteristics to actin of animal non-muscle cells Yen 1992, 1995;Yen et al 1995). Andersland et al (1992) succeeded in purifying actin from pea roots by DNase I anity chromatography.…”

Section: Introductionmentioning

confidence: 94%

Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii

Zee

Liu

et al. 2001

Planta

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The distribution of and relationship between F-actin and G-actin were investigated in pollen grains and pollen tubes of Lilium davidii Duch. using a confocal laser scanning microscope after fluorescence and immunofluorescence labeling. Circular F-actin bundles were found to be the main form of microfilament cytoskeleton in pollen grains and pollen tubes. Consistent with cytoplasmic streaming in pollen tubes, there were no obvious F-actin bundles in the 10- to 20-microm tip region of long pollen tubes, only a few short F-actin fragments. Labeling with fluorescein isothiocyanate (FITC)-DNase I at first established the presence of a tip-focused gradient of intracellular G-actin concentration at the extreme apex of the tube, the concentration of G-actin being about twice as high in the 10- to 20-microm region of the tip as in other regions of the pollen tube. We also found that the distribution of G-actin was related negatively to that of the F-actin in pollen tubes of L. davidii. Caffeine treatment caused the G-actin tip-focused gradient to disappear, and F-actin to extend into the pollen tube tip. Based on these results, we speculate that the circular F-actin bundles may be the track for bidirectional cytoplasmic streaming in pollen tubes, and that in the pollen tube tip most of the F-actin is depolymerized into G-actin, leading to the absence of F-actin bundles in this region.

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“…Actin occurs in plant cells in the form of monomers (G-actin), oligomers, or short AFs composed of few monomers and their meshworks, long AFs, and their more complex assemblies, represented by thick bundles and dense networks of F-actin. Plant actin was isolated either in the form of F-actin after phalloidin treatment (Abe and Davies, 1991) or G-actin originating from diverse sources (Vahey and Scordilis, 1980;Ma and Yen, 1989;Liu and Yen, 1992;Koropp and Volkmann, 1994;Andersland et al, 1994;Yen et al, 1995;Ren et al, 1997). However, except for the pioneering results from Staiger's group (Ren et al, 1997), detailed biochemical and structural characterizations of plant actin in vitro, as they have been reported for actin from animal sources (e.g., Kabsch and Vandekerckhove 1992), are still lacking.…”

Section: Actin and Actin-associated Moleculesmentioning

confidence: 99%

Actin cytoskeleton in plants: From transport networks to signaling networks

Volkmann

Baluška

1999

Microsc. Res. Tech.

144

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The plant actin cytoskeleton is characterized by a high diversity in regard to gene families, isoforms, and degree of polymerization. In addition to the most abundant F‐actin assemblies like filaments and their bundles, G‐actin obviously assembles in the form of actin oligomers composed of a few actin molecules which can be extensively cross‐linked into complex dynamic meshworks. The role of the actomyosin complex as a force generating system — based on principles operating as in muscle cells — is clearly established for long‐range mass transport in large algal cells and specialized cell types of higher plants. Extended F‐actin networks, mainly composed of F‐actin bundles, are the structural basis for this cytoplasmic streaming of high velocities On the other hand, evidence is accumulating that delicate meshworks built of short F‐actin oligomers are critical for events occurring at the plasma membrane, e.g., actin interventions into activities of ion channels and hormone carriers, signaling pathways based on phospholipids, and exo‐ and endocytotic processes. These unique F‐actin arrays, constructed by polymerization‐depolymerization processes propelled via synergistic actions of actin‐binding proteins such as profilin and actin depolymerizing factor (ADF)/cofilin are supposed to be engaged in diverse aspects of plant morphogenesis. Finally, rapid rearrangements of F‐actin meshworks interconnecting endocellular membranes turn out to be especially important for perception‐signaling purposes of plant cells, e.g., in association with guard cell movements, mechano‐ and gravity‐sensing, plant host–pathogen interactions, and wound‐healing. Microsc. Res. Tech. 47:135–154, 1999. © 1999 Wiley‐Liss, Inc.

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Polymerization of Actin from Maize Pollen

Cited by 12 publications

References 17 publications

Pollen Germination and Tube Growth

Pollen Germination and Tube Growth

Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii

Actin cytoskeleton in plants: From transport networks to signaling networks

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