Polymerization and matrix physical properties as important design considerations for soluble collagen formulations

Kreger, S.T.; Bell, Brett; Bailey, Jennifer Lynne; Stites, E.; Kuske, J.; Waisner, Beverly; Voytik‐Harbin, Sherry L.

doi:10.1002/bip.21431

Cited by 125 publications

(260 citation statements)

References 70 publications

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“…This latter point may be critical given the tissue complexities and multiple interactions that we have reviewed, and optimizing the 3-D culture system to include ECM components reminiscent of the developing pancreas should be considered. One could also find ways of tricking hESC grown in vitro into responding as if they were in the normal 3-D environment, and approaching novel biopolymers for support and differentiation (Kreger et al, 2010), even in combination with nanoscale bioengineering (von der Mark et al, 2010), could be excellent supplemental directions.…”

Section: Es Cell Differentiation Systems and Small Molecule Library Smentioning

confidence: 99%

Pancreas organogenesis: From bud to plexus to gland

Pan

Wright

2011

Developmental Dynamics

524

594

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Pancreas oganogenesis comprises a coordinated and highly complex interplay of signaling events and transcriptional networks that guide a step-wise process of organ development from early bud specification all the way to the final mature organ state. Extensive research on pancreas development over the last few years, largely driven by a translational potential for pancreatic diseases (diabetes, pancreatic cancer, and so on), is markedly advancing our knowledge of these processes. It is a tenable goal that we will one day have a clear, complete picture of the transcriptional and signaling codes that control the entire organogenetic process, allowing us to apply this knowledge in a therapeutic context, by generating replacement cells in vitro, or perhaps one day to the whole organ in vivo. This review summarizes findings in the past 5 years that we feel are amongst the most significant in contributing to the deeper understanding of pancreas development. Rather than try to cover all aspects comprehensively, we have chosen to highlight interesting new concepts, and to discuss provocatively some of the more controversial findings or proposals. At the end of the review, we include a perspective section on how the whole pancreas differentiation process might be able to be unwound in a regulated fashion, or redirected, and suggest linkages to the possible reprogramming of other pancreatic cell-types in vivo, and to the optimization of the forward-directed-differentiation of human embryonic stem cells (hESC), or induced pluripotential cells (iPSC), towards mature b-cells. Developmental Dynamics 240:530-565,

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Section: Es Cell Differentiation Systems and Small Molecule Library Smentioning

confidence: 99%

Pancreas organogenesis: From bud to plexus to gland

Pan

Wright

2011

Developmental Dynamics

524

594

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“…12,20,37 Relationships between collagen source and polymerization kinetics, hydrogel mechanics, and fiber structure are discussed in more detail in ''Polymerization,'' ''Mechanics,'' and ''Structure'' sections. Further, the method by which collagen is extracted from tissue has been shown to alter the molecular structure of the collagen fibrils as well as the kinetics of assembly.…”

Section: Collagen Source and Solubilizationmentioning

confidence: 99%

“…Although collagen I is a viable scaffold for a wide range of applications, [12][13][14][15] comparison between studies is difficult due to significant variation in hydrogel fabrication protocols used by different research groups. It is well known that scaffold material properties play an important role in cellular behavior.…”

Section: Introductionmentioning

confidence: 99%

“…It is well known that scaffold material properties play an important role in cellular behavior. 10,12 One of the main drawbacks to using collagen hydrogels as scaffolds for tissue engineering is that these properties are highly variable and dependent on a large number of fabrication parameters, such as collagen source or gelation pH, resulting in a vast design space. 10,[16][17][18][19][20] The need for quantitative characterization of collagen hydrogels is recognized within the scientific community as a prerequisite for quantitative studies, tissue optimization, and comparative research.…”

Section: Introductionmentioning

confidence: 99%

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Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine

Vlachos

Rylander³

2014

Tissue Engineering Part B: Reviews

458

353

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Type I collagen hydrogels have been used successfully as three-dimensional substrates for cell culture and have shown promise as scaffolds for engineered tissues and tumors. A critical step in the development of collagen hydrogels as viable tissue mimics is quantitative characterization of hydrogel properties and their correlation with fabrication parameters, which enables hydrogels to be tuned to match specific tissues or fulfill engineering requirements. A significant body of work has been devoted to characterization of collagen I hydrogels; however, due to the breadth of materials and techniques used for characterization, published data are often disjoint and hence their utility to the community is reduced. This review aims to determine the parameter space covered by existing data and identify key gaps in the literature so that future characterization and use of collagen I hydrogels for research can be most efficiently conducted. This review is divided into three sections: (1) relevant fabrication parameters are introduced and several of the most popular methods of controlling and regulating them are described, (2) hydrogel properties most relevant for tissue engineering are presented and discussed along with their characterization techniques, (3) the state of collagen I hydrogel characterization is recapitulated and future directions are proposed. Ultimately, this review can serve as a resource for selection of fabrication parameters and material characterization methodologies in order to increase the usefulness of future collagenhydrogel-based characterization studies and tissue engineering experiments.

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“…NOTE: For this protocol, use liquid collagen (pH 1.9-2.2) from bovine hind (2.9-3.3 mg/ml collagen; 95% collagen type I, 5% collagen type IV). Examples of further collagen lattice preparation protocols using collagen type I from other sources, such as porcine or rat, are given in 32,33 . Do not change the volumes of the used solutions as this will alter the ultimate collagen concentration of 1.67 mg/ml of the lattice.…”

Section: Preparation Of Migration Chambersmentioning

confidence: 99%

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Rommerswinkel

Niggemann

Keil

et al. 2014

JoVE

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The ability to migrate is a hallmark of various cell types and plays a crucial role in several physiological processes, including embryonic development, wound healing, and immune responses. However, cell migration is also a key mechanism in cancer enabling these cancer cells to detach from the primary tumor to start metastatic spreading. Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell types. Because the locomotory behavior of cells markedly differs between a two-dimensional (2D) and three-dimensional (3D) environment it can be assumed that the analysis of the migration of cells that are embedded within a 3D environment would yield in more significant cell migration data. The advantage of the described 3D collagen matrix migration assay is that cells are embedded within a physiological 3D network of collagen fibers representing the major component of the extracellular matrix. Due to time-lapse video microscopy real cell migration is measured allowing the determination of several migration parameters as well as their alterations in response to pro-migratory factors or inhibitors. Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. Likewise, also cell clusters or spheroids could be embedded within the collagen matrix concomitant with analysis of the emigration of single cells from the cell cluster/ spheroid into the collagen lattice. We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a physiological-like 3D environment. Video LinkThe video component of this article can be found at

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Polymerization and matrix physical properties as important design considerations for soluble collagen formulations

Cited by 125 publications

References 70 publications

Pancreas organogenesis: From bud to plexus to gland

Pancreas organogenesis: From bud to plexus to gland

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

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