Polymeric Nanoparticles of Brazilian Red Propolis Extract: Preparation, Characterization, Antioxidant and Leishmanicidal Activity

Nascimento, Ticiano Gomes do; Silva, Priscilla Fonseca da; Azevedo, Lais Farias; Rocha, Louisianny Guerra da; Porto, Isabel Cristina Celerino de Moraes; Moura, Túlio Flávio Accioly de Lima e; Júnior, Irinaldo Diniz Basílio; Grillo, Luciano Aparecido Meireles; Dornelas, Camila Braga; Fonseca, E. J. S.; Oliveira, Elder Eldervitch Carneiro de; Zhang, Alex Tong; Watson, David

doi:10.1186/s11671-016-1517-3

Cited by 122 publications

(95 citation statements)

References 76 publications

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“…braziliensis in vitro [64]. All of the extracts herein confirmed the antioxidant effects of propolis, as evidenced by significant decreases in nitric oxide and superoxide production [65–69]. In addition, our results showed increased superoxide dismutase (SOD) activity following treatment by alcoholic and glycolic extracts.…”

Section: Discussionsupporting

confidence: 75%

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Rebouças-Silva

Celes

Lima

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Treatments based on antimonials to cutaneous leishmaniasis (CL) entail a range of toxic side effects. Propolis, a natural compound widely used in traditional medical applications, exhibits a range of biological effects, including activity against infectious agents. The aim of this study was to test the potential leishmanicidal effects of different propolis extracts against Leishmania (Viannia) braziliensis promastigotes and intracellular amastigotes in vitro. Stationary-phase L. (V) braziliensis promastigotes were incubated with medium alone or treated with dry, alcoholic, or glycolic propolis extract (10, 50, or 100 μg/mL) for 96 h. Our data showed that all extracts exhibited a dose-dependent effect on the viability of L. (V) braziliensis promastigotes, while controlling the parasite burden inside infected macrophages. Dry propolis extract significantly modified the inflammatory profile of murine macrophages by downmodulating TGF-β and IL-10 production, while upmodulating TNF-α. All three types of propolis extract were found to reduce nitric oxide and superoxide levels in activated L. braziliensis-infected macrophages. Altogether, our results showed that propolis extracts exhibited a leishmanicidal effect against both stages of L. (V) braziliensis. The low cell toxicity and efficient microbicidal effect of alcoholic or glycolic propolis extracts make them candidates to an additive treatment for cutaneous leishmaniasis.

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Section: Discussionsupporting

confidence: 75%

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Rebouças-Silva

Celes

Lima

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Specifically, we identified the plant‐based flavonoid drug genistein as having potential activity in determining the observed expression profiles in post‐treatment compared to pretreatment samples. This provides some support for the recent resurgence of interest in investigating the leishmanicidal activity of plant‐derived flavonoids. The observation that the quinoline alkaloid camptothecin was a potential upstream regulator was also of interest, especially given the progress of the orally active 8‐aminoquinoline sitamaquine (formerly WR‐6026) through preclinical and phase I/II clinical trials against VL in India, Brazil and Kenya (reviewed Ref.…”

Section: Discussionsupporting

confidence: 52%

Expression profiling of Sudanese visceral leishmaniasis patients pre‐ and post‐treatment with sodium stibogluconate

Salih

Fakiola

Lyons

et al. 2017

Parasite Immunology

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Summary Visceral leishmaniasis (VL) in Sudan caused by Leishmania donovani is fatal in susceptible individuals if untreated. Treatment with sodium stibogluconate (SSG) leads to post‐kala‐azar dermal leishmaniasis (PKDL) in 58% of patients. Here, Affymetrix microarrays were used to identify genes differentially expressed in lymph nodes (N=9 paired samples) pre‐ and post‐treatment with SSG. Using the Bioconductor package limma, 438 genes from 28 869 post‐quality‐control probe sets were differentially expressed (Pnominal≤.02) post‐ vs pretreatment. Canonical pathway analysis using Ingenuity Pathway Analysis™ identified “role of nuclear factor of activated T‐cell in regulation of immune response” (Pnominal=1.35×10−5; PBH‐adjusted=4.79×10−3), “B‐cell development” (Pnominal=2.04×10−4; PBH‐adjusted=.024), “Fcγ receptor‐mediated phagocytosis in macrophages and monocytes” (Pnominal=2.04×10−4; PBH‐adjusted=.024) and “OX40 signalling” (Pnominal=2.82×10−4; PBH‐adjusted=.025) as pathways differentially regulated post‐ vs pretreatment. Major network hub genes included TP53, FN1, MYC, BCL2, JUN, SYK, RUNX2, MMP1 and ACTA2. Top endogenous upstream regulators included IL‐7 (P=2.28×10−6), TNF (P=4.26×10−6), Amyloid Precursor Protein (P=4.23×10−5) and SPI1/PI.1 (P=1.17×10−7). Top predicted chemical drug regulators included the flavonoid genistein (P=4.56×10−7) and the quinoline alkaloid camptothecin (P=5.14×10−5). These results contribute to our understanding of immunopathology associated with VL and response to SSG treatment. Further replication could identify novel therapeutic strategies that improve on SSG treatment and reduce the likelihood of progression to PKDL.

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“…The addition of drugs was not a significant parameter to the degradation of the samples ( P > 0.05). Neomycin sulphate's thermal degradation occurs in temperatures above 94.9°C and for propolis occurs above 81.7°C, both relative to the dehydration of the material. It was not possible to observe the influence of these components in the samples' degradation, probably due to their low concentration in the polymeric matrix.…”

Section: Resultsmentioning

confidence: 98%

Alginate hydrogels incorporating neomycin or propolis as potential dressings for diabetic ulcers: Structure, swelling, and antimicrobial barrier properties

Candido

Conceição

Moreira

et al. 2019

Polymers for Advanced Techs

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Alginate hydrogels have many attractive characteristics for potential use as wound dressing materials. However, they are not considered to possess any intrinsic activity against microbial infection, often present in neuropathic wounds. To overcome this, the effect of incorporating neomycin or propolis in alginate hydrogels was investigated, both by direct blending alone and also by further addition of loaded alginate microparticles prepared by the extrusion dripping method. The morphological, microstructural, thermal, mechanical, and swelling properties of each film were evaluated, as well as particle size distribution and antimicrobial penetration analysis. Microparticle size was considered suitable for drug delivery applications and incorporation in hydrogel films. The presence of neomycin and propolis, in both blended and microparticle form, interfered with film properties leading to hydrogels with different characteristics. All samples showed swelling degrees up to 100% and mechanical and thermal properties suitable for application as wound dressings. In addition, all samples acted as barriers to microbial penetration.

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Polymeric Nanoparticles of Brazilian Red Propolis Extract: Preparation, Characterization, Antioxidant and Leishmanicidal Activity

Cited by 122 publications

References 76 publications

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Expression profiling of Sudanese visceral leishmaniasis patients pre‐ and post‐treatment with sodium stibogluconate

Alginate hydrogels incorporating neomycin or propolis as potential dressings for diabetic ulcers: Structure, swelling, and antimicrobial barrier properties

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