Polymeric multilayers that localize the release of chlorhexidine from biologic wound dressings

Agarwal, Ankit; Nelson, Tyler B.; Kierski, Patricia R.; Schurr, Michael J.; Murphy, Christopher J.; Czuprynski, Charles J.; McAnulty, Jonathan F.; Abbott, Nicholas L.

doi:10.1016/j.biomaterials.2012.05.068

Cited by 78 publications

(65 citation statements)

References 52 publications

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“…Previous effort was based on layer-by-layer assembly [35, 36] and core-shell electrospinning [37] to achieve two drugs releasing in different manners. However, the diffusion between layers still remains a critical problem [38].…”

Section: Resultsmentioning

confidence: 99%

Dual growth factor releasing multi-functional nanofibers for wound healing

et al. 2013

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Section: Resultsmentioning

confidence: 99%

Dual growth factor releasing multi-functional nanofibers for wound healing

et al. 2013

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“…The SA culture was allowed to proliferate in suspension on a shaker at 170 rpm at 37 °C until desired growth phase optical density was achieved (OD 600 = 0.1, 1 × 10 4 CFU/mL) as measured by the spectrophotometer (Nanodrop 2000x UV–vis Spectrophotometer, Thermo Scientific). SA at OD 600 = 0.1 represents the pre-log growth phase (lag phase) in which bacteria will have to replicate at the wound site to establish a clinical infection, representing a wound infection model for most clinical cases [30,31]. …”

Section: Methodsmentioning

confidence: 99%

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

et al. 2017

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Mesenchymal stromal/stem cells (MSCs) have demonstrated pro-healing properties including an anti-inflammatory cytokine profile and the promotion of angiogenesis via expression of growth factors in pre-clinical models. MSCs encapsulated in poly(ethylene glycol) diacrylate (PEGdA) and thiolated gelatin poly(ethylene glycol) (Gel-PEG-Cys) crosslinked hydrogels have led to controlled cellular presentation at wound sites with favorable wound healing outcomes. However, the therapeutic potential of MSC-loaded hydrogels may be limited by non-specific protein adsorption on the delivery matrix that could facilitate the initial adhesion of microorganisms and subsequent virulent biofilm formation. Antimicrobials loaded concurrently in the hydrogels with MSCs could reduce microbial bioburden and promote healing, but the antimicrobial effect on the MSC wound healing capacity and the antibacterial efficacy of the hydrogels is unknown. We demonstrate that minocycline specifically induces a favorable change in MSC migration capacity, proliferation, gene expression, extracellular matrix (ECM) attachment, and adhesion molecule and growth factor release with subsequent increased angiogenesis. We then demonstrate that hydrogels loaded with MSCs, minocycline, vancomycin, and linezolid can significantly decrease bacterial bioburden. Our study suggests that minocycline can serve as a dual mechanism for the regenerative capacity of MSCs and the reduction of bioburden in triple antimicrobial-loaded hydrogels.

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“…7 Surface modications are commonly performed in three ways. 10,11 In the second approach, surfaces are designed to eradicate bacteria before colonization by releasing antibacterial species or antiseptic materials, such as chlorhexidine, 12 triclosan, 13 metal ions, 14 or combinations of these materials. For example, polyethylene glycol (PEG) and its derivatives can be used to prevent the adsorption of non-specically accumulated serum proteins that promote bacterial adherence and colonization, 8 or active biomolecules such as the cell-adhering peptide Arg-Gly-Asp (RGD) can be coupled to the surface to promote the adhesion of host cells to the device surface, thus preventing bacterial growth.…”

Section: Introductionmentioning

confidence: 99%

Compatibility balanced antibacterial modification based on vapor-deposited parylene coatings for biomaterials

et al. 2014

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Advanced antibacterial surfaces are designed based on covalently attached antibacterial agents, avoiding potential side effects associated with overdosed or eluted agents. The technique is widely applicable regardless of the underlying substrate material. In addition, antibacterial surfaces are effective against the early stages of bacterial adhesion and can significantly reduce the formation of biofilm, without compromising biocompatibility. Here, this concept was realized by employing a benzoyl-functionalized parylene coating. The antibacterial agent chlorhexidine was used as a proof of concept. Chlorhexidine was immobilized by reaction with photoactivated benzoyl-functionalized surfaces, including titanium alloy, stainless steel, polyether ether ketone, polymethyl methacrylate, and polystyrene. A low concentration of chlorhexidine (1.4 AE 0.08 nmol cm À2 ) covalently bound to surfaces rendered them sufficiently resistant to an Enterobacter cloacae inoculum and its adherent biofilm. Compared to unmodified surfaces, up to a 30-fold reduction in bacterial attachment was achieved with this coating technology. The immobilization of chlorhexidine was verified with infrared reflection absorption spectroscopy (IRRAS) and X-ray photoelectron spectroscopy (XPS), and a leaching test was performed to confirm that the chlorhexidine molecules were not dislodged. Cell compatibility was examined by culturing fibroblasts and osteoblasts on the modified surfaces, revealing greater than 93% cell viability.This coating technology may be broadly applicable for a wide range of other antibacterial agents and allow the design of new biomaterials.

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Polymeric multilayers that localize the release of chlorhexidine from biologic wound dressings

Cited by 78 publications

References 52 publications

Dual growth factor releasing multi-functional nanofibers for wound healing

Dual growth factor releasing multi-functional nanofibers for wound healing

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

Compatibility balanced antibacterial modification based on vapor-deposited parylene coatings for biomaterials

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