Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells

Characterization of particles fabricated with poly(D,L-lactic-co-glycolic acid) and an ornithine–histidine peptide as carriers of oligodeoxynucleotide for delivery into primary dendritic cells

“…2). In MS O10H6 , the DNA is adsorbed on the microsphere surface via ionic interactions and can be dislodged by heparin, an anionic polysaccharide [19,20]. In contrast, the DNA is embedded in PLGA O10H6 -ODN and cannot be released by surface displacement with heparin (data not shown).…”

“…Our laboratory has developed two forms of ODN carriers: the PLGA O10H6 described here and the MS O10H6 reported in a previous paper [19]. The particles have opposite zeta potentials with MS O10H6 -ODN carrying a positive charge [19] and PLGA O10H6 -ODN carrying a negative charge (Fig.…”

“…The particles have opposite zeta potentials with MS O10H6 -ODN carrying a positive charge [19] and PLGA O10H6 -ODN carrying a negative charge (Fig. 2).…”

“…We have previously shown that O10H6 forms stable condensates with nucleic acids on the surface of carboxylate polystyrene microspheres and this self-assembled system can facilitate DNA uptake in DCs [19,20]. The imidazole in histidine facilitates endosomal escape by disrupting the osmotic balance of the vesicles [21,22].…”

Encapsulation of NF-κB decoy oligonucleotides within echogenic liposomes and ultrasound-triggered release

“…Of particular concern are their short half-life [5, 6] and limited cellular or tissue uptake [7]. A number of vectors have been developed to prevent ODN from degradation by encapsulating ODN with some form of protective coating, including liposomes [8], hemagglutinating virus of Japan (HVJ)-liposomes [9], polymeric microspheres [10], and a variety of nanoparticles [11]. Also, several methodologies have been developed to enhance cellular uptake of the decoys, including pressure-mediated transfer [12], electrically enhanced transfer, and biolistic bombardment [13].…”