Polymeric entrapped thiol-coated gold nanorods: cytotoxicity and suitability as molecular optoacoustic contrast agent

Franchini, Mauro Comes; Ponti, Jessica; Lemor, Robert; Fournelle, Marc; Broggi, Francesca; Locatelli, Erica

doi:10.1039/c0jm02209h

Cited by 20 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells survived until a GNR concentration of 2 nM as it corresponds with several literature data. [14,[16][17][18] After PEG-GNR injection, Hematoxylin and Eosin Stain of mouse liver following did not show any pathological effects during month ( fig. 5) and the data is similar with publications.…”

Section: Resultsmentioning

confidence: 92%

Optoacoustic tomography in preclinical research: in vivo distribution of highly purified PEG-coated gold nanorods

Liopo

Ermilov

et al. 2011

SPIE Proceedings

View full text Add to dashboard Cite

We report on the optoacoustic (OA) imaging of the whole mouse body using a biocompatible contrast agent -highly purified, pegylated gold nanorods (GNR) -which has strong optical absorption in the near-infrared region and low level of toxicity. In vitro toxicity studies showed no significant change in survival rates of the cultured normal epithelium IEC-6 cells when incubated for 24 hours with up to 1 nM of GNR. In vivo toxicity studies in nude mice showed no pathological changes in liver 1 month after the IV injection of GNR with intra-body concentration around 0.25-0.50 nM. In order to study the enhancement of the OA contrast and accumulation of GNR in different tissues, we performed 3D OA imaging of live nude mice with IV-injected GNR. The enhancement of the OA contrast in comparison with the images of the untreated mice was visible starting 1 hour after the GNR injection. The OA contrast of kidneys, liver, and spleen peaked at about 2-3 days after the administration of the GNR, and then was gradually reducing.

show abstract

Section: Resultsmentioning

confidence: 92%

Optoacoustic tomography in preclinical research: in vivo distribution of highly purified PEG-coated gold nanorods

Liopo

Ermilov

et al. 2011

SPIE Proceedings

View full text Add to dashboard Cite

show abstract

“…The new disulfides 1-2 proved to be very efficient for the coating of the two nanostructured surfaces of GNRs and of AgNPs ( Figure 1). Indeed, GNRs-CTAB and its ligand exchange with disulfide 1 to give lipophilic GNRs-1 was achieved using our reported methods [13] and this procedure was also used for the coating of commercially available Ag NPs with disulfide 2 giving AgNPs-2 (see Figures S1-S4 in the Supporting Information). On the other hand, disulfide 3 has been also already reported by us for the coating of GNRs [13] As reported by OBrien, [6] the concept of high dilution of solvent appears to be necessary for the covalent linkage of the reactive groups that are in the outer shell of NPs but, in our case, we decided to dilute the functional group onto the surface making a coating with a mixture of ligands.…”

mentioning

confidence: 99%

“…[10] One of the most popular reactions within the click chemistry concept is the azide/alkyne Huisgen cycloaddition. [11] We earlier have reported [12,13] of lipophilic GNRs entrapped into PEG-based polymeric NPs and their suitability as optoacoustic contrast agents. [12] Herein, we have investigated how the chemical assembly of GNRs with silver nanoparticles influences the optical properties.…”

mentioning

confidence: 99%

“…Conventional GNRs-PNPs, as already reported by us [13] without Ag-NPs, were used for as reference. Optoacoustic [24] signal amplitudes of phantoms containing either GNRsPNPs or click-PNPs at the same concentration were investi- www.chemeurj.org gated using a Nd:YAG laser at its fundamental wavelength of 1064 nm and an optoacoustic imaging platform (DiPhAS, Fraunhofer IBMT).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Click Chemistry for the Assembly of Gold Nanorods and Silver Nanoparticles

Locatelli

Ori

Fournelle

et al. 2011

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

The synthesis of compact nanostructures with highly integratedfunctionalities through the controlled assembly ofnanoparticles (NPs) is potentially of broad interest in researchfields such as drug delivery, multimodal imaging, andelectronic devices. This concept seems to be particularlyimportant in view of the emerging concept of theranostic,according to which both therapeutic and diagnostic capabilitiescan be present in two nanostructures. A key step, however,is how to combine individual nanostructures withoutloosing the original properties

show abstract

“…22 The obtained GNRs-1@PMs were characterized by means of dynamic light scattering, atomic absorption analysis, transmission electron microscopy, UV-vis analysis, and gravimetric analysis. The dynamic light scattering showed a hydrodynamic diameter of 119.0±1.6 nm with a narrow size distribution (Polidispersity index =0.282±0.030) and a ζ-potential value of -41.6 mV (Figure 2A).…”

Section: Analysis Of Gnrs-1/curc@pmsmentioning

confidence: 99%

Controlled release of curcumin from curcumin-loaded nanomicelles to prevent peritendinous adhesion during Achilles tendon healing in rats

Zhang¹,

Li²,

Franchini³

et al. 2016

IJN

Self Cite

View full text Add to dashboard Cite

We introduced curcumin-loaded nanomicelles into a tendon-healing model to evaluate their effects on tendon healing and adhesion. Three groups consisting of 36 rats underwent rupture and repair of the Achilles tendon. The treatment group received an injection of curcumin-loaded nanomicelles (gold nanorods [GNRs]-1/curcumin in polymeric nanomicelles [curc@PMs] at a dosage of 0.44 mg curcumin/kg in 0.1 mL saline) into the surgical site and exposed to laser postoperatively at weeks 1, 2, and 3, for three times 10 seconds each, on the surgical site in the rats that underwent tendon rupture and repair, while the other two groups received 0.44 mg curcumin/kg in 0.1 mL saline and 0.1 mL of saline, respectively. The specimens were harvested at 4 weeks and subjected to biomechanical and histological evaluation. The scoring results of tendon adhesion indicated that GNRs-1/curc@PMs group was in the lowest grade of peritendinous adhesions compared to the other groups. Histological assessment further confirmed the preventive effect of GNRs-1/curc@PMs on tendon adhesion. These findings indicated greater tendon strength with less adhesion in the group treated with GNRs-1/curc@PMs combined with laser exposure, and that nanoparticle-based therapy may be applied to prevent adhesion in clinical patients.

show abstract

Polymeric entrapped thiol-coated gold nanorods: cytotoxicity and suitability as molecular optoacoustic contrast agent

Cited by 20 publications

References 29 publications

Optoacoustic tomography in preclinical research: in vivo distribution of highly purified PEG-coated gold nanorods

Optoacoustic tomography in preclinical research: in vivo distribution of highly purified PEG-coated gold nanorods

Click Chemistry for the Assembly of Gold Nanorods and Silver Nanoparticles

Controlled release of curcumin from curcumin-loaded nanomicelles to prevent peritendinous adhesion during Achilles tendon healing in rats

Contact Info

Product

Resources

About