Polymeric Bioinks for 3D Hepatic Printing

Sarkar, Joyita; Kamble, Swapnil C.; Kashikar, Nilambari C.

doi:10.3390/chemistry3010014

Cited by 14 publications

(22 citation statements)

References 96 publications

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“…However, such low-viscosity bioinks affect the stability of the printed structures and the formation of new tissues ( Gu et al, 2020 ; Ma et al, 2020 ). Moreover, inkjet-based bioprinting is associated with problems that can cause the formation of nonuniform droplets, nozzle clogging, and physical stimuli on cells (such as thermal or mechanical stresses) ( Gu et al, 2020 ; Sarkar et al, 2021 ).…”

Section: Overview Of 3d Bioprintingmentioning

confidence: 99%

“…Unlike inkjet-based printing systems, in extrusion-based printing systems, bioinks in the form of a filament are subjected to a layer-by-layer process to produce 3D structures ( Jang, 2017 ; Sarkar et al, 2021 ). A continuous filament is extruded from a nozzle by using the force generated by pneumatic pressure or mechanical tools (piston and screw) ( Figure 1A ) ( Jang et al, 2016b ).…”

Section: Overview Of 3d Bioprintingmentioning

confidence: 99%

“…Alginate hydrogel is one of the most extensively used biomaterials in tissue engineering, such as for the heart, bone, cartilage, and liver ( Jang et al, 2018 ; Ye et al, 2019 ; Sarkar et al, 2021 ). Alginate is a biopolymer found in brown seaweed or algae ( Duin et al, 2019 ; Ye et al, 2019 ; Piras and Smith, 2020 ; Sarkar et al, 2021 ).…”

Section: Multicomponent Hydrogel Bioinks For 3d Bioprinting Of Liver ...mentioning

confidence: 99%

( Lee et al, 2000 ; Kong et al, 2002 ; Jang et al, 2018 ; Duin et al, 2019 ; Ye et al, 2019 ; Piras and Smith, 2020 ; Wu et al, 2020 ; Sarkar et al, 2021 ).…”

Section: Multicomponent Hydrogel Bioinks For 3d Bioprinting Of Liver ...mentioning

confidence: 99%

“…Alginate is a biopolymer found in brown seaweed or algae ( Duin et al, 2019 ; Ye et al, 2019 ; Piras and Smith, 2020 ; Sarkar et al, 2021 ). As alginate is a negatively charged polysaccharide, alginate hydrogel can be readily polymerized by reacting with multivalent cations, such as

( Lee et al, 2000 ; Kong et al, 2002 ; Jang et al, 2018 ; Duin et al, 2019 ; Ye et al, 2019 ; Piras and Smith, 2020 ; Wu et al, 2020 ; Sarkar et al, 2021 ). Moreover, alginate is biocompatible and controllable in terms of its mechanical properties and printability, thus making it applicable in several bioprinting techniques ( Suntornnond et al, 2017 ; Tai et al, 2019 ; Davoodi et al, 2020 ; Piras and Smith, 2020 ) and for fabricating various forms of tissue constructs ( Huang et al, 2012 ; Onoe et al, 2013 ; Andersen et al, 2015 ; Piras and Smith, 2020 ).…”

Section: Multicomponent Hydrogel Bioinks For 3d Bioprinting Of Liver ...mentioning

confidence: 99%

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Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

Kim

Lee

et al. 2022

Front. Bioeng. Biotechnol.

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Three-dimensional (3D)-printed in vitro tissue models have been used in various biomedical fields owing to numerous advantages such as enhancements in cell response and functionality. In liver tissue engineering, several studies have been reported using 3D-printed liver tissue models with improved cellular responses and functions in drug screening, liver disease, and liver regenerative medicine. However, the application of conventional single-component bioinks for the printing of 3D in vitro liver constructs remains problematic because of the complex structural and physiological characteristics of the liver. The use of multicomponent bioinks has become an attractive strategy for bioprinting 3D functional in vitro liver tissue models because of the various advantages of multicomponent bioinks, such as improved mechanical properties of the printed tissue construct and cell functionality. Therefore, it is essential to review various 3D bioprinting techniques and multicomponent hydrogel bioinks proposed for liver tissue engineering to suggest future directions for liver tissue engineering. Accordingly, we herein review multicomponent bioinks for 3D-bioprinted liver tissues. We first describe the fabrication methods capable of printing multicomponent bioinks and introduce considerations for bioprinting. We subsequently categorize and evaluate the materials typically utilized for multicomponent bioinks based on their characteristics. In addition, we also review recent studies for the application of multicomponent bioinks to fabricate in vitro liver tissue models. Finally, we discuss the limitations of current studies and emphasize aspects that must be resolved to enhance the future applicability of such bioinks.

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Section: Overview Of 3d Bioprintingmentioning

confidence: 99%

Section: Overview Of 3d Bioprintingmentioning

confidence: 99%

Section: Multicomponent Hydrogel Bioinks For 3d Bioprinting Of Liver ...mentioning

confidence: 99%

( Lee et al, 2000 ; Kong et al, 2002 ; Jang et al, 2018 ; Duin et al, 2019 ; Ye et al, 2019 ; Piras and Smith, 2020 ; Wu et al, 2020 ; Sarkar et al, 2021 ).…”

Section: Multicomponent Hydrogel Bioinks For 3d Bioprinting Of Liver ...mentioning

confidence: 99%

Section: Multicomponent Hydrogel Bioinks For 3d Bioprinting Of Liver ...mentioning

confidence: 99%

See 3 more Smart Citations

Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

Kim

Lee

et al. 2022

Front. Bioeng. Biotechnol.

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Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Asim

Tabish

Liaqat

et al. 2023

Adv Healthcare Materials

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Gelatin is a widely utilized bioprinting biomaterial due to its cell‐adhesive and enzymatically cleavable properties, which improve cell adhesion and growth. Gelatin is often covalently cross‐linked to stabilize bioprinted structures, yet the covalently cross‐linked matrix is unable to recapitulate the dynamic microenvironment of the natural extracellular matrix (ECM), thereby limiting the functions of bioprinted cells. To some extent, a double network bioink can provide a more ECM‐mimetic, bioprinted niche for cell growth. More recently, gelatin matrices are being designed using reversible cross‐linking methods that can emulate the dynamic mechanical properties of the ECM. This review analyzes the progress in developing gelatin bioink formulations for 3D cell culture, and critically analyzes the bioprinting and cross‐linking techniques, with a focus on strategies to optimize the functions of bioprinted cells. This review discusses new cross‐linking chemistries that recapitulate the viscoelastic, stress‐relaxing microenvironment of the ECM, and enable advanced cell functions, yet are less explored in engineering the gelatin bioink. Finally, this work presents the perspective on the areas of future research and argues that the next generation of gelatin bioinks should be designed by considering cell–matrix interactions, and bioprinted constructs should be validated against currently established 3D cell culture standards to achieve improved therapeutic outcomes.

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Bioinspired Bioinks for the Fabrication of Chemomechanically Relevant Standalone Disease Models of Hepatic Steatosis

Guagliano,

Volpini,

Sardelli

et al. 2024

Adv Healthcare Materials

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Hepatotoxicity‐related issues are poorly predicted during preclinical trials, as their relevance is limited by the inadequacy to screen all the non‐physiological subclasses of the population. These pitfalls could be solved by implementing complex in vitro models of hepatic physiology and pathologies in the preclinical phase. To produce these platforms, we focused on extrusion‐based bioprinting, since it allows to manufacture tridimensional cell‐laden constructs with controlled geometries, in a high‐throughput manner. We propose different bioinks, whose formulation was tailored to mimic the chemomechanical environment of hepatic steatosis, the most prevalent hepatic disorder worldwide. Internally crosslinked alginate hydrogels were chosen as structural components of the inks. Their viscoelastic properties (G’ = 512‐730 Pa and G’’ = 94‐276 Pa, depending on frequency) were tuned to mimic those of steatotic liver tissue. Porcine hepatic ECM was introduced as a relevant biochemical cue. Sodium oleate was added to recall the accumulation of lipids in the tissue. Downstream analyses on 14‐layered bioprinted structures cultured for 10 days revealed the establishment of steatotic‐like features (intracellular lipid vesicles, viability decrease up to ≈50%) without needing external conditionings. The presented bioinks are thus suitable to fabricate complex models of hepatic steatosis to be implemented in a high‐throughput experimental frame.This article is protected by copyright. All rights reserved

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Polymeric Bioinks for 3D Hepatic Printing

Cited by 14 publications

References 96 publications

Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

Review on Multicomponent Hydrogel Bioinks Based on Natural Biomaterials for Bioprinting 3D Liver Tissues

Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Bioinspired Bioinks for the Fabrication of Chemomechanically Relevant Standalone Disease Models of Hepatic Steatosis

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