Polymeric 3D scaffolds for tissue regeneration: Evaluation of biopolymer nanocomposite reinforced with cellulose nanofibrils

Campodoni, Elisabetta; Heggset, Ellinor B.; Rashad, Ahmad; Ramírez-Rodríguez, Gloria Belén; Mustafa, Kamal; Syverud, Kristin; Tampieri, Anna; Sandri, Monica

doi:10.1016/j.msec.2018.10.026

Cited by 41 publications

(47 citation statements)

References 55 publications

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“…However, the degradation of collagen is faster than tissue regeneration, so that a cross-linking agent (BDDGE) was used to increase and modulate the stability of the scaffold in a physiological environment to an extent that is sufficient to allow cells engraftment and the deposition of new cellular matrix [ 38 ]. In the same conditions of alveolar bone, the desired structures with a high and interconnected porosity was obtained through a freeze-drying technique [ 13 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…The cross-linking process is a suitable tool to increase the chemical stability of bio-polymeric and biomineralized hybrids, and their resistance to enzymatic attack, as well as to tailor fiber organization, pore size, and porosity extent. On the basis of previous studies, we selected genipin as a cross-linking agent for hybrid Coll/FeHA, and BDDGE as a cross-linking agent for the pure bio-polymeric Coll construct mimicking the PDL [ 13 , 35 , 39 ]. The ability to adjust morphological features is promising to modulate the mechanical properties and stiffness of the hybrids, and to obtain scaffolds with biomechanical features acting as instructors for the cells [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Electrospinning is a versatile technology used to produce highly porous and thin nonwoven mats of nanometric fibrils, also by using bio-erodible polymers embedding inorganic particles [ 11 , 12 ]. Freeze-drying of hydrogels is a powerful technique that can be directed to obtain 3D scaffolds with highly interconnected porous morphology, by controlling the sublimation kinetics of the solvents [ 13 , 14 ]. In attempting to generate hybrid, mineralized gels, in a previous study, a bio-inspired process was applied to obtain the heterogeneous nucleation of nanocrystalline ion-doped apatite onto self-assembling Type I collagen fibrils (Coll) [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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A Graded Multifunctional Hybrid Scaffold with Superparamagnetic Ability for Periodontal Regeneration

Sprio

Campodoni

Sandri

et al. 2018

IJMS

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The regeneration of dental tissues is a still an unmet clinical need; in fact, no therapies have been completely successful in regenerating dental tissue complexes such as periodontium, which is also due to the lack of scaffolds that are able to guide and direct cell fate towards the reconstruction of different mineralized and non-mineralized dental tissues. In this respect, the present work develops a novel multifunctional hybrid scaffold recapitulating the different features of alveolar bone, periodontal ligament, and cementum by integrating the biomineralization process, and tape casting and electrospinning techniques. The scaffold is endowed with a superparamagnetic ability, thanks to the use of a biocompatible, bioactive superparamagnetic apatite phase, as a mineral component that is able to promote osteogenesis and to be activated by remote magnetic signals. The periodontal scaffold was obtained by engineering three different layers, recapitulating the relevant compositional and microstructural features of the target tissues, into a monolithic multifunctional graded device. Physico-chemical, morphological, and ultrastructural analyses, in association with preliminary in vitro investigations carried out with mesenchymal stem cells, confirm that the final scaffold exhibits a good mimicry of the periodontal tissue complex, with excellent cytocompatibility and cell viability, making it very promising for regenerative applications in dentistry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Graded Multifunctional Hybrid Scaffold with Superparamagnetic Ability for Periodontal Regeneration

Sprio

Campodoni

Sandri

et al. 2018

IJMS

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“…where W s was the weight of the swollen sample at a specific time point and W d was the initial weight of the dried sample (Campodoni et al, 2019).…”

Section: ×100mentioning

confidence: 99%

“…The creep tests were carried out at different stress in the range of 0.001-0.1 MPa to study the linearity zone and choose the same stress (0.01 MPa) to use in the following creep test. The samples, after an isothermal period at 37 • C of 5 min, were subjected at the defined stress for 15 min and afterwards they were left without any stress for 15 min (recovery time) (n = 3) (Campodoni et al, 2019).…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Mimicking Natural Microenvironments: Design of 3D-Aligned Hybrid Scaffold for Dentin Regeneration

Campodoni

Dozio

Panseri

et al. 2020

Front. Bioeng. Biotechnol.

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Tooth loss is a common consequence of a huge number of causes and can decrease the quality of humans' life. Tooth is a complex organ composed of soft connective tissues and mineralized tissues of which dentin is the most voluminous component whose formation is regulated by a very complex process displaying several similarities with osteogenesis. Calcium phosphates, in particular hydroxyapatite (HA), is the phase present in higher amount into the structure of dentin, characterized by microscopic longitudinal dentinal tubules. To address the challenge of dental tissue regeneration, here we propose a novel biomimetic approach, to design hybrid scaffolds resembling the physico-chemical features of the natural mineralized tissues, suitable to recreate an appropriate microenvironment that stimulates cell colonization and proliferation, therefore effective for improving regenerative approach in dental applications. Biomineralization is the adopted synthesis as a nature inspired process consisting in the nucleation of magnesium-doped-hydroxyapatite (MgHA) nanocrystals on the gelatin (Gel) matrix generating hybrid flakes (Gel/MgHA) featured by a Gel:MgHA weight ratio close to 20:80 and size of 50-70 µm. Chemical and topotactic constrains affect the formation of MgHA mineral phase on the organic template, generating quasiamorphous MgHA as revealed by XRD analysis and Ca/P ratio lower than 1.67, resembling the chemical and biological features of the natural apatite. The Gel/MgHA was then merged into the polymeric blend made of chitosan (Chit) and Gel to obtain a 3D porous scaffold with polymers: MgHA weight ratio of 40:60 and featured by an aligned porous structure as obtained by controlled freeze-drying process. The overall composite shows a swelling ratio of about 15 times after 6 h in PBS. The chemical stability was assured by means of a dehydrothermal cross-linking treatment (DHT) keeping the degradation lower than 20% after 28 days, while cell adhesion and proliferation were evaluated using a mouse fibroblast cell line.

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3D Bioprinting of Lignocellulosic Biomaterials

Shavandi

Hosseini

Okoro

et al. 2020

Adv Healthcare Materials

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The interest in bioprinting of sustainable biomaterials is rapidly growing, and lignocellulosic biomaterials have a unique role in this development. Lignocellulosic materials are biocompatible and possess tunable mechanical properties, and therefore promising for use in the field of 3D‐printed biomaterials. This review aims to spotlight the recent progress on the application of different lignocellulosic materials (cellulose, hemicellulose, and lignin) from various sources (wood, bacteria, and fungi) in different forms (including nanocrystals and nanofibers in 3D bioprinting). Their crystallinity, leading to water insolubility and the presence of suspended nanostructures, makes these polymers stand out among hydrogel‐forming biomaterials. These unique structures give rise to favorable properties such as high ink viscosity and strength and toughness of the final hydrogel, even when used at low concentrations. In this review, the application of lignocellulosic polymers with other components in inks is reported for 3D bioprinting and identified supercritical CO2 as a potential sterilization method for 3D‐printed cellulosic materials. This review also focuses on the areas of potential development by highlighting the opportunities and unmet challenges such as the need for standardization of the production, biocompatibility, and biodegradability of the cellulosic materials that underscore the direction of future research into the 3D biofabrication of cellulose‐based biomaterials.

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Polymeric 3D scaffolds for tissue regeneration: Evaluation of biopolymer nanocomposite reinforced with cellulose nanofibrils

Cited by 41 publications

References 55 publications

A Graded Multifunctional Hybrid Scaffold with Superparamagnetic Ability for Periodontal Regeneration

A Graded Multifunctional Hybrid Scaffold with Superparamagnetic Ability for Periodontal Regeneration

Mimicking Natural Microenvironments: Design of 3D-Aligned Hybrid Scaffold for Dentin Regeneration

3D Bioprinting of Lignocellulosic Biomaterials

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