Polymerase eta Is a Short-lived, Proteasomally Degraded Protein that Is Temporarily Stabilized Following UV Irradiation in Saccharomyces cerevisiae

Comparative analysis of in vivo interactions between Rev1 protein and other Y-family DNA polymerases in animals and yeasts

“…For UV treatment, cells were spun down and resuspended in water to OD 600 ~ 0.5, poured into large dishes to form a thin layer, then exposed to 50 J/m2 of UV (resulting in approximately 50% killing of WT). Irradiated cells were then resuspended in selective media with galactose and incubated at 30°C for 110-120 minutes after irradiation before harvesting, because previous work suggests that both Rev1 and Rad30 respond to DNA damage on this time scale [24][25][26][27]. Immunoprecipitations were performed essentially as described previously [23,28].…”

The Ubiquitin-Proteasome System and DNA Repair

“…Taken together, these results suggest that Rev3 and Rad30 are epistatic to Ump1 and that mutations caused by proteasomal defects depend upon both Pol and Pol (Podlaska et al, 2003;McIntyre et al, 2006). Rad30 is a short-lived protein post-translationally regulated by the UPS (Skoneczna et al, 2007). Its degradation depends on the Skp1/Cullin/F-box E3 enzyme complex and the Ufo1 F-box protein (Skoneczna et al, 2007).…”

“…Rad30 is a short-lived protein post-translationally regulated by the UPS (Skoneczna et al, 2007). Its degradation depends on the Skp1/Cullin/F-box E3 enzyme complex and the Ufo1 F-box protein (Skoneczna et al, 2007). Rev1 is also a relatively short-lived protein but has a longer half-life during G2/M-phases than G1-phase, potentially implicating proteasomal regulation.…”

REV7 is required for anaphase-promoting complex-dependent ubiquitination and degradation of translesion DNA polymerase REV1

“…42 Yeast and mammalian Polη can undergo proteasomal degradation. [43][44][45] Human Polλ, a DNA repair enzyme implicated in base excision repair, non-homologous end joining and TLS, is stabilized by phosphorylation, which prohibits proteasomal degradation in late S and G 2 phases. 46 Yeast REV1 mRNA and protein levels oscillate in a cell cycle-dependent manner.…”