Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis: A Simple Method for Species Identification in Food

Meyer, Rolf; Höfelein, C; Lüthy, Jürg; Candrian, U.

doi:10.1093/jaoac/78.6.1542

Cited by 240 publications

(88 citation statements)

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“…Similar results were reported by Ram et al (1996) for the restriction analysis of a 120 bp fragment of the cytochrome b in canned bonito species. Meyer et al (1995)…”

Section: Discussionmentioning

confidence: 99%

Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene

Céspedes

Garcı́a

Carrera

et al. 1998

Journal of Food Science

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Restriction site analysis of PCR products from a conserved region of the cytochrome b gene has been used for the specific identification of sole (Solea solea), European plaice (Pleuronectes platessa) and flounder (Platichthys flesus). Polymerase chain reaction (PCR) amplification of the cytochrome b gene using universal primers produced a 359 bp fragment in all species analyzed. Digestion of the PCR products with Nci I, Sau 3AI and Hinf I endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of the fish species. This methodology should prove useful for enforcing labeling regulations in the authentication of flatfish species.

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“…Similar results were reported by Ram et al (1996) for the restriction analysis of a 120 bp fragment of the cytochrome b in canned bonito species. Meyer et al (1995)…”

Section: Discussionmentioning

confidence: 99%

Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene

Céspedes

Garcı́a

Carrera

et al. 1998

Journal of Food Science

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“…The PCR primer set specific to the amplification of a 379-bp segment of the cry9C gene situated in the transgenic DNA was designed by Aventis CropScience. The PCR primer set specific to the amplification of a 108-bp segment of the growth hormone gene situated in the chromosomal DNA of swine was designed by Meyer et al (1995).…”

Section: (3) Detection Of the Cry9c Gene And The Cry9c Protein In Blomentioning

confidence: 99%

Tevaluation of transgenic event CBH 351 (StarLink) corn in pig

Yonemochi¹,

Suga²,

Harada³

et al. 2010

Animal Science Journal

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This study examined the influence of transgenic event CBH (StarLink; SL)-derived hybrid corn on growth, health and physiological functions of pigs, as well as the possibility of transferring the cry9C gene or Cry9C protein to the blood, liver or muscles, in comparison with pigs fed a diet with non-transgenic (isogenic) corn (non-SL). The diet for the SL group was composed of 70% SL corn, and the diet for the non-SL group was composed of 70% non-SL corn. Forty pigs approximately 3 months in age were used in the current experiment. After the pigs were acclimatized to their environment for 7 days, they were fed piglet diets for 7 weeks, and afterwards fed growing-finishing diets until the end of the experiment. There were no significant differences in bodyweight gain, feed intake or feed conversion ratio between the pigs fed SL diet and those of non-SL diet. No abnormalities were observed in the health conditions of either the SL or the non-SL group. Moreover, no significant differences were observed between the two groups in hematological values, histopathological examination and necropsy findings. Although the serum biochemical values within each group were normal, the blood urea nitrogen values of the SL group showed a tendency to be slightly higher than those of the non-SL group. Also, the blood glucose values of the SL group were significantly lower than those of the non-SL group. However, the cause of the significant differences in the blood glucose values between the two groups is unknown. The PCR and ELISA did not detect the cry9C gene and Cry9C protein in the blood, liver or muscles of the pigs at the end of the experiment.

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“…However, all such methods rely upon sequencing PCR products, which is costly and time consuming. An alternative approach to sequencing is the analysis of PCR fragments with endonucleases to detect interspecific restriction fragment length polymorphisms (RFLPs) (Meyer et al, 1995;Ram et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

Salmon and Trout Analysis by PCR‐RFLP for Identity Authentication

Carrera

Garcı́a

Céspedes

et al. 1999

Journal of Food Science

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Raw and smoked Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) were differentiated by PCR amplification of a 950 bp conserved fragment of the mitochondrial 16S rRNA gene followed by restriction site analysis with the endonucleases Hpa I and Bst EII. Salmon PCR products digested with Hpa I yielded two fragments of 804 bp and 146 bp, while trout PCR products were not cleaved by this enzyme. However, Bst EII did not cleave salmon PCR products while two bands of 513 bp and 437 bp were produced when trout samples were cleaved with this enzyme. This genetic marker could be very useful for detecting fraudulent substitution of lower valued smoked trout.

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Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis: A Simple Method for Species Identification in Food

Cited by 240 publications

References 0 publications

Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene

Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene

Tevaluation of transgenic event CBH 351 (StarLink) corn in pig

Salmon and Trout Analysis by PCR‐RFLP for Identity Authentication

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