Polymerase Chain Reaction(PCR) for the Detection of Herpesvirus in Tortoises.

Une, Yumi; Murakami, Masaru; Uemura, Kayoko; Fujitani, Hideo; Ishibashi, Tôru; Nomura, Yasuo

doi:10.1292/jvms.62.905

Cited by 29 publications

(24 citation statements)

References 5 publications

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“…The sequence information available is, however, generally limited to a small portion of the DNA polymerase gene. Analysis of this short fragment supports the classification of chelonian HVs in the subfamily Alphaherpesvirinae of the family Herpesviridae [57,61,62,66–69]. It has been suggested that the chelonian HVs form a monophyletic group typical of other genera in the family Herpesviridae , and that these viruses be classified together in a new genus with the suggested name “Chelonivirus” [57].…”

Section: Herpesviridaesupporting

confidence: 60%

“…A revised method of naming newly described and characterized chelonian HVs, particularly HVs of tortoises, has therefore been used in recent publications. The first tortoise HV from which sequence data from a small portion of the DNA polymerase gene became available, allowing a preliminary taxonomic analysis of the isolate, was from Russian and pancake tortoises ( Testudo horsfieldii and Malacochersus tornieri ) in Japan [69]. Studies on HVs from European tortoises showed that similar viruses can also be found in Russian tortoises in Europe, but that these viruses differ from the majority of isolates found in Mediterranean tortoises kept as pets in Europe, building two distinct genogroups [62].…”

Section: Herpesviridaementioning

confidence: 99%

“…TeHV1 has mainly been described in Russian tortoises, but can also infect several other species. It is generally associated with relatively low morbidity and mortality rates [69,75]. TeHV3 has been detected in many different tortoise species, most commonly in spur-thighed tortoises ( T. graeca ), marginated tortoises ( T. marginata ), Hermann’s tortoises ( T. hermanni ), and Russian tortoises ( T. horsfieldii ).…”

Section: Herpesviridaementioning

confidence: 99%

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Viruses Infecting Reptiles

Marschang

2011

Viruses

181

165

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A large number of viruses have been described in many different reptiles. These viruses include arboviruses that primarily infect mammals or birds as well as viruses that are specific for reptiles. Interest in arboviruses infecting reptiles has mainly focused on the role reptiles may play in the epidemiology of these viruses, especially over winter. Interest in reptile specific viruses has concentrated on both their importance for reptile medicine as well as virus taxonomy and evolution. The impact of many viral infections on reptile health is not known. Koch’s postulates have only been fulfilled for a limited number of reptilian viruses. As diagnostic testing becomes more sensitive, multiple infections with various viruses and other infectious agents are also being detected. In most cases the interactions between these different agents are not known. This review provides an update on viruses described in reptiles, the animal species in which they have been detected, and what is known about their taxonomic positions.

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Section: Herpesviridaesupporting

confidence: 60%

Section: Herpesviridaementioning

confidence: 99%

Section: Herpesviridaementioning

confidence: 99%

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Viruses Infecting Reptiles

Marschang

2011

Viruses

181

165

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“…9,20,21,24,[31][32][33] PCR likewise has proven to be a powerful tool for the retrospective detection of viral nucleic acid in paraffinized brain tissues. 12,24,32,[34][35][36] In this study, we used degenerate herpes-and adenoviral PCR and a specific canine parvovirus PCR to study paraffinized brain tissues from dogs that died from NME, NLE, or GME.…”

Section: Discussionmentioning

confidence: 99%

Polymerase Chain Reaction Screening for DNA Viruses in Paraffin-Embedded Brains from Dogs with Necrotizing Meningoencephalitis, Necrotizing Leukoencephalitis, and Granulomatous Meningoencephalitis

Schatzberg¹,

Haley²,

Barr³

et al. 2005

J Vet Int Med

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The objective of this investigation was to determine whether or not herpesvirus (herpes-), adenovirus (adeno-), or canine parvovirus DNA is present in the brains of dogs with necrotizing meningoencephalitis (NME), necrotizing leukoencephalitis (NLE), and granulomatous meningoencephalitis (GME). Paraffin-embedded brain specimens from 12 histopathologically confirmed dogs with NME, 3 with NLE, and 7 with GME were screened for viral DNA with degenerate herpes- and adenovirus polymerase chain reaction (PCR) and a canine parvovirus-specific PCR. Positive-control specimens included genomic viral DNA and paraffin-embedded tissues from dogs with confirmed herpes-, adeno-, or canine parvovirus infections. Herpes-, adeno-, or canine parvovirus DNA was amplified by PCR from the corresponding positive-control specimens. Negative controls included 7 dogs with various brain disorders and produced no viral amplicons. The 22 dogs with NME, NLE, and GME were negative for viral DNA. Additional studies testing for other viruses or inherited genetic mutations are warranted to gain insight into the etiologies of NME, NLE, and GME. We discuss potential etiologies and provide a clinical and histopathologic overview of these common canine encephalitides.

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“…An 181-bp region obtained from the ToHV using nested PCR was further analyzed by nucleotide sequencing and indicated that this tortoise virus is a new type of her- pesvirus. 12 However, such a small region of sequence data was not always sufficient for characterization of the virus. Hence, the aim of this study was to develop a new PCR diagnostic technique specific to the ToHV.…”

mentioning

confidence: 99%

Development of Species-Specific PCR Techniques for the Detection of Tortoise Herpesvirus

et al. 2001

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Previously, a nested polymerase chain reaction (PCR) was employed with consensus degenerate primers targeting highly conserved motifs within herpesviral DNA polymerase genes to detect a newly described tortoise herpesvirus. However, nucleotide sequence information obtained from the final amplified fragment was restricted to a small region of 181 bp. In the present study, additional sequences flanking this segment were determined from a PCR product successfully amplified using a set of known degenerate primers, which covered a 692-bp region within the tortoise herpesviral DNA polymerase gene. Polymerase chain reaction primers for specific amplification of the tortoise herpesviral DNA were designed on the basis of these nucleotide sequences and successfully amplified tortoise herpesviral DNA from the tissues of tortoises that were well characterized histopathologically with herpesviral infection. The lower limit of detection was 1,000 herpesviral DNA equivalents in the presence of normal tortoise genomic DNA. Furthermore, a more sensitive and specific PCR technique for the identification of herpesviral infections in tortoises was developed employing a heminested form, which will enable the detection of latent infections or herpesvirus carriers in tortoises.

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Polymerase Chain Reaction(PCR) for the Detection of Herpesvirus in Tortoises.

Cited by 29 publications

References 5 publications

Viruses Infecting Reptiles

Viruses Infecting Reptiles

Polymerase Chain Reaction Screening for DNA Viruses in Paraffin-Embedded Brains from Dogs with Necrotizing Meningoencephalitis, Necrotizing Leukoencephalitis, and Granulomatous Meningoencephalitis

Development of Species-Specific PCR Techniques for the Detection of Tortoise Herpesvirus

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