Polymerase chain reaction (PCR)-based molecular discrimination between Paragonimus westermani and P. miyazakii at the metacercarial stage

Sugiyama, Hiromu; Morishima, Yasuyuki; Kameoka, Yosuke; Kawanaka, Masanori

doi:10.1006/mcpr.2002.0417

Cited by 31 publications

(20 citation statements)

References 16 publications

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“…22,23 Prior studies have used molecular diagnostics to speciate Paragonimus metacerariae in snails. [24][25][26] However, relatively few studies have attempted to detect Paragonimus DNA in the definitive host. [27][28][29] Our PCR studies confirmed the presence of P. kellicotti DNA in clinical specimens from two Missouri cases.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Fischer¹,

Curtis²,

Marcos³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Fischer¹,

Curtis²,

Marcos³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

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“…Species-specific PCR assays have been constructed for the detection of some Paragonimus species (175). Likely more useful are broad-range PCR assays that target conserved regions within the Paragonimus genome.…”

Section: Molecular Testingmentioning

confidence: 99%

North American Paragonimiasis (Caused by Paragonimus kellicotti ) in the Context of Global Paragonimiasis

2009

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SUMMARY Paragonimus species are highly evolved parasites with a complex life cycle that involves at least three different hosts, i.e., snails, crustaceans, and mammals. The adult forms of Paragonimus species reside and mate in the lungs of a variety of permissive mammalian hosts, including humans. Although human paragonimiasis is uncommonly encountered in North America, both autochthonous and imported disease may be encountered. Paragonimus kellicotti, the species endemic to North America, is a well-known pathogen in wild and domestic animals. Five patients with North American paragonimiasis have been reported in the recent medical literature. The biologic, clinical, radiologic, and laboratory features of paragonimiasis are reviewed, with emphasis on North American paragonimiasis whenever possible.

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“…Las metacercarias de Paragonimus, a su vez, constituyen una oportunidad para adelantar estudios moleculares en taxonomía e inmunodiagnóstico, alternativas que se trabajan en los países donde existe una gran prevalencia de paragonimosis (20)(21)(22)(23).…”

Section: Discussionunclassified

Distribución parcial de Paragonimus (Digenea: Troglotrematidae) en Antioquia, por presencia de metacercarias en cangrejos dulciacuícolas

2008

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Introducción. La paragonimosis, o distomatosis pulmonar, es una enfermedad con sintomatología similar a la observada en la tuberculosis. Es causada por parásitos del género Paragonimus (Digenea: Troglotrematidae). Las personas se infectan al consumir cangrejos crudos o mal cocidos, con metacercarias del parásito. El primer foco de paragonimosis humana en Colombia se registró durante 1995 en Urrao, Antioquia, donde se hallaron dos especies de cangrejos que hospedaban el parásito. En el 2005 se capturaron cangrejos con metacercarias de Paragonimus en Medellín, lo que motivó la búsqueda del parásito en otras localidades, mediante su presencia en estos crustáceos. Objetivo. Establecer la distribución de Paragonimus en Antioquia, evaluando la presencia de metacercarias en macrocrustáceos braquiuros, dulciacuícolas. Materiales y métodos. Desde 2005 hasta 2007 se capturaron cangrejos en 13 municipios antioqueños. Se relajaron y sacrificaron para la búsqueda del digeneo y la identificación taxonómica. Resultados. En nueve municipios se capturaron 52 cangrejos, 42 (80,76%) con metacercarias de Paragonimus. Todos los crustáceos se determinaron como Pseudothelphusidae, de los géneros Hypolobocera y Strengeriana, y se asignaron a cuatro especies. Tres se registran por primera vez como huéspedes del parásito. Conclusión. Se inicia la construcción de un mapa con la distribución de Paragonimus en Antioquia que incluye por primera vez zonas urbanizadas. Se ratifican el consumo y la manipulación de los cangrejos crudos y mal cocidos como factores de riesgo para la infección humana. Se propone a los cangrejos como agentes focalizadores de paragonimosis por ser asequibles y de fácil diagnóstico.Palabras clave: braquiuros, Colombia, interacciones huésped-parásitos, localización geográfica de riesgo, Paragonimus, trematodos. Results. From 52 crabs captured in 9 counties, 42 (80.8%) were found with Paragonimus metacercariae. The crabs were identified as Pseudothelphusidae in 2 genera-Hypolobocera and Strengeriana-and were assigned to four species. Three of the species were recorded for the first time as hosts of Paragonimus. Conclusions. A Paragonimus' distribution map was constructed for Antioquia; for the first time urban zones were included. Because of the high rate of infection, the handling and consumption of raw and poorly cooked crabs pose risk factors for human infection. Because crabs are affordable and provide means of easy diagnosis, crabs are targeted as primary agents of and diagnostic tools for paragonmosis. Distribution of

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Polymerase chain reaction (PCR)-based molecular discrimination between Paragonimus westermani and P. miyazakii at the metacercarial stage

Cited by 31 publications

References 16 publications

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

North American Paragonimiasis (Caused by Paragonimus kellicotti ) in the Context of Global Paragonimiasis

Distribución parcial de Paragonimus (Digenea: Troglotrematidae) en Antioquia, por presencia de metacercarias en cangrejos dulciacuícolas

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