Polymerase chain reaction-mediated DNA fingerprinting for epidemiological studies on Campylobacter spp.

Giesendorf, B. A. J.; Goossens, Herman; Niesters, H. G. M.; Belkum, Alex van; Koeken, Ankie; Endtz, Hubert P.; Stegeman, H.; Quint, W. G. V.

doi:10.1099/00222615-40-2-141

Cited by 31 publications

(21 citation statements)

References 2 publications

(3 reference statements)

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“…These sequences are highly conserved and are able to show discrimination in many other organisms [33,34]. In the present study, variations within the typable strains were observed by AP1 primer PCR fingerprinting.…”

Section: Discussionmentioning

confidence: 73%

Subtype distribution of Haemophilus influenzae isolates from North India

Sharma

Kaur

Ganguly

et al. 2002

Journal of Medical Microbiology

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A total of 120 Haemophilus influenzae isolates from blood, cerebrospinal fluid, sputum and throat swabs of patients and carriers in North India was characterised by biotyping, ribotyping and random amplification of polymorphic DNA (RAPD)-PCR. Of these, 77 isolates (64%) were serotype b; the other 43 (36%) were non-typable. Biotype I was the most predominant among the typable strains and biotype II among the non-typable strains. Ribotyping with restriction endonucleases HaeIII and EcoRI differentiated the isolates into three and six ribotypes, respectively. However, RAPD fingerprints generated by the application of arbitrary primers AP1 and AP2 provided a higher level of discrimination. RAPD typing revealed distinct polymorphism among the serologically typable isolates. This study is the first report that stratifies the subtypes of H. influenzae strains from India by molecular techniques.

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Section: Discussionmentioning

confidence: 73%

Subtype distribution of Haemophilus influenzae isolates from North India

Sharma

Kaur

Ganguly

et al. 2002

Journal of Medical Microbiology

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“…ERIC-PCR was performed with the sequences ERIC1R and ERIC2 (47), using a Perkin-Elmer thermal cycler model 480. After the PCR was carried out, the PCR products were separated on 1.5% agarose gels and stained with ethidium bromide (6). Digitized images of the gels (tagged image file format) were obtained (One-scanner; Apple), and banding patterns were detected by using the RestrictoScan and RestrictoTyper programs from the Taxotron package (Taxolab, Institut Pasteur, Paris, France).…”

Section: Methodsmentioning

confidence: 99%

“…The reproducibility and discriminatory power of the ERIC-PCR technique in the typing of thermophilic Campylobacter spp. favors its application in epidemiological studies (6,42,48,49).…”

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confidence: 99%

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Mouwen

Weijtens

Capita

et al. 2005

Appl Environ Microbiol

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Fourier transform infrared spectroscopy (FT-IR) has been used together with pattern recognition methodology to study isolates belonging to the species Campylobacter coli and Campylobacter jejuni and to compare FT-IR typing schemes with established genomic profiles based on enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Seventeen isolates were cultivated under standardized conditions for 2, 3, and 4 days to study variability and improve reproducibility. ERIC-PCR profiles and FT-IR spectra were obtained from strains belonging to the species Campylobacter coli and C. jejuni, normalized, and explored by hierarchical clustering and stepwise discriminant analysis. Strains could be differentiated by using mainly the firstderivative FT-IR spectral range, 1,200 to 900 cm ؊1 (described as the carbohydrate region). The reproducibility index varied depending on the ages of the cultures and on the spectral ranges investigated. Classification obtained by FT-IR spectroscopy provided valuable taxonomic information and was mostly in agreement with data from the genotypic method, ERIC-PCR. The classification functions obtained from the discriminant analysis allowed the identification of 98.72% of isolates from the validation set. FT-IR can serve as a valuable tool in the classification, identification, and typing of thermophilic Campylobacter isolates, and a number of types can be differentiated by means of FT-IR spectroscopy.

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“…Plasmids were purified from E. coli by alkaline lysis or with the QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany). Chromosomal Campylobacter DNA was isolated either using a lysozyme lysis method (29) or with cetyl trimethyl ammonium bromide (CTAB) (4).…”

Section: Bacterial Strains Growth Conditions and Identification Of mentioning

confidence: 99%

A Genomic Island Defines Subspecies-Specific Virulence Features of the Host-Adapted Pathogen Campylobacter fetus subsp. venerealis

et al. 2010

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The pathogen Campylobacter fetus comprises two subspecies, C. fetus subsp. fetus and C. fetus subsp. venerealis. Although these taxa are highly related on the genome level, they are adapted to distinct hosts and tissues. C. fetus subsp. fetus infects a diversity of hosts, including humans, and colonizes the gastrointestinal tract. In contrast, C. fetus subsp. venerealis is largely restricted to the bovine genital tract, causing epidemic abortion in these animals. In light of their close genetic relatedness, the specific niche preferences make the C. fetus subspecies an ideal model system to investigate the molecular basis of host adaptation. In this study, a subtractive-hybridization approach was applied to the genomes of the subspecies to identify different genes potentially underlying this specificity. The comparison revealed a genomic island uniquely present in C. fetus subsp. venerealis that harbors several genes indicative of horizontal transfer and that encodes the core components necessary for bacterial type IV secretion. Macromolecular transporters of this type deliver effector molecules to host cells, thereby contributing to virulence in various pathogens. Mutational inactivation of the putative secretion system confirmed its involvement in the pathogenicity of C. fetus subsp. venerealis.

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Polymerase chain reaction-mediated DNA fingerprinting for epidemiological studies on Campylobacter spp.

Cited by 31 publications

References 2 publications

Subtype distribution of Haemophilus influenzae isolates from North India

Subtype distribution of Haemophilus influenzae isolates from North India

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

A Genomic Island Defines Subspecies-Specific Virulence Features of the Host-Adapted Pathogen Campylobacter fetus subsp. venerealis

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