Polymerase Chain Reaction Identification of Three Members of the<i>Anopheles sundaicus</i>(Diptera: Culicidae) Complex, Malaria Vectors in Southeast Asia

Dusfour, Isabelle; Blondeau, Johanna; Harbach, Ralph E.; Vythilingham, Indra; Baimai, Visut; Trung, Ho Dinh; Sochanta, Tho; Bangs, Michael J.; Manguin, Sylvie

doi:10.1093/jmedent/44.5.723

Cited by 34 publications

(19 citation statements)

References 30 publications

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“…However, this is contradictory with more current findings based on DNA evidence indicating that An . epiroticus appears confined to mainland Southeast Asia and not present in Indonesia or eastern Malaysia (Borneo) [ 20 , 22 , 23 ]. Form B has been found in sympatry with Form A at inland freshwater sites near Purworejo (south-central Java), and South Tapanuli (northern Sumatra).…”

Section: Introductionmentioning

confidence: 99%

“…Until very recently, An . sundaicus species E in Indonesia (e.g., Sumatra, Java and Sumba islands) was considered allopatric with the other Sundaicus Complex members with separation based on COI sequences using a multiplex allele-specific polymerase chain reaction [ 20 , 22 , 25 – 27 ]. So far, the relationships of cytotypes B and C to other members in the complex remain unclear [ 22 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Anopheles sundaicus complex and the presence of Anopheles epiroticus in Indonesia

et al. 2020

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Anopheles sundaicus s.l. is an important malaria vector primarily found in coastal landscapes of western and central Indonesia. The species complex has a wide geographical distribution in South and Southeast Asia and exhibits ecological and behavioural variability over its range. Studies on understanding the distribution of different members in the complex and their bionomics related to malaria transmission might be important guiding more effective vector intervention strategies. Female An. sundaicus s.l. were collected from seven provinces, 12

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anopheles sundaicus complex and the presence of Anopheles epiroticus in Indonesia

et al. 2020

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“…Small variations in ITS2 and COI sequences are strong indicators of distinct species. The majority of species-specific PCRs used to identify Anopheles and other cryptic species based on minor sequence variations within these regions 26 30 31 34 . ITS2 sequences from this study are available in GenBank with accession numbers KR014818 – KR014835.…”

Section: Resultsmentioning

confidence: 99%

Unexpected diversity of Anopheles species in Eastern Zambia: implications for evaluating vector behavior and interventions using molecular tools

Lobo

Laurent

Sikaala³

et al. 2015

Sci Rep

117

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The understanding of malaria vector species in association with their bionomic traits is vital for targeting malaria interventions and measuring effectiveness. Many entomological studies rely on morphological identification of mosquitoes, limiting recognition to visually distinct species/species groups. Anopheles species assignments based on ribosomal DNA ITS2 and mitochondrial DNA COI were compared to morphological identifications from Luangwa and Nyimba districts in Zambia. The comparison of morphological and molecular identifications determined that interpretations of species compositions, insecticide resistance assays, host preference studies, trap efficacy, and Plasmodium infections were incorrect when using morphological identification alone. Morphological identifications recognized eight Anopheles species while 18 distinct sequence groups or species were identified from molecular analyses. Of these 18, seven could not be identified through comparison to published sequences. Twelve of 18 molecularly identified species (including unidentifiable species and species not thought to be vectors) were found by PCR to carry Plasmodium sporozoites - compared to four of eight morphological species. Up to 15% of morphologically identified Anopheles funestus mosquitoes in insecticide resistance tests were found to be other species molecularly. The comprehension of primary and secondary malaria vectors and bionomic characteristics that impact malaria transmission and intervention effectiveness are fundamental in achieving malaria elimination.

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“…While it would appear that An. epiroticus is most likely the species present in coastal areas of Bangladesh, due to the limited geographic provenance of the other species, this awaits confirmation using molecular methods [ 132 ]. Anopheles annularis is a complex of two species, A and B [ 133 ], which can be distinguished using chromosomal or molecular methods [ 134 ].…”

Section: Discussionmentioning

confidence: 99%

A review of the mosquito species (Diptera: Culicidae) of Bangladesh

et al. 2016

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BackgroundDiseases caused by mosquito-borne pathogens remain an important source of morbidity and mortality in Bangladesh. To better control the vectors that transmit the agents of disease, and hence the diseases they cause, and to appreciate the diversity of the family Culicidae, it is important to have an up-to-date list of the species present in the country. Original records were collected from a literature review to compile a list of the species recorded in Bangladesh.ResultsRecords for 123 species were collected, although some species had only a single record. This is an increase of ten species over the most recent complete list, compiled nearly 30 years ago. Collection records of three additional species are included here: Anopheles pseudowillmori, Armigeres malayi and Mimomyia luzonensis. ConclusionsWhile this work constitutes the most complete list of mosquito species collected in Bangladesh, further work is needed to refine this list and understand the distributions of those species within the country. Improved morphological and molecular methods of identification will allow the refinement of this list in years to come.

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Polymerase Chain Reaction Identification of Three Members of theAnopheles sundaicus(Diptera: Culicidae) Complex, Malaria Vectors in Southeast Asia

Cited by 34 publications

References 30 publications

Anopheles sundaicus complex and the presence of Anopheles epiroticus in Indonesia

Anopheles sundaicus complex and the presence of Anopheles epiroticus in Indonesia

Unexpected diversity of Anopheles species in Eastern Zambia: implications for evaluating vector behavior and interventions using molecular tools

A review of the mosquito species (Diptera: Culicidae) of Bangladesh

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