Polymerase Chain Reaction Identification of Microorganisms in Previously Root-Filled Teeth in a South Korean Population

Enterococcus Spp. in Endodontically Treated Teeth with and without Periradicular Lesions

“…in the root canals of cases receiving endodontic retreatment was 12.1%. This prevalence is lower than that previously reported in some studies 64-77% (Rocas and Jung 2004; Siqueira Junior 2004) using PCR identification, but is consistent with the 22% reported in an earlier study by our group (Fouad and Barry 2005). In fact an earlier molecular study (Rolph et al 2001), and one culture study (Cheung and Ho 2001 ) failed to identify enterococci in retreatment cases.…”

Multiplex Polymerase Chain Reaction Detection of Black-Pigmented Bacteria in Infections of Endodontic Origin

“…Ng et al (17) recommended 2.5% sodium hypochlorite instead of iodine tincture before root canal sampling for PCR. In this study, 3% hydrogen peroxide and 2.5% sodium hypochlorite were used for decontamination like Rocas et al (20,21) and Siqueira et al (26,27). Ng et al (17) also recommended a second disinfecting with 2.5% sodium hypochlorite after access cavity preparation, but in this study the operation field was disinfected secondly just before penetrating the roof because of preserving the microbes in the pulp chamber for conventional culture procedures.…”

Comparative Analysis of Enterococcus faecalis Biofilm Formation on Different Substrates

“…Enterococcus faecalis are facultative anaerobic gram-positive bacteria frequently isolated from root canals in cases of endodontic treatment failure (2)(3)(4). Resistant endodontic microbiota includes bacteria capable of adhesion and biofilm formation.…”