Polymerase Chain Reaction for Phytoplasmas Detection

Delić, Duška

doi:10.5772/37728

Cited by 8 publications

(14 citation statements)

References 124 publications

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“…Khan et al (2008) revealed, following restriction fragment length polymorphism (RFLP) analysis, and using nested PCR of 16S rDNA (Khan et al 2004), that two SSD strains showed high sequence similarity to the aster yellows subgroup 16SrI-B. PCR-based detection of phytoplasmas has been reviewed by Delić (2012). PCR-based methods revealed that Ca.…”

Section: Detectionmentioning

confidence: 99%

“…Methods based on ELISA and electron microscopy were reported by Balasundaran and Muralidharan (2004) and Thomas and Balasundaran (2001). ELISA could detect SSD six months before the expression of external symptoms (Balasundaran, Muralidharan 2004) but serological methods are insufficiently sensitive to detect various phytoplasmas (Delić 2012). PCR-based methods were used by Khan et al (2004; and Murli et al (2013).…”

Section: Detectionmentioning

confidence: 99%

“…PCR-based methods revealed that Ca. Phytoplasma cynodontis belongs to 16SrI, which is one of the major groups of phytoplasmas associated with different plant species reported to be infected with phytoplasmas worldwide (Lee et al 1998;Hogenhout et al 2008;Bertaccini, Duduk 2009;Laimer et al 2009;Marcone 2011;Rao et al 2011;Delić 2012;Kube et al 2012;Gurr et al 2016). An understanding of phytoplasma ecology and epidemiology has increased as a result of the use of these PCR-based methods (Martin et al 2000).…”

Section: Detectionmentioning

confidence: 99%

“…At the time when phytoplasmas were discovered, the diagnosis of plant pathogens was difficult since detection was based on the "observation of symptoms, insect or dodder/ graft transmission to host plant and electron microscopy of ultra-thin sections of the phloem tissue" (Bertaccini, Duduk 2009;Delić 2012). Serological diagnostic techniques for the detection of phytoplasmas began to emerge in the 1980's with direct and indirect enzyme-linked immunosorbent assay (ELISA)-based methods (Martin et al 2000;Delić 2012).…”

Section: Detectionmentioning

confidence: 99%

“…Serological diagnostic techniques for the detection of phytoplasmas began to emerge in the 1980's with direct and indirect enzyme-linked immunosorbent assay (ELISA)-based methods (Martin et al 2000;Delić 2012). Methods based on ELISA and electron microscopy were reported by Balasundaran and Muralidharan (2004) and Thomas and Balasundaran (2001).…”

Section: Detectionmentioning

confidence: 99%

See 4 more Smart Citations

Sandalwood spike disease: a brief synthesis

Silva¹

2016

EEB

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Sandalwood spike disease (SSD) is likely to be the most destructive of known diseases to infect Indian sandalwood, Santalum album L., which is valued for its wood and scented essential oil derived from the heartwood. Even though the history of research on SSD extends for well over a century, most of the earlier work was primarily descriptive in macromorphological aspects. In 1969, a breakthrough was achieved by three independent research groups that confirmed the causal agent of SSD to be a phytoplasma. Despite this discovery, the identity of the causal agent was only confirmed by a single group using RFLP analysis and nested PCR of 16S rDNA. The phytoplasma that was identified showed high sequence similarity to aster yellows subgroup 16SrI-B. Tissue culture to derive disease-free clones and transgenic strategies to derive disease-resistant trees may be the only realistic solutions for ridding the limited S. album forests of SSD. This brief synthesis aims to stimulate greater interest in SSD, especially since sandalwood production has expanded to the Chinese mainland and to Australia.

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Section: Detectionmentioning

confidence: 99%

Section: Detectionmentioning

confidence: 99%

Section: Detectionmentioning

confidence: 99%

Section: Detectionmentioning

confidence: 99%

Section: Detectionmentioning

confidence: 99%

See 3 more Smart Citations

Sandalwood spike disease: a brief synthesis

Silva¹

2016

EEB

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Detection, Identification, and Characterization of Phytoplasmas Infecting Apple and Pear Trees in Belarus

Valasevich

Schneider

2016

Plant Disease

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In Samochvalovichi, Belarus, apple and pear tree root samples were examined for the presence of phytoplasmas using a universal 16S rDNA-based PCR assay. Out of 27 tested apple trees, 23 were found to be infected by ‘Candidatus Phytoplasma mali’ and 46 out of 58 pear trees were positive for the presence of ‘Ca. P. pyri.’ Species were identified by sequence analysis of the 16S rDNA amplicons. The molecular diversity of the phytoplasma isolates was examined by analysis of an hflB gene using single-strand conformation polymorphism (SSCP) and sequence analysis. Therefore hflB gene amplicons from ‘Ca. P. mali’ and ‘Ca. P. pyri’ accessions were cloned after amplification. Screening of 640 cloned hflB fragments by SSCP analysis revealed the presence of eight different profiles for ‘Ca. P. mali’ and 12 different profiles for ‘Ca. P. pyri.’ The variants were sequenced and compared in multiple alignments. The nucleic acid homology among the hflB gene fragments ranged between 95.4 and 100.0% and 81.3 to 100.0% for ‘Ca. P. mali’ and ‘Ca. P. pyri,’ respectively, indicating a high genetic variability within the species. This is the first report on the occurrence of ‘Ca. P. mali’ and ‘Ca. P. pyri’ in Belarusian apple and pear trees and their molecular diversity.

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A critical appraisal on the recurrence of sandalwood spike disease and its management practices

2020

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Sandal (Santalum album) is an important forest tree that has been utilized for centuries, valued for its wood and scented essential oil derived from the heartwood. Sandalwood spike disease (SSD) is one of the most destructive problems that affect Indian sandalwood. In 1969, three research groups confirmed that the causal agent of SSD was a phytoplasma. Although research literature on sandalwood spike disease has been published for over a century, many earlier publications reported only primarily descriptive macro‐morphological data. Identification of the causal agent was confirmed only recently, using nested PCR of 16S rRNA‐encoding gene sequences and restriction fragment length polymorphism (RFLP) analysis. The identified phytoplasma exhibited high sequence similarity to the aster yellows subgroup 16SrI‐B in NCBI BLAST search. The use of tissue culture in transgenic and disease‐free cloning strategies to produce disease‐resistant sandalwood tress might be the most realistic solution for SSD. The aim of this review is to increase understanding of SSD among phytopathologists in order to encourage research on SSD to discover best management practices. The increased cultivation of sandalwood plantations has significant importance, and it is essential to review the status of SSD.

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Polymerase Chain Reaction for Phytoplasmas Detection

Cited by 8 publications

References 124 publications

Sandalwood spike disease: a brief synthesis

Sandalwood spike disease: a brief synthesis

Detection, Identification, and Characterization of Phytoplasmas Infecting Apple and Pear Trees in Belarus

A critical appraisal on the recurrence of sandalwood spike disease and its management practices

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