Polymerase chain reaction for detection of <i>Mycobacterium tuberculosis</i>

Narita, Mitsuo; Shibata, Mutsuo; Togashi, Takehiro; Kobayashi, Hiroaki

doi:10.1111/j.1651-2227.1992.tb12190.x

Cited by 21 publications

(11 citation statements)

References 16 publications

(1 reference statement)

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“…A PCR na detecção de micobactérias não é à prova de falhas, pois sua sensibilidade na detecção de micobactérias tem sido relatada na faixa de 55-100% [28][29][30][31] . Por outro lado, esta investigação pode ser usada quando a carga bacteriana é muito baixa 15,32 .…”

Section: Discussionunclassified

Children with significant cervical lymphadenopathy: clinicopathological analysis and role of fine-needle aspiration in Indian setup

Khan¹,

Wahab²,

Chana³

et al. 2008

J Pediatr (Rio J)

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ResumoObjetivo: Estudar o perfil clínico-patológico de crianças indianas com linfadenopatia cervical e o papel da citologia aspirativa por agulha fina com ênfase especial na tuberculose como causa. Resultados: A hiperplasia reativa foi o tipo mais comum de linfadenite, seguida da granulomatosa. Os linfonodos do triângulo posterior unilateral foram o grupo afetado com maior freqüência no grupo de linfadenopatia cervical tuberculosa. A aspiração por agulha fina, seguida da coloração de Ziehl-Neelsen, histopatologia e cultura em associação, obteve sucesso em realizar o diagnóstico em 85,7% dos casos de etiologia tuberculosa. Métodos Conclusões:A aspiração por agulha fina é uma ferramenta diagnóstica valiosa no tratamento de crianças com apresentação clínica de linfonodos cervicais aumentados. A técnica reduz a necessidade de procedimentos mais invasivos e dispendiosos, principalmente em países em desenvolvimento.Cultura e histopatologia, entretanto, devem ser consideradas em casos nos quais a citologia aspirativa por agulha fina não é diagnóstica.J Pediatr (Rio J). 2008;84(5):449-454: Linfadenopatia cervical, citologia aspirativa por agulha fina, crianças. AbstractObjective: To study the clinicopathological profile of children from India with cervical lymphadenopathy and the role of fine-needle aspiration cytology with special emphasis on tuberculosis as a cause. Methods:A total of 89 children in the age group of 10 months to 12 years, presenting to our hospital from April 2004 to March 2005, were included. All the patients underwent thorough clinical and investigational assessment vis-à-vis cervical lymphadenopathy. Outcome measurements included clinical status and ability of conventional tests to categorize different types of lymphadenopathy and their utility in diagnosing tubercular lymphadenitis. Interobserver variability was analyzed measuring kappa test and was found to be in agreement. Results:Reactive hyperplasia was the most common type of lymphadenitis, followed by granulomatous involvement. Unilateral posterior triangle lymph nodes were the most commonly affected in the tubercular cervical lymphadenopathy group. Fine-needle aspiration followed by Ziehl-Neelsen staining, histopathology and culture in combination were able to perform the diagnosis in 85.7% of cases affected with tubercular etiology. Conclusions:Fine-needle aspiration is a valuable diagnostic tool in the management of children with the clinical presentation of enlarged cervical lymph nodes. The technique reduces the need for more invasive and costly procedures, especially in a Third World country. Culture and histopathology, however, should be considered in cases where repeated fine-needle aspiration cytology is non-diagnostic.J Pediatr (Rio J). 2008;84(5):449-454: Cervical lymphadenopathy, fine-needle aspiration cytology, children.

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Section: Discussionunclassified

Children with significant cervical lymphadenopathy: clinicopathological analysis and role of fine-needle aspiration in Indian setup

Khan¹,

Wahab²,

Chana³

et al. 2008

J Pediatr (Rio J)

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“…The use of fine-needle aspirations in patients without HIV infections is highly variable [8]. A polymerase chain reaction for Mycobacterium tuberculosis of the fine needle aspiration specimen enhances test sensitivity [11]. …”

Section: Discussionmentioning

confidence: 99%

Tuberculous disseminated lymphadenopathy in an immunocompetent non-HIV patient: a case report

et al. 2009

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IntroductionIn cases of patients with disseminated lymphadenopathy, the differential diagnosis has to include both benign and malignant causes, including sarcoidosis, metastatic disease, lymphoma and, although rarely present, tuberculosis. Tuberculosis is still one of the most frequently occurring infectious diseases worldwide. However, disseminated mycobacterial lymphadenitis is rare in immunocompetent patients.Case presentationWe present the case of a 56-year-old Caucasian Greek male, who was immunocompetent and HIV negative, with a two-month history of recurring fever, loss of appetite and disseminated lymphadenopathy. The patient was diagnosed with mycobacterial lymphadenopathy.ConclusionThis case highlights the need for suspicion in order to identify mycobacterial infection in patients with generalized lymphadenopathy, since misdiagnosis is possible and may lead to fatal complications for the patient.

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“…We consider 10 fg to be sufficient for practical purposes. This implied that under experimental conditions, at least two tuberculous bacilli being lysed was sufficient for obtaining a positive result and that this was not very different from that of the first step amplification (9).…”

Section: Sensitivitymentioning

confidence: 92%

“…In our previous report, we described the successful detection of Mycobacterium tuberculosis in two patients with tuberculous lymphadenitis using polymerase chain reaction (PCR) (9). However, during the course of the successive study, we found it necessary to devise a more sensitive assay system as well as a standard methodology for sample preparation and for amplification which would be applicable to various forms of clinical samples.…”

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confidence: 99%

Nested amplification protocol for the detection of Mycobacterium tuberculosis

et al. 1992

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Several methods for rapid diagnosis of tuberculosis have been devised through DNA amplification. However, the chemically strong cell wall of the species, the presumptively low numbers of organisms and their uneven distribution in clinical samples, and the lack of a "gold standard" for diagnosing tuberculosis, have hindered the routine clinical use of this method. In a pediatric patient group, these factors are more perplexing. To circumvent these problems, we made use of nested amplification and developed a standard protocol for extracting DNA from various forms of clinical samples which were suitable to our clinical laboratory. It is our impression that the overall sensitivity, including technical bias accompanying this method, is equal to, or at least greater than, that of culture. Most notably, the rapidity in obtaining results and the simplicity in handling, storage and transfer of samples are the principal advantages of this method.

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Polymerase chain reaction for detection of Mycobacterium tuberculosis

Cited by 21 publications

References 16 publications

Children with significant cervical lymphadenopathy: clinicopathological analysis and role of fine-needle aspiration in Indian setup

Children with significant cervical lymphadenopathy: clinicopathological analysis and role of fine-needle aspiration in Indian setup

Tuberculous disseminated lymphadenopathy in an immunocompetent non-HIV patient: a case report

Nested amplification protocol for the detection of Mycobacterium tuberculosis

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