Polymerase chain reaction for detection of patent infections of Echinococcus granulosus (“sheep strain”) in naturally infected dogs

Štefanić, Saša; Shaĭkenov, B; Deplazes, Peter; Dinkel, Anke; Torgerson, Paul R.; Mathis, Alexander

doi:10.1007/s00436-003-1043-y

Cited by 155 publications

(126 citation statements)

References 19 publications

Supporting

Mentioning

123

Contrasting

Order By: Relevance

“…Dogs that were co-infected with E. multilocularis and E. granulosus were detected as reported in previous studies (Stefanic et al, 2004;Xiao et al, 2006). In these reports, co-infections with E. multilocularis and two different strains of E. granulosus were detected.…”

Section: Discussionsupporting

confidence: 72%

“…DNA isolation from purged worms and from taeniid eggs was as described previously (Stefanic et al, 2004). All faecal samples were analysed for the presence of taeniid eggs and positive samples were subsequently analysed by PCR.…”

Section: Dna Isolation Pcr and Cloningmentioning

confidence: 99%

“…All faecal samples were analysed for the presence of taeniid eggs and positive samples were subsequently analysed by PCR. Initially, a PCR-specific probe for E. granulosus G1 strain (Stefanic et al, 2004) was used on egg-DNA. During the study, a newly developed multiplex PCR for simultaneous detection of E. granulosus (all strains), E. multilocularis and Taenia spp.…”

Section: Dna Isolation Pcr and Cloningmentioning

confidence: 99%

“…Techniques based on taeniid egg isolation and subsequent DNA analysis using PCR have been developed for the diagnosis of echinococcosis in definitive hosts (Mathis et al, 1996;Stefanic et al, 2004;Trachsel et al, 2007). These will not detect prepatent infections and therefore will have a sensitivity of less than one.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Canine echinococcosis in Kyrgyzstan: Using prevalence data adjusted for measurement error to develop transmission dynamics models

Ziadinov

Mathis

Trachsel

et al. 2008

International Journal for Parasitology

View full text Add to dashboard Cite

Echinococcosis is a major emerging zoonosis in central Asia. A cross-sectional study of dogs in four villages in rural Kyrgyzstan was undertaken to investigate the epidemiology and transmission of Echinococcus spp. A total of 466 dogs were examined by arecoline purgation for the presence of Echinococcus granulosus and Echinococcus multilocularis. In addition, a faecal sample from each dog was examined for taeniid eggs. Any taeniid eggs found were investigated using PCR techniques (multiplex and single target PCR) to improve the diagnostic sensitivity by confirming the presence of Echinococcus spp. and to identify E. granulosus strains. A total of 83 (18%) dogs had either E. granulosus adults in purge material and/or E. granulosus eggs in their faeces as confirmed by PCR. Three genotypes of E. granulosus: G1, G4 and the G6/7 complex were shown to be present in these dogs through subsequent sequence analysis. Purge analysis combined with PCR identified 50 dogs that were infected with adult E. multilocularis and/or had E. multilocularis eggs in their faeces (11%). Bayesian techniques were employed to estimate the true prevalence, the diagnostic sensitivity and specificity of the procedures used and the transmission parameters. The sensitivity of arecoline purgation for the detection of echinococcosis in dogs was rather low, with a value of 38% (Credible intervals (CIs) 27-50%) for E. granulosus and 21% (CIs 11-34%) for E. multilocularis. The specificity of arecoline purgation was assumed to be 100%. The sensitivity of coproscopy followed by PCR of the isolated eggs was calculated as 78% (CIs 57-87%) for E. granulosus and 50% (CIs 29-72%) for E. multilocularis with specificity of 93% (CIs 88-96%) and 100% (CIs 97-100), respectively. The 93% specificity of the coprological-PCR for E. granulosus could suggest coprophagia rather than true infections. After adjusting for the sensitivity of the diagnostic procedures, the estimated true prevalence of infection of E. granulosus was 19% (CIs 15-25%) and the infection pressure in the dog population was estimated to be 0.29 infections per year (CIs 0.014-0.75). Logistic regression analysis failed to identify any significant risk factors for infections for E. granulosus. After adjusting for the sensitivity of the test procedures, the estimated true prevalence was 18% (CIs 12-30%). Dogs that were restrained had a significantly lower prevalence of E. multilocularis of 11% (CIs 6-29%) compared with 26% in free-roaming dogs (CIs 17-44%) and

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Dna Isolation Pcr and Cloningmentioning

confidence: 99%

Section: Dna Isolation Pcr and Cloningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Canine echinococcosis in Kyrgyzstan: Using prevalence data adjusted for measurement error to develop transmission dynamics models

Ziadinov

Mathis

Trachsel

et al. 2008

International Journal for Parasitology

View full text Add to dashboard Cite

show abstract

“…This was first detected in a study in 2002. Of 131 dogs 6 were shown to be infected with E. multilocularis (Stefanić et al 2004). One of these dogs was infected with both E. granulosus and E. multilocularis.…”

Section: Definitive Hostsmentioning

confidence: 99%

The emergence of echinococcosis in central Asia

Torgerson

2013

Parasitology

View full text Add to dashboard Cite

Following the collapse of the Soviet Union in 1991, there was an increase in the number of cases of human echinococcosis recorded throughout central Asia. Between 1991 and 2001 incidence rates of cystic echinococcosis (CE) increased by 4 fold or more. There also appeared to be increases in prevalence of CE in livestock and prevalences of Echinococcus granulosus reported in dogs. The increase in human echinococcosis was associated with changes in livestock husbandry, decline in veterinary public health services, increases in dog populations and increased poverty, all of which served to promote transmission of E. granulosus. A few years after reports of increased transmission of E. granulosus, the first reports of E. multilocularis infection in dogs were recorded. Further studies indicated that in both Kazakhstan and Kyrgyzstan prevalences of up to 18% were present. Recently there has been a dramatic increase in the number of cases of human alveolar echinococcosis recorded in Kyrgyzstan with over 60 cases reported in 2011.

show abstract

Cystic and Alveolar Echinococcosis: Fraternal Twins Both in Search of Optimal Treatment

Vuitton

Millon

Manciulli

et al. 2022

Zoonoses: Infections Affecting Humans and Animals

View full text Add to dashboard Cite

Polymerase chain reaction for detection of patent infections of Echinococcus granulosus (“sheep strain”) in naturally infected dogs

Cited by 155 publications

References 19 publications

Canine echinococcosis in Kyrgyzstan: Using prevalence data adjusted for measurement error to develop transmission dynamics models

Canine echinococcosis in Kyrgyzstan: Using prevalence data adjusted for measurement error to develop transmission dynamics models

The emergence of echinococcosis in central Asia

Cystic and Alveolar Echinococcosis: Fraternal Twins Both in Search of Optimal Treatment

Contact Info

Product

Resources

About