Polymerase chain reaction detection of Bartonella henselae bacteraemia in an immunocompetent child with cat-scratch disease

Fumarola, D; Ungari, S; Fumarola, Luciana; Miragliotta, G.

doi:10.1007/s004310051286

Cited by 15 publications

(8 citation statements)

References 28 publications

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“…Due to its fastidious nature, Bartonella henselae growth and isolation can be very difficult. Various culture media (e.g., rabbit blood and chocolate agars), the serological detection of Bartonella henselae antibodies by an indirect immunofluorescent assay, and other molecular techniques such as PCR are often utilized for its identification (54,55,56,190). Another Bartonella species recently implicated in the development of cat scratch disease due to a cat bite is Bartonella clarridgeiae (138).…”

Section: Less Commonly Observed (յ4%) Anaerobic Isolates Include Tannmentioning

confidence: 99%

Microbiology of Animal Bite Wound Infections

2011

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SUMMARY The microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. Bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. Bacteria may also originate from the victim's own skin or the physical environment at the time of injury. Our review has focused on bite wound infections in humans from dogs, cats, and a variety of other animals such as monkeys, bears, pigs, ferrets, horses, sheep, Tasmanian devils, snakes, Komodo dragons, monitor lizards, iguanas, alligators/crocodiles, rats, guinea pigs, hamsters, prairie dogs, swans, and sharks. The medical literature in this area has been made up mostly of small case series or case reports. Very few studies have been systematic and are often limited to dog or cat bite injuries. Limitations of studies include a lack of established or inconsistent criteria for an infected wound and a failure to utilize optimal techniques in pathogen isolation, especially for anaerobic organisms. There is also a lack of an understanding of the pathogenic significance of all cultured organisms. Gathering information and conducting research in a more systematic and methodical fashion through an organized research network, including zoos, veterinary practices, and rural clinics and hospitals, are needed to better define the microbiology of animal bite wound infections in humans.

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Section: Less Commonly Observed (յ4%) Anaerobic Isolates Include Tannmentioning

confidence: 99%

Microbiology of Animal Bite Wound Infections

2011

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“…It can be hypothesized that a low number of viable microorganisms persisting inside infected cells without replicating can account for relapses in susceptible animals. Persistent infection of liver and spleen by B. henselae may occasionally be observed in some immunocompetent CSD patients, mostly children, and may require up to 6 months for complete recovery (11,40). Cats, which are the natural host of B. henselae, may harbor the microorganism for at least 18 months, suggesting that some bacteria probably survive inside macrophage or endothelial cells.…”

Section: Henselae-j774 Interaction 5977mentioning

confidence: 99%

Interaction of Bartonella henselae with the Murine Macrophage Cell Line J774: Infection and Proinflammatory Response

Musso¹,

Badolato

Ravarino³

et al. 2001

Infect Immun

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“…Laboratory diagnosis is based on culture isolation, serology, histopathology, and molecular detection mainly in lymph node biopsies and aspirates, (which is probably due to a higher concentration of bacteria in these specimens), and more rarely in peripheral blood samples (HANSMANN et al, 2005;DEL PRETE et al, 2000;ARVAND & SCHÄD, 2006). Species identification and differentiation is troublesome due to the bacteria's slow and fastidious growth.…”

Section: Introductionmentioning

confidence: 99%

Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications

Kawasato

Oliveira

Velho

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARYBacteria of the genus Bartonella are emerging pathogens detected in lymph node biopsies and aspirates probably caused by increased concentration of bacteria. Twenty-three samples of 18 patients with clinical, laboratory and/or epidemiological data suggesting bartonellosis were subjected to three nested amplifications targeting a fragment of the 60-kDa heat shock protein (HSP), the internal transcribed spacer 16S-23S rRNA (ITS) and the cell division (FtsZ) of Bartonella henselae, in order to improve detection in clinical samples. In the first amplification 01, 04 and 05 samples, were positive by HSP (4.3%), FtsZ (17.4%) and ITS (21.7%), respectively. After the second round six positive samples were identified by nested-HSP (26%), eight by nested-ITS (34.8%) and 18 by nested-FtsZ (78.2%), corresponding to 10 peripheral blood samples, five lymph node biopsies, two skin biopsies and one lymph node aspirate. The nested-FtsZ was more sensitive than nested-HSP and nested-ITS (p < 0.0001), enabling the detection of Bartonella henselae DNA in 15 of 18 patients (83.3%). In this study, three nested-PCR that should be specific for Bartonella henselae amplification were developed, but only the nested-FtsZ did not amplify DNA from Bartonella quintana. We conclude that nested amplifications increased detection of B. henselae DNA, and that the nested-FtsZ was the most sensitive and the only specific to B. henselae in different biological samples. As all samples detected by nested-HSP and nested-ITS, were also by nested-FtsZ, we infer that in our series infections were caused by Bartonella henselae. The high number of positive blood samples draws attention to the use of this biological material in the investigation of bartonellosis, regardless of the immune status of patients. This fact is important in the case of critically ill patients and young children to avoid more invasive procedures such as lymph nodes biopsies and aspirates.

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Polymerase chain reaction detection of Bartonella henselae bacteraemia in an immunocompetent child with cat-scratch disease

Abstract: The polymerase chain reaction can be used for the rapid laboratory diagnosis of bacteraemia in cat-scratch disease.

Cited by 15 publications

References 28 publications

Microbiology of Animal Bite Wound Infections

Microbiology of Animal Bite Wound Infections

Interaction of Bartonella henselae with the Murine Macrophage Cell Line J774: Infection and Proinflammatory Response

Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications

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