Polymerase Chain Reaction–Denaturing Gradient Gel Electrophoresis, Cloning, and Sequence Analysis of Bacteria Associated with Acute Periapical Abscesses in Children

Yang, Qiu-Bo; Fan, Lu-Na; Shi, Qiong

doi:10.1016/j.joen.2009.11.001

Cited by 16 publications

(34 citation statements)

References 30 publications

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“…The presence of P. gingivalis has been shown in endodontic infections affecting approximately 27% of the deciduous teeth 8,27 ; however, in this study, this microorganism was observed in 49% of the analyzed necrotic pulps, and this result is similar to that found in adults 22 . In addition, pulp samples harbored P. nigrescens (11%), P. intermedia (6%) and P. endodontalis (11%), and, certainly, their association with other bacteria may contribute to the pathogenicity and maintenance of the infectious process.…”

Section: Discussionsupporting

confidence: 65%

“…Before collecting fistula sample, antisepsis with iodine (2%) for 1 min was performed. Sodium hypochlorite and iodine solutions were neutralized with sodium thiosulfate (5%) for 30 s. In dry root canal, one drop of sterile saline (0.9%) was added, avoiding flooding 27 . The paper points were left in wet canal for 60 s and then transferred to tubes containing 250 µL of TE.…”

Section: Methodsmentioning

confidence: 99%

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Bacteriological analysis of necrotic pulp and fistulae in primary teeth

2014

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Objectives Primary teeth work as guides for the eruption of permanent dentition, contribute for the development of the jaws, chewing process, preparing food for digestion, and nutrient assimilation. Treatment of pulp necrosis in primary teeth is complex due to anatomical and physiological characteristics and high number of bacterial species present in endodontic infections. The bacterial presence alone or in association in necrotic pulp and fistula samples from primary teeth of boys and girls was evaluated. Material and Methods Necrotic pulp (103) and fistula (7) samples from deciduous teeth with deep caries of 110 children were evaluated. Bacterial morphotypes and species from all clinical samples were determined. Results A predominance of gram-positive cocci (81.8%) and gram-negative coccobacilli (49.1%) was observed. In 88 out of 103 pulp samples, a high prevalence of Enterococcus spp. (50%), Porphyromonas gingivalis (49%), Fusobacterium nucleatum (25%) and Prevotella nigrescens (11.4%) was observed. Porphyromonas gingivalis was detected in three out of seven fistula samples, Enterococcus spp. in two out of seven samples, and F. nucleatum, P. nigrescens and D. pneumosintes in one out of seven samples. Conclusions Our results show that Enterococcus spp. and P. gingivalis were prevalent in necrotic pulp from deciduous teeth in boys from 2 to 5 years old, and that care of the oral cavity of children up to five years of age is important.

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Section: Discussionsupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

Bacteriological analysis of necrotic pulp and fistulae in primary teeth

2014

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“…17 The present study found the presence of P. micra, which formerly belonged to the genus Peptostreptococcus, in 40% of the RCs. P. micra is associated with the pathogenesis of P. gingivalis in endodontic abscesses.…”

Section: Discussionsupporting

confidence: 52%

An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The "red complex", which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.

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“…The Erysipelotrichales were also increased in the COPD analysis. This increase was also reflected at multiple taxonomic levels including the genus Bulleidia (Solobacterium), an organism implicated in periodontal disease and dental abscesses [23]. The Aeromonadales, an order of the gram-negative class Gammaproteobacteria, were increased in the COPD, ICS, IBD, and Severe (vs.…”

Section: Resultsmentioning

confidence: 98%

The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease

Pragman¹,

Kim²,

Reilly³

et al. 2014

Annals ATS

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Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. Bacterial infection of the lower respiratory tract contributes to approximately 50% of COPD exacerbations. Even during periods of stable lung function, the lung harbors a community of bacteria, termed the microbiome. The role of the lung microbiome in the pathogenesis of COPD remains unknown. The COPD lung microbiome, like the healthy lung microbiome, appears to reflect microaspiration of oral microflora. Here we describe the COPD lung microbiome of 22 patients with Moderate or Severe COPD compared to 10 healthy control patients. The composition of the lung microbiomes was determined using 454 pyrosequencing of 16S rDNA found in bronchoalveolar lavage fluid. Sequences were analyzed using mothur, Ribosomal Database Project, Fast UniFrac, and Metastats. Our results showed a significant increase in microbial diversity with the development of COPD. The main phyla in all samples were Actinobacteria, Firmicutes, and Proteobacteria. Principal coordinate analyses demonstrated separation of control and COPD samples, but samples did not cluster based on disease severity. However, samples did cluster based on the use of inhaled corticosteroids and inhaled bronchodilators. Metastats analyses demonstrated an increased abundance of several oral bacteria in COPD samples.

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Polymerase Chain Reaction–Denaturing Gradient Gel Electrophoresis, Cloning, and Sequence Analysis of Bacteria Associated with Acute Periapical Abscesses in Children

Cited by 16 publications

References 30 publications

Bacteriological analysis of necrotic pulp and fistulae in primary teeth

Bacteriological analysis of necrotic pulp and fistulae in primary teeth

An investigation of the presence of specific anaerobic species in necrotic primary teeth

The Lung Microbiome in Moderate and Severe Chronic Obstructive Pulmonary Disease

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