Polymerase chain reaction compared with concurrent viral cultures for rapid identification of human immunodeficiency virus infection among high-risk infants and children

Edwards, John; Ulrich, Paul P.; Weintrub, Peggy Sue; Cowan, Morfon J.; Levy, Jay A.; Wara, Diane W.; Vyas, Girish N.

doi:10.1016/s0022-3476(89)80065-4

Cited by 56 publications

(18 citation statements)

References 12 publications

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“…The transmission of HIV from mother to child appears to occur in 11 to 60% of children born to HIV-positive mothers (7,77,206,207,271,501,641,700,845,883,889,1010,1018,1049,1189). This prevalence is based primarily on PCR and virus culture studies; diagnosis by serology is difficult since maternal antibodies are present in infants at birth (for reviews, see references 883 and 1009).…”

Section: F Transmission From Mother To Childmentioning

confidence: 99%

Pathogenesis of Human Immunodeficiency Virus Infection

Levy¹

1989

Annals of the New York Academy of Sciences

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222

290

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Section: F Transmission From Mother To Childmentioning

confidence: 99%

Pathogenesis of Human Immunodeficiency Virus Infection

Levy¹

1989

Annals of the New York Academy of Sciences

Self Cite

222

290

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“…PBMC were evaluated for HIV by standard This noncytotoxic anti-HIV activity, when measured by the polymerase chain reaction (PCR) techniques [8].…”

mentioning

confidence: 99%

“…This anti-HIV response is demonstrated by suppression of viral replication at low CD8:CD4 cell ratios by use of 6 years later has indicated that 3 children (patients 7,8,32) are infected but remain asymptomatic. One other child has died non -HLA-matched target cells (table 1; figure 1).…”

mentioning

confidence: 99%

CD8 Cell Noncytotoxic Antiviral Activity in Human Immunodeficiency Virus‐Infected and ‐Uninfected Children

Levy¹,

Hsueh²,

Blackbourn³

et al. 1998

J INFECT DIS

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“…The use of polymerase chain reaction (PCR) has been of particular importance (1)(2)(3), being the method of choice for diagnosis of HIV-1 infection when anti-HIV-1 antibodies are present in low levels or may be passively acquired (4,5).…”

Section: Introductionmentioning

confidence: 99%

An optimized nested polymerase chain reaction (PCR) approach allows detection and characterization of human immunodeficiency virus type 1 (HIV‐1) env and gag genes from clinical samples

Locateli

Stoco

Zanetti

et al. 2008

Clinical Laboratory Analysis

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The needs for development and/or improvement of molecular approaches for microorganism detection and characterization such as polymerase chain reaction (PCR) are of high interest due their sensitivity and specificity when compared to traditional microbiological techniques. Considering the worldwide importance of human immunodeficiency virus type 1 (HIV-1) infection, it is essential that such approaches consider the genetic variability of the virus, the heterogeneous nature of the clinical samples, the existence of contaminants and inhibitors, and the consequent needs for standardization in order to guarantee the reproducibility of the methods. In this work we describe a nested PCR assay targeting HIV-1 virus gag and env genes, allowing specific and sensitive diagnosis and further direct characterization of clinical samples. The method described herein was tested on clinical samples and allowed the detection of HIV-1 presence in all samples tested for the gag gene and 90.9% for the env gene, revealing sensitivities of 1 fg and 100 fg, respectively. Also, no cross-reactions were observed with DNA from infected and noninfected patients and the method allowed detection of the env and gag genes on an excess of 10(8) and 10(4) of human deoxyribonucleic acid (DNA), respectively. Furthermore, it was possible to direct sequence all amplified products, which allowed the sub typing of the virus in clinical samples.

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Polymerase chain reaction compared with concurrent viral cultures for rapid identification of human immunodeficiency virus infection among high-risk infants and children

Cited by 56 publications

References 12 publications

Pathogenesis of Human Immunodeficiency Virus Infection

Pathogenesis of Human Immunodeficiency Virus Infection

CD8 Cell Noncytotoxic Antiviral Activity in Human Immunodeficiency Virus‐Infected and ‐Uninfected Children

An optimized nested polymerase chain reaction (PCR) approach allows detection and characterization of human immunodeficiency virus type 1 (HIV‐1) env and gag genes from clinical samples

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