Polymerase Chain Reaction-Based Identity Assay for Pathogenic Turkey Eimeria

Cook, Stephanie; Higuchi, Deborrah S.; McGowan, Alexandria L.; Schrader, Joan S.; Withanage, G. S. K.; Francis, Michael J.

doi:10.1637/9531-927110-digest.1

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“…An ITS-1-based PCR has also been developed for the diagnosis of turkey Eimeria (Cook et al 2010). Divergent paralogs of nuclear rDNA of Eimeria have been reported in chicken (Vrba et al 2011) andturkeys (El-Sherry et al 2013), and due to the polymorphism seen in ITS rDNA (Lew et al 2003), a PCR based on amplification of ITS could result in reduced sensitivity or could provide false negative results (Morgan et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…For example, an overlap in oocyst dimensions has been shown for Eimeria adenoeides, Eimeria meleagrimitis, and Eimeria meleagridis (Clarkson, 1960). Molecular-based identification methods are gaining in popularity, and several assays have been developed for chicken (Schnitzler et al 1998(Schnitzler et al , 1999Lew et al 2003) and more recently turkey Eimeria (Cook et al 2010). The objective of this study was to ascertain the prevalence of Eimeria species obtained from 11 major turkey growing states throughout the USA using the species-specific polymerase chain reaction (PCR) amplification of mitochondrial cytochrome c oxidase subunit I (COI) gene.…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of field isolates of Turkey Eimeria by polymerase chain reaction amplification of the cytochrome c oxidase I gene

2015

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Oocysts of Eimeria spp. were isolated from litter samples obtained from 30 commercial turkey farms. Genomic DNA was extracted from clean oocysts, and polymerase chain amplification of the species-specific cytochrome c oxidase subunit I (COI) gene was performed for five species of turkey Eimeria. The species tested were Eimeria adenoeides, Eimeria meleagrimitis, Eimeria meleagridis, Eimeria dispersa, and Eimeria gallopavonis. All DNA samples were positive for E. meleagrimitis, nine were positive for E. adenoeides, two were positive for E. dispersa, and none for E. meleagridis and E. gallopavonis. E. meleagrimitis occurred as a single species in 21 (70 %) of the farms while 9 (30 %) farms had a mixed species with E. meleagrimitis and E. adenoeides and 2 (7 %) were triple positive with E. meleagrimitis, E. adenoeides, and E. dispersa. This is the first account of the field prevalence of turkey Eimeria species using molecular methods.

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