Polymerase chain reaction based detection of banana bunchy top virus using coat protein based primers

Recombinase polymerase amplification (RPA) is a rapid, isothermal amplification method with high specificity and sensitivity. In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both in RPA as well as in polymerase chain reaction (PCR). A total of 133 symptomatic as well as asymptomatic banana leaf samples from various cultivars were collected from the different regions of India and evaluated for BBTV infection using the RPA assay. BBTV was efficiently detected using crude leaf sap in RPA and the results obtained were consistent with PCR-based detection using purified DNA as template. To our knowledge, this is the first report of reliable diagnosis of BBTV infection by RPA using crude leaf sap as a template.

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Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars

Kapoor

Srivastava

Kumar

et al. 2017

Arch Virol

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Polymerase chain reaction based detection of banana bunchy top virus using coat protein based primers

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References 12 publications

Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars

Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars

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