Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation

Rahman, Mohd Muhiden Abd; Ali, Eaqub; Hamid, Sharifah Bee Abd; Mustafa, Shuhaimi; Hashim, U.; Hanapi, Ummi Kalthum

doi:10.1016/j.meatsci.2014.03.011

Cited by 80 publications

(73 citation statements)

References 27 publications

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“…Doosti et al (2014) investigated the PCR-RFLP analysis of the mitochondrial cyt b gene to differentiate between beef, sheep, pork, chicken, donkey, and horse meats in meat products (sausages, frankfurters, hamburgers, hams and cold cut meats) and suggested that this method provide a potential technique to rely on for authentication of halal (lawful or permitted) meat origin. Rahman et al (2014) assessed the presence of dog meat in meatball by PCR assay for amplification of 100-bp region of canine mitochondrial cyt b gene in different circumstances (pure, raw, processed and mixed conditions). This assay tested with many other animal and plant species used in the formation of meatball and is found to be simple, stable, sensitive and specific to detect dog meat in processed food which is very important for halal authentication purposes.…”

Section: Restriction Fragment Length Polymorphism (Pcr-rflp)mentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

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| Identification of animal species of origin in meat and meat products is a matter of great concerns such as religious, economical, legal as well as medical aspects. Thus, several analytical techniques have been suggested for the identification of meat species either in individual or in mixed samples to protect consumers from the fraudulent and bad habits of marketing. DNA-based techniques especially the techniques based on polymerase chain reaction (PCR) are recognized as the most appropriate methods employed for species identification in raw and processed meat. PCR techniques including randomly amplified polymorphic DNA (PCR-RAPD), restriction fragment length polymorphism (PCR-RFLP), PCR with species-specific primers, real-time PCR and PCR-nucleotide sequencing allow identification of meat species under different processing conditions. But the variability of DNA content on the level of species as well as target tissue make the DNA-based methods somewhat unsuitable for the quantification of exact percentages of different species in meat and meat products. For these reasons the proteomic approaches depending on identification of different peptide biomarkers has been developed and employed to give information on the different composition of food. To broad the knowledge about these technologies, this review is compiled in an attempt to provide an overview of the possible PCR-based analytical techniques that could help in identifying the meat species of origin in meat and meat products and threw the light on the identification of species specific peptide biomarkers by proteomic technologies as a new and attractive alternative that could overcome some of the limitations that faced DNAbased methods especially when used for meat exposed to intensive heating of processing as well as for meat mixtures.

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Section: Restriction Fragment Length Polymorphism (Pcr-rflp)mentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

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“…One of the potential meat used for beef adulteration is dog meat. Dog meat (DM) is a potential adulterant as in certain countries like Indonesia DM distribution and price are not well-regulated and thus can be bought at a cheaper price [5]. In addition, some communities in China, Myanmar, South Korea and Vietnam consume dog meats [6].…”

Section: Introductionmentioning

confidence: 99%

“…Rahman et al [5] have used PCR assay using cytochrome b gene as a target for detecting DM adulteration in meatball formulation. PCR assay using specific-specific primer on cytochrome b was also used for authentication of commercial Frankfurters from DM [12].…”

Section: Introductionmentioning

confidence: 99%

Application of FTIR Spectroscopy and Chemometrics for Halal Authentication of Beef Meatball Adulterated with Dog Meat

et al. 2018

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“…Thus, meat species authentication has become an important issue and timely need to secure consumers' trust on labeled brands (Ali et al 2012c;Rohman et al 2011). Recently, DNA-based techniques such as PCR (Rahman et al 2014), PCR-RFLP (Ali et al 2012b), and DNA barcoding (Wong and Hanner 2008) have become the method of choice because of the higher stability and universal availability of the DNA molecule itself (Pereira et al 2008). PCR-RFLP is particularly interesting in this endeavour since it allows the confirmation of the authentic PCR products through the analysis of the restriction-digested PCR products (Ali et al 2012b).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, availability of stray dogs in certain countries (Kumarapeli and Awerbuch-Friedlander 2009;Totton et al 2010) has made dog meat a potential adulterant in costly meats such as beef, lamb, and chicken. Henceforth, several PCR assays for canine species detection have been documented (Abdel-Rahman et al 2009;Abdulmawjood et al 2003;Ali et al 2013;Arslan et al 2006;Gao et al 2004;İlhak and Arslan 2007;Martín et al 2007;Rahman et al 2014). However, all of these assays are based on traditional agarose-gel electrophoresis for PCR endpoint detection, and none of them have been tested in burger formulation.…”

Section: Introductionmentioning

confidence: 99%

Lab-on-a-Chip PCR-RFLP Assay for the Detection of Canine DNA in Burger Formulations

et al. 2015

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Canine species detection in foods is important in the perspectives of health, religions, and fare-trade food business. This study describes a very short-amplicon length Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) assay with lab-on-a-chip detection platform for the authentication of canine DNA in processed foods. A 100-bp fragment of canine mitochondrial Cytochrome b (cytb) gene was selected and amplified using a pair of canine-specific primers. The amplified PCR products were validated by RFLP analysis using lab-on-a-chip microfluidic bioanalyzer kit. Both gel-image and electropherograms authenticated the canine-specific PCR products before (100 bp) and after restriction digestion (51, 30, and 19 bp). The assay successfully detected 0.0001-ng canine DNA under pure state and 0.01 % (w/w) canine meat spiked in chicken and beef burger formulations. Screening of eight commercial burgers across Malaysia did not reveal any canine adulteration. We believe the assay would find potential applications in food industries, Halal food regulatory bodies and animal right protection authorities across the globe.

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Polymerase chain reaction assay targeting cytochrome b gene for the detection of dog meat adulteration in meatball formulation

Cited by 80 publications

References 27 publications

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Application of FTIR Spectroscopy and Chemometrics for Halal Authentication of Beef Meatball Adulterated with Dog Meat

Lab-on-a-Chip PCR-RFLP Assay for the Detection of Canine DNA in Burger Formulations

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