Polymerase chain reaction assay in acute urethritis

Chlamydia trachomatis is a microorganism known for years to cause ocular, urogenital, and neonatal infections in humans. It usually causes urogenital system infections. The pathogen, which is the most common cause of urethritis in males, is one of the sexually transmitted microorganisms. As most males are asymptomatic, they do not realize they are infected and act as reservoirs. This causes the incidence of urethritis due to chlamydia to increase day by day. Chlamydia urethritis, which poses a risk to sexual partners, can cause serious complications if left untreated. In this section, we assess the approach to male urethritis due to chlamydia, which is very common in urology practice and can cause serious problems if left untreated.

Section: Discussionmentioning

confidence: 99%

Section: Epidemiologymentioning

confidence: 99%

Chlamydia Infection from Androgical Perspective

Duman¹

2023

“…Compared with AF genital tract multiplex PCR, its sensitivity is low in male patients with previous acute urethritis [33]. Urethritis in men is one of the most common sexually transmitted diseases, and although there have been important developments in treatment and diagnosis in recent years, the most common method is the use of polymerase chain reactions [34].…”

Section: Use Of Molecular Techniques In Chlamydia Diagnosis and Scree...mentioning

confidence: 99%

Molecular Approaches to the Diagnosis of Chlamydia

Konuk¹

2023

Chlamydia trachomatis is known as the most common bacterial infection agent to pass with sexual transition. This microorganism is an obligatory intracellular parasite. A variety of infections are caused by C. trachomatis, including trachoma, pneumonias in newborns, genital and urinary tract infections, and lymphogranuloma venereum (LGV), which is caused by LGV strains. The diagnosis of Chlamydia trachomatis can be made by cultures and isolations, antigens and antibodies (direct fluorescence, enzyme immunoassays), hybridization, or polymerase chain reaction (PCR). Each year, infection and diagnosis rates increase in the developed world. Since Chlamydia is mostly asymptomatic, screening, and treatment are a key to detecting cases. Polymerase chain reaction (PCR), ligase chain reaction (LCR), and nucleic acid sequence-based amplification (NASBAa) molecular methods can be used for the detection, low concentration, quantification, and identification of organisms. While the traditional PCR method confirms its existence, it can quantify real-time PCR (RT-PCR). This method (RT-PCR) may have low sensitivity among variants of the same species. Also, PCR scans, which receive urine service, offer great advantages. PCR from initial void urine (FVU) samples is highly sensitive in detecting the organism. Urine Chlamydia screenings are more acceptable in large populations and asymptomatic detections.

“…Methods used in diagnosis include cytological examination, isolation in cell culture, antigen determination, serological examinations, and molecular methods in which nucleic acid of the agent is detected [8,57,58]. NAATs are one of the most sensitive tests used in the examination of samples [59,60] and are recommended for use in diagnosing chlamydia infection [38]. C. trachomatis culture has been generally abandoned.…”

Section: Rectal C Trachomatis Infectionmentioning

confidence: 99%

Chlamydia and the Gastrointestinal System

Alkan¹

2023

Chlamydiae are intracellular, gram-negative, and prokaryotic microorganisms. Capable of causing disease in many mammalian and avian species, there are three types that cause disease in humans: Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci. Among the chlamydia species, C. trachomatis is the most studied and encountered type because it is a leading cause of trachoma and sexually transmitted diseases. C. trachomatis, a known pathogen of the genital tract, can also be routinely detected in the human gastrointestinal tract. It can infect the enteroendocrine cells of the gastrointestinal tract. The best-known manner for C. trachomatis to enter the gastrointestinal tract is through oral and anal sex. Most of them are dormant, without causing any infection in the infected person. Chlamydia proctitis is the most well-known disease caused by C. trachomatis in the gastrointestinal tract. In this section, we evaluated the often-overlooked Chlamydia and the gastrointestinal system findings within the gastroenterology practice, the diseases it causes, and the treatments for these diseases.