Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii

Ellis, John

doi:10.1016/s0020-7519(98)00096-4

Cited by 71 publications

(37 citation statements)

References 51 publications

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“…A mononuclear inflammatory infiltrate was predominant in the heart, kidney, liver, lung, brain and placenta in 75 fetuses, suggesting abortion due to protozoa, but none was positive to T. gondii by PCR. These findings are in agreement with a study in Argentina (moore et al, 2008) where none of the 70 fetuses were positive by nested-PCR, although low rates of T. gondii infection have been reported in other countries (ellis, 1998;GoTTsTein et al, 1998;saGer et al, 2001;CanaDa et al, 2002;reiTT et al, 2007). Cabral et al (2009), using the same 105 aborted fetuses of this study, confirmed the presence of N. caninum in 24.8% by IHC and nested PCR.…”

Section: Palavras-chavesupporting

confidence: 93%

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

et al. 2013

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The aim of this study was to determine if Toxoplasma gondii was present in aborted bovine fetuses in Brazil. Histopathology of 105 cases with suspected infectious abortion, analyzed during the period from 2006 to 2008 at Centro de Pesquisa e Desenvolvimento de Sanidade Animal of Instituto Biológico, São Paulo, showed 75 cases with indications of abortion due to apicomplexan protozoa. These cases were submitted to PCR for verification of the laboratory diagnosis. Fetal DNA was extracted from central nervous system, heart, liver, muscle, and/or placenta samples to obtain a 529 bp DNA fragment. T. gondii DNA was not detected in any of the bovine fetuses analyzed, suggesting that it may not be a frequent cause of bovine abortion.

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Section: Palavras-chavesupporting

confidence: 93%

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

et al. 2013

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“…The related parasite T. gondii is responsible for abortions and congenital infections in sheep, goats (for review see [7]) and humans [40] and disseminated toxoplasmosis is a severe disease in immunocompromised patients. However, T. gondii is not regarded as abortifacient in cattle although T. gondii specific DNA was recently detected by polymerase chain reaction (PCR) in damaged foetal brains, showing histo-pathological lesions consistent with a protozoan infection [18,20].…”

Section: Introductionmentioning

confidence: 99%

“…IFAT [5] or antigen-capture and inhibition ELISAs [4,6,23] are widely used. PCR and DNA hybridization assays are mainly used in research laboratories to demonstrate the presence of N. caninum DNA in tissues [18,42]. However, the suitability of these methods for practical use on autolysed or mummified foetuses needs to be further evaluated [45].…”

Section: Introductionmentioning

confidence: 99%

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

et al. 2002

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-Neospora caninum and Toxoplasma gondii are cyst-forming coccidian parasites of human and veterinary clinical relevance. In vitro cultivation of the protozoans using Vero cells is usually performed in order to produce antigenic materials. Quantitative and qualitative comparisons of Vero cells grown in RPMI medium supplemented either with foetal calf serum (FCS), horse serum (HS) or a specific serum-free additive (DefCell) were performed. A serum-free cell culture system used to propagate N. caninum (NC-1 isolate) and T. gondii tachyzoites (Rh stain) were compared with the other two cell culture systems. FCS supplemented media was found to be more effective than the others in promoting Vero cells and N. caninum tachyzoites. However, it was found unable to support adequate T. gondii tachyzoite proliferation. Vero cells, T. gondii and N. caninum tachyzoite production gave similar growth patterns with either HS or DefCell supplemented media. Defcell was considered as a good alternative to supplement culture medium.Neospora caninum / Toxoplasma gondii / cell culture / serum-free medium Résumé -Utilisation d'un milieu sans sérum pour la production in vitro de tachyzoites de Neospora caninum et Toxoplasma gondii sur cellules Vero. Neospora caninum et Toxoplasma gondii sont deux sporozoaires présentant un intérêt en médecine humaine et vétérinaire. La culture in vitro utilisant, entre autres, les cellules Vero comme support de la multiplication des deux parasites, est généralement employée en vue de l'obtention de tachyzoïtes. Au cours de cette étude, une évaluation quantitative et 159 Vet. Res. 33 (2002) 159-168

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“…Because Pitel et al (2001) used naturally aborted fetuses, their infection should be more severe than that in the fetuses used in the present study, and neosporosis was diagnosed by PCR because the parasite count was higher. In fact, PCR was not sensitive enough to detect neosporosis in other studies (ELLIS, 1998) probably because very few protozoa were present in the tissues of the infected animals (DUBEY et al, 1988;THURMOND;HIETALA, 1999).…”

Section: Discussionmentioning

confidence: 94%

“…It is possible that the method proposed by Ellis (1998) and Holmdahl, Mattson (1996), who found a high specificity and sensitivity of nested PCR for N. caninum diagnosis, should have been applied in this study. According to these authors, the internal transcription spacer of ribosomal DNA is ideal for PCR use because it has a high copy number and keeps the sequence of bases of the species well described for N. caninum.…”

Section: Discussionmentioning

confidence: 99%