Polymerase Chain Reaction and Immunoassay−Matrix Assisted Laser Desorption Mass Spectrometry Using Tag-Mass Technology: New Tools to Break Down Quantification Limits and Multiplexes

Stauber, Jonathan; Ayed, Mohamed El; Wisztorski, Maxence; R, Day; Fournier, Isabelle

doi:10.1021/ac901416s

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…Antibody mass tags have been effectively used for the development of MALDI/LDI-MS ELISA and microarray platforms. These early examples demonstrate the two most prominent mass tag design strategies: ortho -nitrobenzyl derivatives that can be incorporated into solid-phase peptide syntheses; and triphenylmethyl (trityl) tags, which fragment into cationic reporter ions, which can be detected in the absence of a MALDI matrix [ 73 , 77 , 78 ]. Comparatively more work has focussed on the application of these concepts for specific MSI, which is discussed in detail in Section 2.4.2 .…”

Section: Combined Immunochemical and Mass Spectrometric Approaches Tomentioning

confidence: 99%

Conjugating immunoassays to mass spectrometry: Solutions to contemporary challenges in clinical diagnostics

Stevens

Pukala

2020

TrAC Trends in Analytical Chemistry

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Developments in immunoassays and mass spectrometry have independently influenced diagnostic technology. However, both techniques possess unique strengths and limitations, which define their ability to meet evolving requirements for faster, more affordable and more accurate clinical tests. In response, hybrid techniques, which combine the accessibility and ease-of-use of immunoassays with the sensitivity, high throughput and multiplexing capabilities of mass spectrometry are continually being explored. Developments in antibody conjugation methodology have expanded the role of these biomolecules to applications outside of conventional colorimetric assays and histology. Furthermore, the range of different mass spectrometry ionisation and analysis technologies has enabled its successful adaptation as a detection method for numerous clinically relevant immunological assays. Several recent examples of combined mass spectrometry-immunoassay techniques demonstrate the potential of these methods as improved diagnostic tests for several important human diseases. The present challenges are to continue technological advancements in mass spectrometry instrumentation and develop improved bioconjugation methods, which can overcome their existing limitations and demonstrate the clinical significance of these hybrid approaches.

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Section: Combined Immunochemical and Mass Spectrometric Approaches Tomentioning

confidence: 99%

Conjugating immunoassays to mass spectrometry: Solutions to contemporary challenges in clinical diagnostics

Stevens

Pukala

2020

TrAC Trends in Analytical Chemistry

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“…In the TagMass method, a specifically designed antibody against a target antigen64 65 is linked to a laser-cleavable peptide64–68 with known sequence 64 65. An MS image of the tissue is generated.…”

Section: Other Techniquesmentioning

confidence: 99%

Next-generation protein analysis in the pathology department

et al. 2019

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Traditionally, immunohistochemistry (IHC) is used by pathologists to localise specific proteins or peptides in tissue slides. In the era of personalised medicine, however, molecular tissue analysis becomes indispensable for correct diagnosis, prognosis and therapeutic decision, not only on the DNA or mRNA level but also on the protein level. Combining molecular information with imaging presents many advantages. Therefore, matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI IMS) is a promising technique to be added to the armamentarium of the pathologist. Here, we focus on the workflow, advantages and drawbacks of both MALDI IMS and IHC. We also briefly discuss a few other protein imaging modalities and give examples of applications.

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“…The indirect detection methods take advantage of the MS ability to detect and differentiate among fairly small molecular tags that can be cleaved from NAs to identify a specific target that may itself not be amenable to MS because of size constraints and is illustrated in Figure 3B. A recent development is based on using a photocleavable linker with tagged molecules for PCR and ELISA quantification coupled to MS [43]. However, the effective application of the mass-tag methods to the multiplex detection of pathogens is still awaiting further validation.…”

Section: Pathogen Detection and Identificationmentioning

confidence: 99%

Application of mass spectrometry to molecular diagnostics of viral infections

Ganova-Raeva¹,

Khudyakov

2013

Expert Review of Molecular Diagnostics

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Mass spectrometry (MS) has found numerous applications in life sciences. It has high accuracy, sensitivity and wide dynamic range in addition to medium-to high-throughput capabilities. These features make MS a superior platform for analysis of various biomolecules including proteins, lipids, nucleic acids and carbohydrates. Until recently, MS was applied for protein detection and characterization. During the last decade, however, MS has successfully been used for molecular diagnostics of microbial and viral infections with the most notable applications being identification of pathogens, genomic sequencing, mutation detection, DNA methylation analysis, tracking of transmissions, and characterization of genetic heterogeneity. These new developments vastly expand the MS application from experimental research to public health and clinical fields. Matching of molecular techniques with specific requirements of the major MS platforms has produced powerful technologies for molecular diagnostics, which will further benefit from coupling with computational tools for extracting clinical information from MS-derived data.

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Polymerase Chain Reaction and Immunoassay−Matrix Assisted Laser Desorption Mass Spectrometry Using Tag-Mass Technology: New Tools to Break Down Quantification Limits and Multiplexes

Cited by 11 publications

References 32 publications

Conjugating immunoassays to mass spectrometry: Solutions to contemporary challenges in clinical diagnostics

Conjugating immunoassays to mass spectrometry: Solutions to contemporary challenges in clinical diagnostics

Next-generation protein analysis in the pathology department

Application of mass spectrometry to molecular diagnostics of viral infections

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