Polymer vesicles in vivo: correlations with PEG molecular weight

Photos, Peter J.; Bačáková, Lucie; Discher, Bohdana M.; Bates, Frank S.; Discher, Dennis E.

doi:10.1016/s0168-3659(03)00201-3

Cited by 495 publications

(499 citation statements)

References 52 publications

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“…An increase in the fraction of PEG2kBiotin present resulted in increased binding ( Figure 3B), however PEG2kBiotin at a concentration of 15% reduced the microbubble concentration by 30% as compared to a total PEG lipid concentration of 10%. PEG lipids at a high concentration have the potential to destabilize the microbubble lipid shell [39,40]. As a result, the concentration of PEG2k and PEG2kBiotin was 5% in all experiments.…”

Section: Peg2k/peg2kbiotin Ratio In Biotinylated Microbubble-the Molamentioning

confidence: 99%

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Kheirolomoom

Dayton

Lum

et al. 2007

Journal of Controlled Release

206

174

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A new acoustically-active delivery vehicle was developed by conjugating liposomes and microbubbles, using the high affinity interaction between avidin and biotin. Binding between microbubbles and liposomes each containing 5% DSPE-PEG2kBiotin was highly dependent on avidin concentration and observed above an avidin concentration of 10 nM. With an optimized avidin and liposome concentration, we measured and calculated as high as 1000 to 10,000 liposomes with average diameters of 200 and 100 nm, respectively, attached to each microbubble. Replacing avidin with neutravidin resulted in 3-fold higher binding, approaching the calculated saturation level. Highspeed photography of this new drug delivery vehicle demonstrated that the liposome-bearing microbubbles oscillate in response to an acoustic pulse similar to microbubble contrast agents. Additionally, microbubbles carrying liposomes could be spatially concentrated on a monolayer of PC-3 cells at the focal point of ultrasound beam. As a result of cell-vehicle contact, the liposomes fused with the cells and internalization of NBD-cholesterol occurred shortly after incubation at 37°C, with internalization of NBD-cholesterol substantially enhanced in the acoustic focus.

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Section: Peg2k/peg2kbiotin Ratio In Biotinylated Microbubble-the Molamentioning

confidence: 99%

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Kheirolomoom

Dayton

Lum

et al. 2007

Journal of Controlled Release

206

174

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“…Diblock copolymers of PEO 30 -b-PBD 46 and PEO 40 -b-PEE 37 were utilized in order to generate polymersomes of similar membrane corethickness (ℓ= 9.6 vs. 8 nm, respectively). 30 By comparing relative normalized emission intensities for PZn 3 A (green) and B (red) within these two related vesicle systems (Figure 5), the importance of inter-chain packing effects and fluorophore-fluorophore π-π interactions in determining vesicular PZn 3 emissive output can be assessed.…”

Section: Fluorophore Loading Within Saturated and Fully Unsaturated Bmentioning

confidence: 99%

Controlling Bulk Optical Properties of Emissive Polymersomes through Intramembranous Polymer−Fluorophore Interactions

Ghoroghchian

Frail

et al. 2007

Chem. Mater.

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Interdisciplinary investigation at the interface of chemistry, engineering, and medicine has enabled the development of self-assembled nanomaterials with novel biochemical and electro-optical properties. We have recently shown that emissive polymersomes, polymer vesicles incorporating porphyrin-based fluorophores, feature large integrated-emission oscillator strengths and narrow emission bands; these nanoscale assemblies can be further engineered to fluoresce at discrete wavelengths throughout the visible and near-infrared (NIR) spectral domains. As such, emissive polymersomes effectively define an organic-based family of soft-matter quantum-dot analogs that possess not only impressive optical properties, but also tunable physical and biomaterial characteristics relative to inorganic fluorescent nanoparticles.Here, we expand upon our initial studies on poly(ethyleneoxide)-block-poly(butadiene)-based vesicles to examine fluorophore membrane-loading in other polymersome systems. Through modulation of fluorophore ancilliary group substituents and choice of polymer chain chemistries, we are able to predictably control intramembranous polymer-fluorophore interactions; these phenomena, in turn, influence the nature of fluorophore solvation, local dielectric environment, and emission quantum yield within emissive polymersome assemblies. By utilizing different classes of vesiclegenerating diblock copolymers, including bioresorbable poly(ethyleneoxide)-block-poly(ε-caprolactone) (PEO-b-PCL) and poly(ethyleneoxide)-block-poly(γ-methyl-ε-caprolactone) (PEO-b-PMCL), we ascertain general principles important for engineering nanoscale optical vesicles. Further, this work heralds the first generation of fully-biodegradable fluorescent nanoparticles suitable for deep-tissue in vivo imaging.

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“…Polymersomes, polymer vesicles self-assembled from a diverse array of synthetic amphiphilic block copolymers containing hydrophilic and hydrophobic blocks [2][3][4], have been shown to possess superior biomaterial properties, including greater stability and storage capabilities [5][6][7], as well as prolonged circulation time, as compared to liposomes (vesicles derived from phospholipids) [8]. A particularly attractive storage feature, highlighted in Fig.…”

Section: Introductionmentioning

confidence: 99%

Polymersomes: A new multi-functional tool for cancer diagnosis and therapy

Levine

Ghoroghchian

Freudenberg

et al. 2008

Methods

195

121

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Nanoparticles are being developed as delivery vehicles for therapeutic pharmaceuticals and contrast imaging agents. Polymersomes (mesoscopic polymer vesicles) possess a number of attractive biomaterial properties that make them ideal for these applications. Synthetic control over block copolymer chemistry enables tunable design of polymersome material properties. The polymersome architecture, with its large hydrophilic reservoir and its thick hydrophobic lamellar membrane, provides significant storage capacity for both water soluble and insoluble substances (such as drugs and imaging probes). Further, the brush-like architecture of the polymersome outer shell can potentially increase biocompatibility and blood circulation times. A further recent advance is the development of multi-functional polymersomes that carry pharmaceuticals and imaging agents simultaneously. The ability to conjugate biologically active ligands to the brush surface provides a further means for targeted therapy and imaging. Hence, polymersomes hold enormous potential as nanostructured biomaterials for future in vivo drug delivery and diagnostic imaging applications.

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Polymer vesicles in vivo: correlations with PEG molecular weight

Cited by 495 publications

References 52 publications

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Controlling Bulk Optical Properties of Emissive Polymersomes through Intramembranous Polymer−Fluorophore Interactions

Polymersomes: A new multi-functional tool for cancer diagnosis and therapy

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