Polymer-Tethered Bimolecular Lipid Membranes

Synthesis of robust and high-performance aquaporin-based biomimetic membranes by interfacial polymerization-membrane preparation and RO performance characterization

“…Several designs have been proposed in the recent literature for producing ABMs, including polymer tethered bio-layers [11], biomembrane aperture partition arrays [12][13][14][15][16][17][18][19], membrane supported lipid bilayer via vesicle fusion [20][21], and vesicles suspended over membrane pores [22]. In addition, bio-nano fused membranes with polymerized proteoliposomes have also been prepared [23].…”

Recent Advances in Hybrid Biomimetic Polymer-Based Films: from Assembly to Applications

“…Polymer-tethered lipid bilayer membranes are based on supramolecular assembly of architectural elements, including solid support, polymeric tethers, and fluid lipid bilayer decorated if needed by versatile biomolecules [296]. Once the polymeric tethers are covalently attached to a solid support, the fluid lipid bilayer architecture is completed to have polymer-tethered lipid bilayer membrane either by transfer of the lipid monolayers via LB or LS techniques or vesicle fusion (Figure 8B in Section 3.2).…”

“…When the β-amyloid cleaving enzyme (BACE), which plays an active role in Alzheimer's Disease, is incorporated to those polymer-cushioned lipid bilayers with varying physicochemical properties, the cushioning of supported lipid membrane leads to an increase in the incorporation and enzymatic activity of the reconstituted BACE with a direct correlation between lipid mobility and BACE activity [330]. Moreover, polyelectrolyte cushions composed of multilayers of PAH and PSS have been adsorbed on functionalized substrates, followed by the fusion of lipid vesicles made of dimyristoyl-l-α-phosphatidylglycerol (DMPG) and DMPC to complete polyelectrolyte-cushioned membranes [296]. PEG is the most commonly used polymer cushion and can create a reservoir as thick as 10 nm under the membranes [323], and thus such a platform is preferable for the incorporation of transmembrane proteins.…”

Bilayer membrane interactions with nanofabricated scaffolds

“…The surface chemistry of molecular scaffolds can be precisely engineered to control the coupling of the bilayer to the surface, the spacer molecular length and shape, and the chemical functionality along the backbone, such as degree of hydrophobicity or hydrophilicity, the inclusion of saturated versus unsaturated bonds, and the presence or absence of bulky side groups. 33 Examples include linkers that can bind to silanol groups on silica to form siloxane linkages, 34 the formation of amide bonds at the substrate surface due to reaction of amine-coated glass with carboxylate groups or N-hydroxy succinimide (NHS) esters, 35 coupling of tethered biotin with streptavidin, 36 or spacers with pendant nickel nitrilotriacetic acid (Ni-NTA) groups for bonding molecules with polyhistidine tags. 30 Linker molecules could be based on DNA strands, 37 which offer greater specificity in binding biomolecules tagged with complementary sequences.…”