“…The transfection efficiency of the non-viral systems is not comparable to the efficiency of the viral delivers (Kodama, Katayama, Shoji, & Nakashima, 2006;Strand et al, 2005). Therefore, the relatively high transfection efficiency of the gas-filled microbubbles is noteworthy (Cavalier et al, 2008;Cheng, Xia, & Chan, 2004;Lentacker et al, 2006;Marmottant & Hilgenfeldt, 2003;Mehier-Humbert, Bettinger, Yan, & Gyu (2005); Mehier-Humbert et al, 2007;Suzuki et al, 2007;Taniyama et al, 2002). The gas-filled microbubbles are ultrasound contrast particles for molecular imaging (Klibanov, 2005), which can be utilized in gene delivery through the ultrasound-induced cavitation technique.…”